CHROMATIN DYNAMICS REGULATE GENOME FUNCTION
Grant number: 1124762 | Funding period: 2017 - 2021
Nuclear architecture is critical to the preservation of genome integrity. The aim of this research proposal is to delineate the role of chromatin organisation in transcription factor target search and damage site recruitment of DNA repair factor machinery. To achieve this I have developed fluorescence microscopy methods to monitor changes in chromatin structure with submicron resolution. Only with this technology can I determine how chromatin dynamics maintain genome integrity or induce disease.
Related publications (9)
A dominant-negative SOX18 mutant disrupts multiple regulatory layers essential to transcription factor activity.
Alex J McCann, Jieqiong Lou, Mehdi Moustaqil, Matthew S Graus, Ailisa Blum, Frank Fontaine, Hui Liu, Winnie Luu, Paulina Rudolffi-Soto, Peter Koopman, Emma Sierecki, Yann Gambin, Frédéric A Meunier, Zhe Liu, Elizabeth Hinde, Mathias Francois
Few genetically dominant mutations involved in human disease have been fully explained at the molecular level. In cases where the ..
Super-resolution microscopy reveals the arrangement of inner membrane protein complexes in mammalian mitochondria
Catherine S Palmer, Jieqiong Lou, Betty Kouskousis, Elvis Pandzic, Alexander J Anderson, Yilin Kang, Elizabeth Hinde, Diana Stojanovski
The mitochondrial inner membrane is a protein-rich environment containing large multimeric complexes, including complexes of the m..
Nanophotonics enhanced coverslip for phase imaging in biology
Lukas Wesemann, Jon Rickett, Jingchao Song, Jieqiong Lou, Elizabeth Hinde, Timothy J Davis, Ann Roberts
The ability to visualise transparent objects such as live cells is central to understanding biological processes. Here we experime..
Perilipin 5 S155 phosphorylation by PKA is required for the control of hepatic lipid metabolism and glycemic control
Stacey N Keenan, William De Nardo, Jieqiong Lou, Ralf B Schittenhelm, Magdalene K Montgomery, James G Granneman, Elizabeth Hinde, Matthew J Watt
Perilipin 5 (PLIN5) is a lipid-droplet-associated protein that coordinates intracellular lipolysis in highly oxidative tissues and..
Spatiotemporal dynamics of 53BP1 dimer recruitment to a DNA double strand break
Jieqiong Lou, David G Priest, Ashleigh Solano, Adele Kerjouan, Elizabeth Hinde
Tumor suppressor p53-binding protein 1 (53BP1) is a DNA repair protein essential for the detection, assessment, and resolution of ..
Quantifying nuclear wide chromatin compaction by phasor analysis of histone Forster resonance energy transfer (FRET) in frequency domain fluorescence lifetime imaging microscopy (FLIM) data
Zhen Liang, Jieqiong Lou, Lorenzo Scipioni, Enrico Gratton, Elizabeth Hinde
The nanometer spacing between nucleosomes throughout global chromatin organisation modulates local DNA template access, and throug..
Nuclear bodies formed by polyQ-ataxin-1 protein are liquid RNA/protein droplets with tunable dynamics
Sunyuan Zhang, Elizabeth Hinde, Molly Parkyn Schneider, David A Jans, Marie A Bogoyevitch
A mutant form of the ataxin-1 protein with an expanded polyglutamine (polyQ) tract is the underlying cause of the inherited neurod..
Phasor histone FLIM-FRET microscopy quantifies spatiotemporal rearrangement of chromatin architecture during the DNA damage response
Jieqiong Lou, Lorenzo Scipioni, Belinda K Wright, Tara K Bartolec, Jessie Zhang, V Pragathi Masamsetti, Katharina Gaus, Enrico Gratton, Anthony J Cesare, Elizabeth Hinde
To investigate how chromatin architecture is spatiotemporally organized at a double-strand break (DSB) repair locus, we establishe..