Using chemistry to illuminate sulfoglycolysis, a major organosulfur pathway
Grant number: DP180101957 | Funding period: 2018 - 2021
This project aims to develop a detailed molecular description of the sulfoglycolysis pathway, a major pathway involved in cycling an abundant sulfolipid. The project will use an integrated chemical, biochemical and structural approach to illuminate how sulfoglycolysis degrades sulfolipid to access its elemental and energy constituents. Expected outcomes include an advanced understanding of the biosulfur cycle, the development of new chemical approaches to manipulate sulfur cycling for agricultural and biotechnology applications, and deepened ties to leading international researchers. Potential benefits include new strategies to reduce dependence on agricultural fertilisers, promote gut wellb..View full description
Related publications (17)
Molecular Basis of Sulfosugar Selectivity in Sulfoglycolysis
Mahima Sharma, Palika Abayakoon, Ruwan Epa, Yi Jin, James P Lingford, Tomohiro Shimada, Masahiro Nakano, Janice W-Y Mui, Akira Ishihama, Ethan D Goddard-Borger, Gideon J Davies, Spencer J Williams
The sulfosugar sulfoquinovose (SQ) is produced by essentially all photosynthetic organisms on Earth and is metabolized by bacteria..
Candida albicans steryl 6-O-acyl-alpha-d-mannosides agonize signalling through Mincle
Tram Nguyen, Yuki Hosono, Takashi Shimizu, Sho Yamasaki, Spencer J Williams
The C-type lectin receptor Mincle binds Candida albicans and has been implicated in its pathobiology, but the molecular effectors ..
Structure of human endo-alpha-1,2-mannosidase (MANEA), an antiviral host-glycosylation target
Lukasz F Sobala, Pearl Z Fernandes, Zalihe Hakki, Andrew J Thompson, Jonathon D Howe, Michelle Hill, Nicole Zitzmann, Scott Davies, Zania Stamataki, Terry D Butters, Dominic S Alonzi, Spencer J Williams, Gideon J Davies
Mammalian protein N-linked glycosylation is critical for glycoprotein folding, quality control, trafficking, recognition, and func..
Cholesteryl 6-O-acyl-alpha-glucosides from diverseHelicobacterspp. signal through the C-type lectin receptor Mincle
Dylan GM Smith, Emi Ito, Sho Yamasaki, Spencer J Williams
Helicobacter spp. are Gram-negative bacteria that cause a spectrum of disease in the gut, biliary tree and liver. Many Helicobacte..
A Sulfoglycolytic Entner-Doudoroff Pathway in Rhizobium leguminosarum bv. trifolii SRDI565
Jinling Li, Ruwan Epa, Nichollas E Scott, Dominik Skoneczny, Mahima Sharma, Alexander JD Snow, James P Lingford, Ethan D Goddard-Borger, Gideon J Davies, Malcolm J McConville, Spencer J Williams
Rhizobia are nitrogen-fixing bacteria that engage in symbiotic relationships with plant hosts but can also persist as free-living ..
An Epoxide Intermediate in Glycosidase Catalysis
Lukasz F Sobala, Gaetano Speciale, Sha Zhu, Lluis Raich, Natalia Sannikova, Andrew J Thompson, Zalihe Hakki, Dan Lu, Saeideh Shamsi Kazem Abadi, Andrew R Lewis, Victor Rojas-Cervellera, Ganeko Bernardo-Seisdedos, Yongmin Zhang, Oscar Millet, Jesus Jimenez-Barbero, Andrew J Bennet, Matthieu Sollogoub, Carme Rovira, Gideon J Davies, Spencer J Williams
Retaining glycoside hydrolases cleave their substrates through stereochemical retention at the anomeric position. Typically, this ..
Dynamic Structural Changes Accompany the Production of Dihydroxypropanesulfonate by Sulfolactaldehyde Reductase
Mahima Sharma, Palika Abayakoon, James P Lingford, Ruwan Epa, Alan John, Yi Jin, Ethan D Goddard-Borger, Gideon J Davies, Spencer J Williams
2,3-Dihydroxypropanesulfonate (DHPS) is a major sulfur species in the biosphere. One important route for the production of DHPS is..
Concise synthesis of sulfoquinovose and sulfoquinovosyl diacylglycerides, and development of a fluorogenic substrate for sulfoquinovosidases
Yunyang Zhang, Janice W-Y Mui, Thimali Arumaperuma, James P Lingford, Ethan D Goddard-Borger, Jonathan M White, Spencer J Williams
The sulfolipid sulfoquinovosyl diacylglycerol (SQDG) and its headgroup, the sulfosugar sulfoquinovose (SQ), are estimated to harbo..
A sulfoglycolytic Entner-Doudoroff pathway in Rhizobium leguminosarum bv. trifolii SRDI565
Jinling Li, Ruwan Epa, Nichollas Scott, Dominic Skoneczny, Mahima Sharma, Alexander JD Snow, James Lingford, Ethan Goddard-Borger, Gideon Davies, Malcolm McConville, Spencer Williams
Rhizobia are nitrogen fixing bacteria that engage in symbiotic relationships with plant hosts but can also persist as free-living ..
Distortion of mannoimidazole supports a B-2,B-5 boat transition state for the family GH125 alpha-1,6-mannosidase from Clostridium perfringens
Alexandra Males, Gaetano Speciale, Spencer J Williams, Gideon J Davies
Enzyme transition-state mimics can act as powerful inhibitors and allow structural studies that report on the conformation of the ..
A Family of Dual-Activity Glycosyltransferase-Phosphorylases Mediates Mannogen Turnover and Virulence in Leishmania Parasites
M Fleur Sernee, Julie E Ralton, Tracy L Nero, Lukasz F Sobala, Joachim Kloehn, Marcel A Vieira-Lara, Simon A Cobbold, Lauren Stanton, Douglas EV Pires, Eric Hanssen, Alexandra Males, Tom Ward, Laurence M Bastidas, Phillip L van der Peet, Michael W Parker, David B Ascher, Spencer J Williams, Gideon J Davies, Malcolm J McConville
Parasitic protists belonging to the genus Leishmania synthesize the non-canonical carbohydrate reserve, mannogen, which is compose..
Eucalyptus subgenus Eucalyptus (Myrtaceae) trees are abundant sources of medicinal pinocembrin and related methylated flavanones
Jason QD Goodger, Samiddhi L Senaratne, Phillip van der Peet, Rhys Browning, Spencer J Williams, Dean Nicolle, Ian E Woodrow
Plant-derived flavanones such as pinocembrin and related methylated forms are of interest for their potential medicinal use. Comme..
Comprehensive Synthesis of Substrates, Intermediates, and Products of the Sulfoglycolytic Embden-Meyerhoff-Parnas Pathway
Palika Abayakoon, Ruwan Epa, Marija Petricevic, Christopher Bengt, Janice W-Y Mui, Phillip L van der Peet, Yunyang Zhang, James P Lingford, Jonathan M White, Ethan D Goddard-Borger, Spencer J Williams
Sulfoglycolysis is a metabolic pathway dedicated to the catabolism of the sulfosugar sulfoquinovose (SQ) into smaller organosulfur..
Structural and Biochemical Insights into the Function and Evolution of Sulfoquinovosidases
Palika Abayakoon, Yi Jin, James P Lingford, Marija Petricevic, Alan John, Eileen Ryan, Janice Wai-Ying Mui, Douglas E Pires, David B Ascher, Gideon J Davies, Ethan D Goddard-Borger, Spencer J Williams
An estimated 10 billion tonnes of sulfoquinovose (SQ) are produced and degraded each year. Prokaryotic sulfoglycolytic pathways ca..