Nuclear architecture in a living cell facilitates navigation of the genome.
Grant number: DP180101387 | Funding period: 2018 - 2020
This project aims to investigate the role of nuclear architecture in regulating genome function by development of a new microscopy method to quantify the diffusive route of fluorescent proteins in live cells. The anticipated outcomes of this project include an insight into how chromatin dynamics facilitate DNA target search and an analytical tool for cell biologists to probe how genomes work in their natural environment (the cell nucleus).
Related publications (11)
Radial pair correlation of molecular brightness fluctuations maps protein diffusion as a function of oligomeric state within live-cell nuclear architecture
Ashleigh Solano, Jiediong Lou, Lorenzo Scipioni, Enrico Gratton, Elizabeth Hinda
Nuclear proteins can modulate their DNA binding activity and the exploration volume available during DNA target search by self-ass..
Heterochromatic repeat clustering imposes a physical barrier on homologous recombination to prevent chromosomal translocations
Ioanna Mitrentsi, Jieqiong Lou, Adele Kerjouan, John Verigos, Bernardo Reina-San-Martin, Elizabeth Hinde, Evi Soutoglou
Mouse pericentromeric DNA is composed of tandem major satellite repeats, which are heterochromatinized and cluster together to for..
Fluorescence lifetime imaging microscopy (FLIM): a non-traditional approach to study host-microbial symbioses
Pranali Deore, Iromi Wanigasuriya, Sarah Jane Tsang Min Ching, Douglas R Brumley, Madeleine JH van Oppen, Linda L Blackall, Elizabeth Hinde
Corals and their photosynthetic endosymbiotic algae (Symbiodiniaceae) produce a strong autofluorescent signal that spans the visib..
Phasor Histone FLIM-FRET Microscopy Maps Nuclear-Wide Nanoscale Chromatin Architecture With Respect to Genetically Induced DNA Double-Strand Breaks
Jieqiong Lou, Ashleigh Solano, Zhen Liang, Elizabeth Hinde
A DNA double-strand break (DSB) takes place in the context of chromatin, and there is increasing evidence for chromatin structure ..
A dominant-negative SOX18 mutant disrupts multiple regulatory layers essential to transcription factor activity
Alex J McCann, Jieqiong Lou, Mehdi Moustaqil, Matthew S Graus, Ailisa Blum, Frank Fontaine, Hui Liu, Winnie Luu, Paulina Rudolffi-Soto, Peter Koopman, Emma Sierecki, Yann Gambin, Frederic A Meunier, Zhe Liu, Elizabeth Hinde, Mathias Francois
Few genetically dominant mutations involved in human disease have been fully explained at the molecular level. In cases where the ..
Super-resolution microscopy reveals the arrangement of inner membrane protein complexes in mammalian mitochondria
Catherine S Palmer, Jieqiong Lou, Betty Kouskousis, Elvis Pandzic, Alexander J Anderson, Yilin Kang, Elizabeth Hinde, Diana Stojanovski
The mitochondrial inner membrane is a protein-rich environment containing large multimeric complexes, including complexes of the m..
Nanophotonics enhanced coverslip for phase imaging in biology
Lukas Wesemann, Jon Rickett, Jingchao Song, Jieqiong Lou, Elizabeth Hinde, Timothy J Davis, Ann Roberts
The ability to visualise transparent objects such as live cells is central to understanding biological processes. Here we experime..
Spatiotemporal dynamics of 53BP1 dimer recruitment to a DNA double strand break
Jieqiong Lou, David G Priest, Ashleigh Solano, Adele Kerjouan, Elizabeth Hinde
Tumor suppressor p53-binding protein 1 (53BP1) is a DNA repair protein essential for the detection, assessment, and resolution of ..
Quantifying nuclear wide chromatin compaction by phasor analysis of histone Forster resonance energy transfer (FRET) in frequency domain fluorescence lifetime imaging microscopy (FLIM) data
Zhen Liang, Jieqiong Lou, Lorenzo Scipioni, Enrico Gratton, Elizabeth Hinde
The nanometer spacing between nucleosomes throughout global chromatin organisation modulates local DNA template access, and throug..
Phasor histone FLIM-FRET microscopy quantifies spatiotemporal rearrangement of chromatin architecture during the DNA damage response
Jieqiong Lou, Lorenzo Scipioni, Belinda K Wright, Tara K Bartolec, Jessie Zhang, V Pragathi Masamsetti, Katharina Gaus, Enrico Gratton, Anthony J Cesare, Elizabeth Hinde
To investigate how chromatin architecture is spatiotemporally organized at a double-strand break (DSB) repair locus, we establishe..