Nuclear architecture in a living cell facilitates navigation of the genome.
Grant number: DP180101387 | Funding period: 2018 - 2020
This project aims to investigate the role of nuclear architecture in regulating genome function by development of a new microscopy method to quantify the diffusive route of fluorescent proteins in live cells. The anticipated outcomes of this project include an insight into how chromatin dynamics facilitate DNA target search and an analytical tool for cell biologists to probe how genomes work in their natural environment (the cell nucleus).
Related publications (3)
Spatiotemporal dynamics of 53BP1 dimer recruitment to a DNA double strand break
Jieqiong Lou, David G Priest, Ashleigh Solano, Adele Kerjouan, Elizabeth Hinde
Tumor suppressor p53-binding protein 1 (53BP1) is a DNA repair protein essential for the detection, assessment, and resolution of ..
Phasor histone FLIM-FRET microscopy quantifies spatiotemporal rearrangement of chromatin architecture during the DNA damage response
Jieqiong Lou, Lorenzo Scipioni, Belinda K Wright, Tara K Bartolec, Jessie Zhang, V Pragathi Masamsetti, Katharina Gaus, Enrico Gratton, Anthony J Cesare, Elizabeth Hinde