Nuclear architecture in a living cell facilitates navigation of the genome.

Radial pair correlation of molecular brightness fluctuations maps protein diffusion as a function of oligomeric state within live-cell nuclear architecture

Ashleigh Solano, Jiediong Lou, Lorenzo Scipioni, Enrico Gratton, Elizabeth Hinda

2022-06-07

Nuclear proteins can modulate their DNA binding activity and the exploration volume available during DNA target search by self-ass..

Heterochromatic repeat clustering imposes a physical barrier on homologous recombination to prevent chromosomal translocations

Ioanna Mitrentsi, Jieqiong Lou, Adele Kerjouan, John Verigos, Bernardo Reina-San-Martin, Elizabeth Hinde, Evi Soutoglou

2022-06-02

Mouse pericentromeric DNA is composed of tandem major satellite repeats, which are heterochromatinized and cluster together to for..

Fluorescence lifetime imaging microscopy (FLIM): a non-traditional approach to study host-microbial symbioses

Pranali Deore, Iromi Wanigasuriya, Sarah Jane Tsang Min Ching, Douglas R Brumley, Madeleine JH van Oppen, Linda L Blackall, Elizabeth Hinde

2022-04-14

Corals and their photosynthetic endosymbiotic algae (Symbiodiniaceae) produce a strong autofluorescent signal that spans the visib..

Phasor Histone FLIM-FRET Microscopy Maps Nuclear-Wide Nanoscale Chromatin Architecture With Respect to Genetically Induced DNA Double-Strand Breaks

Jieqiong Lou, Ashleigh Solano, Zhen Liang, Elizabeth Hinde

2021-12-10

A DNA double-strand break (DSB) takes place in the context of chromatin, and there is increasing evidence for chromatin structure ..

A dominant-negative SOX18 mutant disrupts multiple regulatory layers essential to transcription factor activity

Alex J McCann, Jieqiong Lou, Mehdi Moustaqil, Matthew S Graus, Ailisa Blum, Frank Fontaine, Hui Liu, Winnie Luu, Paulina Rudolffi-Soto, Peter Koopman, Emma Sierecki, Yann Gambin, Frederic A Meunier, Zhe Liu, Elizabeth Hinde, Mathias Francois

2021-09-27

Few genetically dominant mutations involved in human disease have been fully explained at the molecular level. In cases where the ..

Super-resolution microscopy reveals the arrangement of inner membrane protein complexes in mammalian mitochondria

Catherine S Palmer, Jieqiong Lou, Betty Kouskousis, Elvis Pandzic, Alexander J Anderson, Yilin Kang, Elizabeth Hinde, Diana Stojanovski

2021-07-01

The mitochondrial inner membrane is a protein-rich environment containing large multimeric complexes, including complexes of the m..

Live cell dynamics of the NF-Y transcription factor

David G Priest, Andrea Bernardini, Jieqiong Lou, Roberto Mantovani, Elizabeth Hinde

2021-05-26

Transcription factors (TFs) are core players in the control of gene expression, evolutionarily selected to recognise a subset of s..

Nanophotonics enhanced coverslip for phase imaging in biology

Lukas Wesemann, Jon Rickett, Jingchao Song, Jieqiong Lou, Elizabeth Hinde, Timothy J Davis, Ann Roberts

2021-05-08

The ability to visualise transparent objects such as live cells is central to understanding biological processes. Here we experime..

Spatiotemporal dynamics of 53BP1 dimer recruitment to a DNA double strand break

Jieqiong Lou, David G Priest, Ashleigh Solano, Adele Kerjouan, Elizabeth Hinde

2020-11-13

Tumor suppressor p53-binding protein 1 (53BP1) is a DNA repair protein essential for the detection, assessment, and resolution of ..

Quantifying nuclear wide chromatin compaction by phasor analysis of histone Forster resonance energy transfer (FRET) in frequency domain fluorescence lifetime imaging microscopy (FLIM) data

Zhen Liang, Jieqiong Lou, Lorenzo Scipioni, Enrico Gratton, Elizabeth Hinde

2020-06-01

The nanometer spacing between nucleosomes throughout global chromatin organisation modulates local DNA template access, and throug..

Phasor histone FLIM-FRET microscopy quantifies spatiotemporal rearrangement of chromatin architecture during the DNA damage response

Jieqiong Lou, Lorenzo Scipioni, Belinda K Wright, Tara K Bartolec, Jessie Zhang, V Pragathi Masamsetti, Katharina Gaus, Enrico Gratton, Anthony J Cesare, Elizabeth Hinde

2019-04-09

To investigate how chromatin architecture is spatiotemporally organized at a double-strand break (DSB) repair locus, we establishe..