DEFINING THE ROLE OF ZINC AT THE HOST-PNEUMOCOCCAL INTERFACE

Structural and biochemical characterization of Acinetobacter baumannii ZnuA

Saleh Alquethamy, Katherine Ganio, Zhenyao Luo, Sheikh I Hossain, Andrew J Hayes, Thomas Ve, Mark R Davies, Evelyne Deplazes, Bostjan Kobe, Christopher A McDevitt

2022-06-01

Acinetobacter baumannii is a Gram-negative nosocomial pathogen associated with significant disease. Crucial to the survival and pa..

The Impact of Chromate on Pseudomonas aeruginosa Molybdenum Homeostasis

Eve A Maunders, Dalton HY Ngu, Katherine Ganio, Sheikh I Hossain, Bryan YJ Lim, Michael G Leeming, Zhenyao Luo, Aimee Tan, Evelyne Deplazes, Bostjan Kobe, Christopher A McDevitt

2022-05-24

Acquisition of the trace-element molybdenum via the high-affinity ATP-binding cassette permease ModABC is essential for Pseudomona..

The Role of ZntA in Klebsiella pneumoniae Zinc Homeostasis

Eve A Maunders, Katherine Ganio, Andrew J Hayes, Stephanie L Neville, Mark R Davies, Richard A Strugnell, Christopher A McDevitt, Aimee Tan

2022-02-01

Klebsiella pneumoniae is an opportunistic Gram-negative pathogen that is a leading cause of healthcare-associated infections, incl..

Dysregulation of Streptococcus pneumoniae zinc homeostasis breaks ampicillin resistance in a pneumonia infection model

Erin B Brazel, Aimee Tan, Stephanie L Neville, Amy R Iverson, Saumya R Udagedara, Bliss A Cunningham, Mwilye Sikanyika, David MP De Oliveira, Bernhard Keller, Lisa Bohlmann, Ibrahim M El-Deeb, Katherine Ganio, Bart A Eijkelkamp, Alastair G McEwan, Mark von Itzstein, Megan J Maher, Mark J Walker, Jason W Rosch, Christopher A McDevitt

2022-01-11

Streptococcus pneumoniae is the primary cause of community-acquired bacterial pneumonia with rates of penicillin and multidrug-res..

Conformation of the Solute-Binding Protein AdcAII Influences Zinc Uptake in Streptococcus pneumoniae

Marina L Zupan, Zhenyao Luo, Katherine Ganio, Victoria G Pederick, Stephanie L Neville, Evelyne Deplazes, Bostjan Kobe, Christopher A McDevitt

2021-08-19

Streptococcus pneumoniae scavenges essential zinc ions from the host during colonization and infection. This is achieved by the AT..

The structural basis of bacterial manganese import

Stephanie L Neville, Jennie Sjoehamn, Jacinta A Watts, Hugo MacDermott-Opeskin, Stephen J Fairweather, Katherine Ganio, Alex Carey Hulyer, Aaron P McGrath, Andrew J Hayes, Tess R Malcolm, Mark R Davies, Norimichi Nomura, So Iwata, Megan L O'Mara, Megan J Maher, Christopher A McDevitt

2021-08-01

Metal ions are essential for all forms of life. In prokaryotes, ATP-binding cassette (ABC) permeases serve as the primary import p..

A Trap-Door Mechanism for Zinc Acquisition by Streptococcus pneumoniae AdcA

Zhenyao Luo, Jacqueline R Morey, Evelyne Deplazes, Alina Motygullina, Aimee Tan, Katherine Ganio, Stephanie L Neville, Nikolaos Eleftheriadis, Michael Isselstein, Victoria G Pederick, James C Paton, Thorben Cordes, Jeffrey R Harmer, Bostjan Kobe, Christopher A McDevitt

2021-01-01

Zinc is an essential element in all domains of life. Nonetheless, how prokaryotes achieve selective acquisition of zinc from the e..

Cadmium stress dictates central carbon flux and alters membrane composition in Streptococcus pneumoniae

Stephanie L Neville, Bart A Eijkelkamp, Amber Lothian, James C Paton, Blaine R Roberts, Jason W Rosch, Christopher A McDevitt

2020-12-19

Metal ion homeostasis is essential for all forms of life. However, the breadth of intracellular impacts that arise upon dysregulat..

Disruption of Phosphate Homeostasis Sensitizes Staphylococcus aureus to Nutritional Immunity

Jessica L Kelliher, Erin B Brazel, Jana N Radin, Eliot S Joya, Paola K Parraga Solorzano, Stephanie L Neville, Christopher A McDevitt, Thomas E Kehl-Fie

2020-05-01

To control infection, mammals actively withhold essential nutrients, including the transition metal manganese, by a process termed..

Intracellular Accumulation of Staphylopine Can Sensitize Staphylococcus aureus to Host-Imposed Zinc Starvation by Chelation-Independent Toxicity

Kyle P Grim, Jana N Radin, Paola K Parraga Solorzano, Jacqueline R Morey, Katie A Frye, Katherine Ganio, Stephanie L Neville, Christopher A McDevitt, Thomas E Kehl-Fie

2020-05-01

The host restricts the availability of zinc to prevent infection. To overcome this defense, Staphylococcus aureus and Pseudomonas ..

Structure and Metal Binding Properties of Chlamydia trachomatis YtgA

Zhenyao Luo, Stephanie L Neville, Rebecca Campbell, Jacqueline R Morey, Shruti Menon, Mark Thomas, Bart A Eijkelkamp, Miranda P Ween, Wilhelmina M Huston, Bostjan Kobe, Christopher A McDevitt

2020-01-01

The obligate intracellular pathogen Chlamydia trachomatis is a globally significant cause of sexually transmitted bacterial infect..

The Role of Zinc Efflux during Acinetobacter baumannii Infection

Saleh F Alquethamy, Felise G Adams, Varsha Naidu, Marian Khorvash, Victoria G Pederick, Maoge Zang, James C Paton, Ian T Paulsen, Karl A Hassan, Amy K Cain, Christopher A McDevitt, Bart A Eijkelkamp

2020-01-01

Acinetobacter baumannii is a ubiquitous Gram-negative bacterium, that is associated with significant disease in immunocompromised ..

Dietary zinc and the control of Streptococcus pneumoniae infection

Bart A Eijkelkamp, Jacqueline R Morey, Stephanie L Neville, Aimee Tan, Victoria G Pederick, Nerida Cole, Prashina P Singh, Cheryl-Lynn Y Ong, Raquel Gonzalez de Vega, David Clases, Bliss A Cunningham, Catherine E Hughes, Iain Comerford, Erin B Brazel, Jonathan J Whittall, Charles D Plumptre, Shaun R McColl, James C Paton, Alastair G McEwan, Philip A Doble

2019-08-01

Human zinc deficiency increases susceptibility to bacterial infection. Although zinc supplementation therapies can reduce the impa..

Conformational and dynamic plasticity in substrate-binding proteins underlies selective transport in ABC importers

Marijn de Boer, Giorgos Gouridis, Ruslan Vietrov, Stephanie L Begg, Gea K Schuurman-Wolters, Florence Husada, Nikolaos Eleftheriadis, Bert Poolman, Christopher A McDevit, Thorben Cordes

2019-03-22

Substrate-binding proteins (SBPs) are associated with ATP-binding cassette importers and switch from an open to a closed conformat..

The Role of the CopA Copper Efflux System in Acinetobacter baumannii Virulence

Saleh F Alquethamy, Marjan Khorvash, Victoria G Pederick, Jonathan J Whittall, James C Paton, Ian T Paulsen, Karl A Hassan, Christopher A McDevitt, Bart A Eijkelkamp

2019-02-01

Acinetobacter baumannii has emerged as one of the leading causative agents of nosocomial infections. Due to its high level of intr..

Identification of Novel Acinetobacter baumannii Host Fatty Acid Stress Adaptation Strategies

Jhih-Hang Jiang, Karl A Hassan, Stephanie L Begg, Thusitha WT Rupasinghe, Varsha Naidu, Victoria G Pederick, Marjan Khorvash, Jonathan J Whittall, James C Paton, Ian T Paulsen, Christopher A McDevitt, Anton Y Peleg, Bart A Eijkelkamp

2019-01-01

Free fatty acids hold important immune-modulatory roles during infection. However, the host's long-chain polyunsaturated fatty aci..

Synergy between Nutritional Immunity and Independent Host Defenses Contributes to the Importance of the MntABC Manganese Transporter during Staphylococcus aureus Infection

Jana N Radin, Jamie Zhu, Erin B Brazel, Christopher A McDevitt, Thomas E Kehl-Fie

2019-01-01

During infection, the host utilizes a diverse array of processes to combat invaders, including the restriction of availability of ..

A Liposomal Platform for Sensing of Extracellular Analytes Near Cells

Xiaozhou Zhang, Sabrina Heng, Jinxin Pei, Jacqueline R Morey, Christopher A McDevitt, Andrew D Abell

2018-12-01

Cell-permeable fluorescent chemosensors (calcein, monochlorobimane, and a recently reported spiropyran-based sensor SP2) have been..

Antimicrobial Susceptibility of Escherichia coli and Salmonella spp. Isolates From Healthy Pigs in Australia: Results of a Pilot National Survey

Amanda K Kidsley, Sam Abraham, Jan M Bell, Mark O'Dea, Tanya J Laird, David Jordan, Pat Mitchell, Christopher A McDevitt, Darren J Trott

2018-07-09

This study investigated the frequency of antimicrobial non-susceptibility (defined as the frequency of isolates with minimum inhib..

Structural characterisation of the HT3 motif of the polyhistidine triad protein D from Streptococcus pneumoniae

Zhenyao Luo, Victoria G Pederick, James C Paton, Christopher A McDevitt, Bostjan Kobe

2018-07-01

The bacterium Streptococcus pneumoniae (the pneumococcus) is a major human pathogen that requires Zn2+ for its survival and virule..

Arachidonic Acid Stress Impacts Pneumococcal Fatty Acid Homeostasis

Bart A Eijkelkamp, Stephanie L Begg, Victoria G Pederick, Claudia Trapetti, Melissa K Gregory, Jonathan J Whittall, James C Paton, Christopher A McDevitt

2018-05-11

Free fatty acids hold dual roles during infection, serving to modulate the host immune response while also functioning directly as..

Biotin-mediated growth and gene expression in Staphylococcus aureus is highly responsive to environmental biotin

Jiulia Satiaputra, Bart A Eijkelkamp, Christopher A McDevitt, Keith E Shearwin, Grant W Booker, Steven W Polyak

2018-04-01

Biotin (Vitamin B7) is a critical enzyme co-factor in metabolic pathways important for bacterial survival. Biotin is obtained eith..

An optimized SEC-SAXS system enabling high X-ray dose for rapid SAXS assessment with correlated UV measurements for biomolecular structure analysis

Timothy M Ryan, Jill Trewhella, James M Murphy, Jeremy R Keown, Lachlan Casey, F Grant Pearce, David C Goldstone, Kelan Chen, Zhenyao Luo, Bostjan Kobe, Christopher A McDevitt, Serena A Watkin, Adrian M Hawley, Stephen T Mudie, Vesna Samardzic Boban, Nigel Kirby

2018-02-01

A new optimized size exclusion chromatography small-angle X-ray scattering (SEC-SAXS) system for biomolecular SAXS at the Australi..

Genome-Wide Mutagenesis of Dengue Virus Reveals Plasticity of the NS1 Protein and Enables Generation of Infectious Tagged Reporter Viruses

Nicholas S Eyre, Stephen M Johnson, Auda A Eltahla, Maria Aloi, Amanda L Aloia, Christopher A McDevitt, Rowena A Bull, Michael R Beard

2017-12-01

Dengue virus (DENV) is a major global pathogen that causes significant morbidity and mortality in tropical and subtropical areas w..

The Metallophore Staphylopine Enables Staphylococcus aureus To Compete with the Host for Zinc and Overcome Nutritional Immunity

Kyle P Grim, Brian San Francisco, Jana N Radin, Erin B Brazel, Jessica L Kelliher, Paola K Parraga Solorzano, Philip C Kim, Christopher A McDevitt, Thomas E Kehl-Fie

2017-09-01

During infection, the host sequesters essential nutrients, such as zinc, to combat invading microbes. Despite the ability of the i..

The zinc efflux activator SczA protects Streptococcus pneumoniae serotype 2 D39 from intracellular zinc toxicity

Julia E Martin, Katherine A Edmonds, Kevin E Bruce, Gregory C Campanello, Bart A Eijkelkamp, Erin B Brazel, Christopher A McDevitt, Malcolm E Winkler, David P Giedroc

2017-05-01

Zinc is an essential trace element that serves as a catalytic cofactor in metalloenzymes and a structural element in proteins invo..

Zinc stress induces copper depletion in Acinetobacter baumannii

Karl A Hassan, Victoria G Pederick, Liam DH Elbourne, Ian T Paulsen, James C Paton, Christopher A McDevitt, Bart A Eijkelkamp

2017-03-11

BACKGROUND: The first row transition metal ions zinc and copper are essential to the survival of many organisms, although in exces..

Autoinducer 2 Signaling via the Phosphotransferase FruA Drives Galactose Utilization by Streptococcus pneumoniae, Resulting in Hypervirulence

Claudia Trappetti, Lauren J McAllister, Austen Chen, Hui Wang, Adrienne W Paton, Marco R Oggioni, Christopher A McDevitt, James C Paton

2017-01-01

UNLABELLED: Communication between bacterial cells is crucial for the coordination of diverse cellular processes that facilitate en..

Metal ion Toxicity and Oxidative Stress in Streptococcus Pneumoniae

CA McDevitt, SL Begg, JC Paton

2016-08-12

Streptococcus pneumoniae is one of the world's foremost bacterial pathogens. Primarily residing asymptomatically in the human naso..

The First Histidine Triad Motif of PhtD Is Critical for Zinc Homeostasis in Streptococcus pneumoniae

Bart A Eijkelkamp, Victoria G Pederick, Charles D Plumptre, Richard M Harvey, Catherine E Hughes, James C Paton, Christopher A McDevitt

2016-02-01

Streptococcus pneumoniae is the world's foremost human pathogen. Acquisition of the first row transition metal ion zinc is essenti..

Characterizing the conformational dynamics of metal-free PsaA using molecular dynamics simulations and electron paramagnetic resonance spectroscopy

Evelyne Deplazes, Stephanie L Begg, Jessica H van Wonderen, Rebecca Campbell, Bostjan Kobe, James C Paton, Fraser MacMillan, Christopher A McDevitt, Megan L O'Mara

2015-12-01

Prokaryotic metal-ion receptor proteins, or solute-binding proteins, facilitate the acquisition of metal ions from the extracellul..

Heterogeneous nucleation is required for crystallization of the ZnuA domain of pneumococcal AdcA

Zhenyao Luo, Jacqueline R Morey, Christopher A McDevitt, Bostjan Kobe

2015-12-01

Zn(2+) is an essential nutrient for all known forms of life. In the major human pathogen Streptococcus pneumoniae, the acquisition..