MOLECULAR INSIGHTS INTO BACTERIAL METAL ION HOMEOSTASIS AND TOXICITY
Grant number: DP170102102 | Funding period: 2017 - 2020
This project aims to measure bacterial cellular metal concentrations, elucidate mechanisms cells use to adapt to changing extracellular metal concentrations, and reveal the molecular targets of metal toxicity. Metal ions are essential to all forms of life, and half of all proteins use metal ions for cellular chemical processes. However, how cells precisely balance sufficient metal ions for essential cellular chemistry without accumulating a toxic excess (metal homeostasis) is poorly understood. Discovering the roles of metal ions in bacterial cells will be key to defining the chemical biology of living systems and will provide information essential to understanding how microbes adapt to chan..View full description
Related publications (20)
Cadmium stress dictates central carbon flux and alters membrane composition in Streptococcus pneumoniae
Stephanie L Neville, Bart A Eijkelkamp, Amber Lothian, James C Paton, Blaine R Roberts, Jason W Rosch, Christopher A McDevitt
Metal ion homeostasis is essential for all forms of life. However, the breadth of intracellular impacts that arise upon dysregulat..
Structural and functional characterizations of the C-terminal domains of CzcD proteins
Saumya R Udagedara, Daniel M La Porta, Christian Spehar, Ghruta Purohit, Matthew JA Hein, Monique E Fatmous, G Patricia Casas Garcia, Katherine Ganio, Christopher A McDevitt, Megan J Maher
Zinc is a potent antimicrobial component of the innate immune response at the host-pathogen interface. Bacteria subvert or resist ..
Disruption of Phosphate Homeostasis Sensitizes Staphylococcus aureus to Nutritional Immunity
Jessica L Kelliher, Erin B Brazel, Jana N Radin, Eliot S Joya, Paola K Parraga Solorzano, Stephanie L Neville, Christopher A McDevitt, Thomas E Kehl-Fie
To control infection, mammals actively withhold essential nutrients, including the transition metal manganese, by a process termed..
Experimental Evolution In Vivo To Identify Selective Pressures during Pneumococcal Colonization
Vaughn S Cooper, Erin Honsa, Hannah Rowe, Christopher Deitrick, Amy R Iverson, Jonathan J Whittall, Stephanie L Neville, Christopher A McDevitt, Colin Kietzman, Jason W Rosch
Experimental evolution is a powerful technique to understand how populations evolve from selective pressures imparted by the surro..
Intracellular Accumulation of Staphylopine Can Sensitize Staphylococcus aureus to Host-Imposed Zinc Starvation by Chelation-Independent Toxicity
Kyle P Grim, Jana N Radin, Paola K Parraga Solorzano, Jacqueline R Morey, Katie A Frye, Katherine Ganio, Stephanie L Neville, Christopher A McDevitt, Thomas E Kehl-Fie
The host restricts the availability of zinc to prevent infection. To overcome this defense, Staphylococcus aureus and Pseudomonas ..
Structure and Metal Binding Properties of Chlamydia trachomatis YtgA
Zhenyao Luo, Stephanie L Neville, Rebecca Campbell, Jacqueline R Morey, Shruti Menon, Mark Thomas, Bart A Eijkelkamp, Miranda P Ween, Wilhelmina M Huston, Bostjan Kobe, Christopher A McDevitt
The obligate intracellular pathogen Chlamydia trachomatis is a globally significant cause of sexually transmitted bacterial infect..
The Role of Zinc Efflux during Acinetobacter baumannii Infection
Saleh F Alquethamy, Felise G Adams, Varsha Naidu, Marian Khorvash, Victoria G Pederick, Maoge Zang, James C Paton, Ian T Paulsen, Karl A Hassan, Amy K Cain, Christopher A McDevitt, Bart A Eijkelkamp
Acinetobacter baumannii is a ubiquitous Gram-negative bacterium, that is associated with significant disease in immunocompromised ..
Dietary zinc and the control of Streptococcus pneumoniae infection
Bart A Eijkelkamp, Jacqueline R Morey, Stephanie L Neville, Aimee Tan, Victoria G Pederick, Nerida Cole, Prashina P Singh, Cheryl-Lynn Y Ong, Raquel Gonzalez de Vega, David Clases, Bliss A Cunningham, Catherine E Hughes, Iain Comerford, Erin B Brazel, Jonathan J Whittall, Charles D Plumptre, Shaun R McColl, James C Paton, Alastair G McEwan, Philip A Doble
Zinc-binding to the cytoplasmic PAS domain regulates the essential WalK histidine kinase of Staphylococcus aureus
Ian R Monk, Nausad Shaikh, Stephanie L Begg, Mike Gajdiss, Liam KR Sharkey, Jean YH Lee, Sacha J Pidot, Torsten Seemann, Michael Kuiper, Brit Winnen, Rikki Hvorup, Brett M Collins, Gabriele Bierbaum, Saumya R Udagedara, Jacqueline R Morey, Neha Pulyani, Benjamin P Howden, Megan J Maher, Christopher A McDevitt, Glenn F King
Conformational and dynamic plasticity in substrate-binding proteins underlies selective transport in ABC importers
Marijn de Boer, Giorgos Gouridis, Ruslan Vietrov, Stephanie L Begg, Gea K Schuurman-Wolters, Florence Husada, Nikolaos Eleftheriadis, Bert Poolman, Christopher A McDevit, Thorben Cordes
The Role of the CopA Copper Efflux System in Acinetobacter baumannii Virulence
Saleh F Alquethamy, Marjan Khorvash, Victoria G Pederick, Jonathan J Whittall, James C Paton, Ian T Paulsen, Karl A Hassan, Christopher A McDevitt, Bart A Eijkelkamp
Acinetobacter baumannii has emerged as one of the leading causative agents of nosocomial infections. Due to its high level of intr..
Identification of Novel Acinetobacter baumannii Host Fatty Acid Stress Adaptation Strategies
Jhih-Hang Jiang, Karl A Hassan, Stephanie L Begg, Thusitha WT Rupasinghe, Varsha Naidu, Victoria G Pederick, Marjan Khorvash, Jonathan J Whittall, James C Paton, Ian T Paulsen, Christopher A McDevitt, Anton Y Peleg, Bart A Eijkelkamp
Free fatty acids hold important immune-modulatory roles during infection. However, the host's long-chain polyunsaturated fatty aci..
The Metallophore Staphylopine Enables Staphylococcus aureus To Compete with the Host for Zinc and Overcome Nutritional Immunity
Kyle P Grim, Brian San Francisco, Jana N Radin, Erin B Brazel, Jessica L Kelliher, Paola K Parraga Solorzano, Philip C Kim, Christopher A McDevitt, Thomas E Kehl-Fie
During infection, the host sequesters essential nutrients, such as zinc, to combat invading microbes. Despite the ability of the i..