UNDERSTANDING AND EXPLOITING HOW DIFFERENT CELL DEATH PATHWAYS CONTROL INTRACELLULAR INFECTIONS

Deletion of the transcriptional regulator TFAP4 accelerates c-MYC-driven lymphomagenesis.

Margaret A Potts, Shinsuke Mizutani, Alexandra L Garnham, Connie SN Li Wai Suen, Andrew J Kueh, Lin Tai, Martin Pal, Andreas Strasser, Marco J Herold

2023-03-09

Many lymphoid malignancies arise from deregulated c-MYC expression in cooperation with additional genetic lesions. While many of t..

An arrayed CRISPR screen of primary B cells reveals the essential elements of the antibody secretion pathway.

Stephanie Trezise, Isabella Y Kong, Edwin D Hawkins, Marco J Herold, Simon N Willis, Stephen L Nutt

2023-01-01

BACKGROUND: Humoral immunity depends on the differentiation of B cells into antibody secreting cells (ASCs). Excess or inappropria..

Generation of a CRISPR activation mouse that enables modelling of aggressive lymphoma and interrogation of venetoclax resistance

Yexuan Deng, Sarah T Diepstraten, Margaret A Potts, Goeknur Giner, Stephanie Trezise, Ashley P Ng, Gerry Healey, Serena R Kane, Amali Cooray, Kira Behrens, Amy Heidersbach, Andrew J Kueh, Martin Pal, Stephen Wilcox, Lin Tai, Warren S Alexander, Jane E Visvader, Stephen L Nutt, Andreas Strasser, Benjamin Haley

2022-08-12

CRISPR technologies have advanced cancer modelling in mice, but CRISPR activation (CRISPRa) methods have not been exploited in thi..

Last but not least: BFL-1 as an emerging target for anti-cancer therapies

Gaoyuan Wang, Sarah T Diepstraten, Marco J Herold

2022-07-28

BFL-1 is an understudied pro-survival BCL-2 protein. The expression of BFL-1 is reported in many cancers, but it is yet to be clar..

Epigenetic modulators of B cell fate identified through coupled phenotype-transcriptome analysis

Isabella Y Kong, Stephanie Trezise, Amanda Light, Izabela Todorovski, Gisela Mir Arnau, Sreeja Gadipally, David Yoannidis, Kaylene J Simpson, Xueyi Dong, Lachlan Whitehead, Jessica C Tempany, Anthony J Farchione, Amania A Sheikh, Joanna R Groom, Kelly L Rogers, Marco J Herold, Vanessa L Bryant, Matthew E Ritchie, Simon N Willis, Ricky W Johnstone

2022-07-13

High-throughput methodologies are the cornerstone of screening approaches to identify novel compounds that regulate immune cell fu..

Ubiquitin-like protein 3 (UBL3) is required for MARCH ubiquitination of major histocompatibility complex class II and CD86

Haiyin Liu, Kayla R Wilson, Ashley M Firth, Christophe Macri, Patrick Schriek, Annabelle B Blum, Javiera Villar, Samuel Wormald, Mitch Shambrook, Bangyan Xu, Hui Jing Lim, Hamish EG McWilliam, Andrew F Hill, Laura E Edgington-Mitchell, Irina Caminschi, Mireille H Lahoud, Elodie Segura, Marco J Herold, Jose A Villadangos, Justine D Mintern

2022-04-11

The MARCH E3 ubiquitin (Ub) ligase MARCH1 regulates trafficking of major histocompatibility complex class II (MHC II) and CD86, mo..

Removal of BFL-1 sensitises some melanoma cells to killing by BH3 mimetic drugs

Lahiru Gangoda, Robyn L Schenk, Lin Tai, Pacman Szeto, Jen G Cheung, Andreas Strasser, Guillaume Lessene, Mark Shackleton, Marco J Herold

2022-04-04

Loss of TRP53 reduces but does not overcome dependency of lymphoma cells on MCL-1

Brandon J Aubrey, Margs S Brennan, Sarah T Diepstraten, Zilu Wang, Catherine Chang, Marco J Herold, Andreas Strasser, Gemma L Kelly

2022-01-28

Caspase-2 does not play a critical role in cell death induction and bacterial clearance during Salmonella infection

Sven Engel, Marcel Doerflinger, Ariane R Lee, Andreas Strasser, Marco J Herold, Sammy Bedoui, Annabell Bachem

2021-10-20

Absence of pro-survival A1 has no impact on inflammatory cell survival in vivo during acute lung inflammation and peritonitis

Lahiru Gangoda, Robyn L Schenk, Sarah A Best, Christina Nedeva, Cynthia Louis, Damian B D'Silva, Kirsten Fairfax, Andrew G Jarnicki, Hamsa Puthalakath, Kate D Sutherland, Andreas Strasser, Marco J Herold

2021-07-24

Inflammation is a natural defence mechanism of the body to protect against pathogens. It is induced by immune cells, such as macro..

Ptpn2 and KLRG1 regulate the generation and in skin

Katharina Hochheiser, Florian Wiede, Teagan Wagner, David Freestone, Matthias H Enders, Moshe Olshansky, Brendan Russ, Simone Nussing, Emma Bawden, Asolina Braun, Annabell Bachem, Elise Gressier, Robyn McConville, Simone L Park, Claerwen M Jones, Gayle M Davey, David E Gyorki, David Tscharke, Ian A Parish, Stephen Turner

2021-06-07

Tissue-resident memory T cells (TRM cells) are key elements of tissue immunity. Here, we investigated the role of the regulator of..

Intracellular Staphylococcus aureus and host cell death pathways

Ye Mon Soe, Sammy Bedoui, Timothy P Stinear, Abderrahman Hachani

2021-02-24

Staphylococcus aureus is a major opportunistic human pathogen that is globally prevalent. Although S. aureus and humans may have c..

CRISPR base editing applications for identifying cancer-driving mutations

Martin Pal, Marco J Herold

2021-02-01

CRISPR base editing technology is a promising genome editing tool as (i) it does not require a DNA template to introduce mutations..

Critical cancer vulnerabilities identified by unbiased CRISPR/Cas9 screens inform on efficient cancer Immunotherapy

Margaret A Potts, Jackson A McDonald, Kate D Sutherland, Marco J Herold

2020-12-01

The mutational landscape of human cancers is highly complex. While next generation sequencing aims to comprehensively catalogue so..

Flexible Usage and Interconnectivity of Diverse Cell Death Pathways Protect against Intracellular Infection

Marcel Doerflinger, Yexuan Deng, Paul Whitney, Ranja Salvamoser, Sven Engel, Andrew J Kueh, Lin Tai, Annabell Bachem, Elise Gressier, Niall D Geoghegan, Stephen Wilcox, Kelly L Rogers, Alexandra L Garnham, Michael A Dengler, Stefanie M Bader, Gregor Ebert, Jaclyn S Pearson, Dominic De Nardo, Nancy Wang, Chenying Yang

2020-09-15

Programmed cell death contributes to host defense against pathogens. To investigate the relative importance of pyroptosis, necropt..

Emerging connectivity of programmed cell death pathways and its physiological implications

Sammy Bedoui, Marco J Herold, Andreas Strasser

2020-09-01

The removal of functionally dispensable, infected or potentially neoplastic cells is driven by programmed cell death (PCD) pathway..

Targeting platelets for improved outcome in KRAS-driven lung adenocarcinoma

Stephanie R Hyslop, Marliese Alexander, Alesha A Thai, Ariena Kersbergen, Andrew J Kueh, Marco J Herold, Jason Corbin, Pradnya Gangatirkar, Ashley P Ng, Benjamin J Solomon, Warren S Alexander, Kate D Sutherland, Emma C Josefsson

2020-07-16

Elevated platelet count is associated with poor survival in certain solid cancers, including lung cancer. In addition, experimenta..

The pro-survival Bcl-2 family member A1 delays spontaneous and FAS ligand-induced apoptosis of activated neutrophils

Robyn L Schenk, Lahiru Gangoda, Kate E Lawlor, Lorraine A O'Reilly, Andreas Strasser, Marco J Herold

2020-06-18

An Erg-driven transcriptional program controls B cell lymphopoiesis.

Ashley P Ng, Hannah D Coughlan, Soroor Hediyeh-Zadeh, Kira Behrens, Timothy M Johanson, Michael Sze Yuan Low, Charles C Bell, Omer Gilan, Yih-Chih Chan, Andrew J Kueh, Thomas Boudier, Rebecca Feltham, Anna Gabrielyan, Ladina DiRago, Craig D Hyland, Helen Ierino, Sandra Mifsud, Elizabeth Viney, Tracy Willson, Mark A Dawson

2020-06-15

B lymphoid development is initiated by the differentiation of hematopoietic stem cells into lineage committed progenitors, ultimat..