NEW MOLECULAR TOOLS TO STUDY THE MECHANISMS OF BACTERIAL METAL HOMEOSTASIS

The role of CopA in Streptococcus pyogenes copper homeostasis and virulence.

Tina H Dao, Amy Iverson, Stephanie L Neville, Michael DL Johnson, Christopher A McDevitt, Jason W Rosch

2023-03-01

Maintenance of intracellular metal homeostasis during interaction with host niches is critical to the success of bacterial pathoge..

Structural and biochemical characterization of Acinetobacter baumannii ZnuA

Saleh Alquethamy, Katherine Ganio, Zhenyao Luo, Sheikh I Hossain, Andrew J Hayes, Thomas Ve, Mark R Davies, Evelyne Deplazes, Bostjan Kobe, Christopher A McDevitt

2022-06-01

Acinetobacter baumannii is a Gram-negative nosocomial pathogen associated with significant disease. Crucial to the survival and pa..

The Impact of Chromate on Pseudomonas aeruginosa Molybdenum Homeostasis

Eve A Maunders, Dalton HY Ngu, Katherine Ganio, Sheikh I Hossain, Bryan YJ Lim, Michael G Leeming, Zhenyao Luo, Aimee Tan, Evelyne Deplazes, Bostjan Kobe, Christopher A McDevitt

2022-05-24

Acquisition of the trace-element molybdenum via the high-affinity ATP-binding cassette permease ModABC is essential for Pseudomona..

Neurodegenerative Disease Treatment Drug PBT2 Breaks Intrinsic Polymyxin Resistance in Gram-Positive Bacteria

David MP De Oliveira, Bernhard Keller, Andrew J Hayes, Cheryl-Lynn Y Ong, Nichaela Harbison-Price, Ibrahim M El-Deeb, Gen Li, Nadia Keller, Lisa Bohlmann, Stephan Brouwer, Andrew G Turner, Amanda J Cork, Thomas R Jones, David L Paterson, Alastair G McEwan, Mark R Davies, Christopher A McDevitt, Mark von Itzstein, Mark J Walker

2022-04-01

Gram-positive bacteria do not produce lipopolysaccharide as a cell wall component. As such, the polymyxin class of antibiotics, wh..

MntP and YiiP Contribute to Manganese Efflux in Salmonella enterica Serovar Typhimurium under Conditions of Manganese Overload and Nitrosative Stress

Annie Ouyang, Kendall M Gasner, Stephanie L Neville, Christopher A McDevitt, Elaine R Frawley

2022-02-01

The divalent transition metal cation manganese is important for protein function, particularly under conditions of iron limitation..

The Role of ZntA in Klebsiella pneumoniae Zinc Homeostasis

Eve A Maunders, Katherine Ganio, Andrew J Hayes, Stephanie L Neville, Mark R Davies, Richard A Strugnell, Christopher A McDevitt, Aimee Tan

2022-02-01

Klebsiella pneumoniae is an opportunistic Gram-negative pathogen that is a leading cause of healthcare-associated infections, incl..

Dysregulation of Streptococcus pneumoniae zinc homeostasis breaks ampicillin resistance in a pneumonia infection model

Erin B Brazel, Aimee Tan, Stephanie L Neville, Amy R Iverson, Saumya R Udagedara, Bliss A Cunningham, Mwilye Sikanyika, David MP De Oliveira, Bernhard Keller, Lisa Bohlmann, Ibrahim M El-Deeb, Katherine Ganio, Bart A Eijkelkamp, Alastair G McEwan, Mark von Itzstein, Megan J Maher, Mark J Walker, Jason W Rosch, Christopher A McDevitt

2022-01-11

Streptococcus pneumoniae is the primary cause of community-acquired bacterial pneumonia with rates of penicillin and multidrug-res..

The Molecular Basis of Acinetobacter baumannii Cadmium Toxicity and Resistance

Saleh F Alquethamy, Felise G Adams, Ram Maharjan, Natasha N Delgado, Maoge Zang, Katherine Ganio, James C Paton, Karl A Hassan, Ian T Paulsen, Christopher A McDevitt, Amy K Cain, Bart A Eijkelkamp

2021-11-01

Acinetobacter species are ubiquitous Gram-negative bacteria that can be found in water, in soil, and as commensals of the human sk..

Conformation of the Solute-Binding Protein AdcAII Influences Zinc Uptake in Streptococcus pneumoniae

Marina L Zupan, Zhenyao Luo, Katherine Ganio, Victoria G Pederick, Stephanie L Neville, Evelyne Deplazes, Bostjan Kobe, Christopher A McDevitt

2021-08-19

Streptococcus pneumoniae scavenges essential zinc ions from the host during colonization and infection. This is achieved by the AT..

The structural basis of bacterial manganese import

Stephanie L Neville, Jennie Sjoehamn, Jacinta A Watts, Hugo MacDermott-Opeskin, Stephen J Fairweather, Katherine Ganio, Alex Carey Hulyer, Aaron P McGrath, Andrew J Hayes, Tess R Malcolm, Mark R Davies, Norimichi Nomura, So Iwata, Megan L O'Mara, Megan J Maher, Christopher A McDevitt

2021-08-01

Metal ions are essential for all forms of life. In prokaryotes, ATP-binding cassette (ABC) permeases serve as the primary import p..

Absence of high priority critically important antimicrobial resistance in Salmonella sp. isolated from Australian commercial egg layer environments

Tania Veltman, David Jordan, Christopher A McDevitt, Jan Bell, Benjamin P Howden, Mary Valcanis, Mark O'Dea, Sam Abraham, Peter Scott, Jessica H Kovac, Raymond Chia, Barry Combs, Kapil Chousalkar, Timothy Wilson, Darren J Trott

2021-01-16

The development of antimicrobial resistance in foodborne pathogens is a growing public health concern. This study was undertaken t..

A Trap-Door Mechanism for Zinc Acquisition by Streptococcus pneumoniae AdcA

Zhenyao Luo, Jacqueline R Morey, Evelyne Deplazes, Alina Motygullina, Aimee Tan, Katherine Ganio, Stephanie L Neville, Nikolaos Eleftheriadis, Michael Isselstein, Victoria G Pederick, James C Paton, Thorben Cordes, Jeffrey R Harmer, Bostjan Kobe, Christopher A McDevitt

2021-01-01

Zinc is an essential element in all domains of life. Nonetheless, how prokaryotes achieve selective acquisition of zinc from the e..

Cadmium stress dictates central carbon flux and alters membrane composition in Streptococcus pneumoniae

Stephanie L Neville, Bart A Eijkelkamp, Amber Lothian, James C Paton, Blaine R Roberts, Jason W Rosch, Christopher A McDevitt

2020-12-19

Metal ion homeostasis is essential for all forms of life. However, the breadth of intracellular impacts that arise upon dysregulat..

Manganese import protects Salmonella enterica serovar Typhimurium against nitrosative stress

Shehla Yousuf, Joyce E Karlinsey, Stephanie L Neville, Christopher A McDevitt, Stephen J Libby, Ferric C Fang, Elaine R Frawley

2020-11-01

Nitric oxide (NO˙) is a radical molecule produced by mammalian phagocytic cells as part of the innate immune response to bacterial..

Structural and functional characterizations of the C-terminal domains of CzcD proteins

Saumya R Udagedara, Daniel M La Porta, Christian Spehar, Ghruta Purohit, Matthew JA Hein, Monique E Fatmous, G Patricia Casas Garcia, Katherine Ganio, Christopher A McDevitt, Megan J Maher

2020-07-01

Zinc is a potent antimicrobial component of the innate immune response at the host-pathogen interface. Bacteria subvert or resist ..

Disruption of Phosphate Homeostasis Sensitizes Staphylococcus aureus to Nutritional Immunity

Jessica L Kelliher, Erin B Brazel, Jana N Radin, Eliot S Joya, Paola K Parraga Solorzano, Stephanie L Neville, Christopher A McDevitt, Thomas E Kehl-Fie

2020-05-01

To control infection, mammals actively withhold essential nutrients, including the transition metal manganese, by a process termed..

Experimental Evolution In Vivo To Identify Selective Pressures during Pneumococcal Colonization

Vaughn S Cooper, Erin Honsa, Hannah Rowe, Christopher Deitrick, Amy R Iverson, Jonathan J Whittall, Stephanie L Neville, Christopher A McDevitt, Colin Kietzman, Jason W Rosch

2020-05-01

Experimental evolution is a powerful technique to understand how populations evolve from selective pressures imparted by the surro..

Intracellular Accumulation of Staphylopine Can Sensitize Staphylococcus aureus to Host-Imposed Zinc Starvation by Chelation-Independent Toxicity

Kyle P Grim, Jana N Radin, Paola K Parraga Solorzano, Jacqueline R Morey, Katie A Frye, Katherine Ganio, Stephanie L Neville, Christopher A McDevitt, Thomas E Kehl-Fie

2020-05-01

The host restricts the availability of zinc to prevent infection. To overcome this defense, Staphylococcus aureus and Pseudomonas ..

Comparing Nonbonded Metal Ion Models in the Divalent Cation Binding Protein PsaA

Hugo MacDermott-Opeskin, Christopher A McDevitt, Megan L O'Mara

2020-03-01

Divalent metal cations are essential for many biological processes; however, accurately modeling divalent metal ions has proved a ..

Structure and Metal Binding Properties of Chlamydia trachomatis YtgA

Zhenyao Luo, Stephanie L Neville, Rebecca Campbell, Jacqueline R Morey, Shruti Menon, Mark Thomas, Bart A Eijkelkamp, Miranda P Ween, Wilhelmina M Huston, Bostjan Kobe, Christopher A McDevitt

2020-01-01

The obligate intracellular pathogen Chlamydia trachomatis is a globally significant cause of sexually transmitted bacterial infect..

The Role of Zinc Efflux during Acinetobacter baumannii Infection

Saleh F Alquethamy, Felise G Adams, Varsha Naidu, Marian Khorvash, Victoria G Pederick, Maoge Zang, James C Paton, Ian T Paulsen, Karl A Hassan, Amy K Cain, Christopher A McDevitt, Bart A Eijkelkamp

2020-01-01

Acinetobacter baumannii is a ubiquitous Gram-negative bacterium, that is associated with significant disease in immunocompromised ..

Dietary zinc and the control of Streptococcus pneumoniae infection

Bart A Eijkelkamp, Jacqueline R Morey, Stephanie L Neville, Aimee Tan, Victoria G Pederick, Nerida Cole, Prashina P Singh, Cheryl-Lynn Y Ong, Raquel Gonzalez de Vega, David Clases, Bliss A Cunningham, Catherine E Hughes, Iain Comerford, Erin B Brazel, Jonathan J Whittall, Charles D Plumptre, Shaun R McColl, James C Paton, Alastair G McEwan, Philip A Doble

2019-08-01

Human zinc deficiency increases susceptibility to bacterial infection. Although zinc supplementation therapies can reduce the impa..

Zinc-binding to the cytoplasmic PAS domain regulates the essential WalK histidine kinase of Staphylococcus aureus

Ian R Monk, Nausad Shaikh, Stephanie L Begg, Mike Gajdiss, Liam KR Sharkey, Jean YH Lee, Sacha J Pidot, Torsten Seemann, Michael Kuiper, Brit Winnen, Rikki Hvorup, Brett M Collins, Gabriele Bierbaum, Saumya R Udagedara, Jacqueline R Morey, Neha Pulyani, Benjamin P Howden, Megan J Maher, Christopher A McDevitt, Glenn F King

2019-07-11

WalKR (YycFG) is the only essential two-component regulator in the human pathogen Staphylococcus aureus. WalKR regulates peptidogl..

Cr-Ag coatings: synthesis, microstructure and antimicrobial properties

Afshin Karami, Hu Zhang, Victoria G Pederick, Christopher A McDevitt, Mohammad Sharear Kabir, Song Xu, Paul Munroe, Zhifeng Zhou, Zonghan Xie

2019-07-03

Cr–Ag coatings for antimicrobial surface applications were fabricated by magnetron sputtering. The coating microstructure and mech..

Defining the Role of the Streptococcus agalactiae Sht-Family Proteins in Zinc Acquisition and Complement Evasion

P Moulin, V Rong, A Ribeiro E Silva, VG Pederick, E Camlade, L Mereghetti, CA McDevitt, A Hiron

2019-04-01

Streptococcus agalactiae is not only part of the human intestinal and urogenital microbiota but is also a leading cause of septice..

Conformational and dynamic plasticity in substrate-binding proteins underlies selective transport in ABC importers

Marijn de Boer, Giorgos Gouridis, Ruslan Vietrov, Stephanie L Begg, Gea K Schuurman-Wolters, Florence Husada, Nikolaos Eleftheriadis, Bert Poolman, Christopher A McDevit, Thorben Cordes

2019-03-22

Substrate-binding proteins (SBPs) are associated with ATP-binding cassette importers and switch from an open to a closed conformat..

The Role of the CopA Copper Efflux System in Acinetobacter baumannii Virulence

Saleh F Alquethamy, Marjan Khorvash, Victoria G Pederick, Jonathan J Whittall, James C Paton, Ian T Paulsen, Karl A Hassan, Christopher A McDevitt, Bart A Eijkelkamp

2019-02-01

Acinetobacter baumannii has emerged as one of the leading causative agents of nosocomial infections. Due to its high level of intr..

Identification of Novel Acinetobacter baumannii Host Fatty Acid Stress Adaptation Strategies

Jhih-Hang Jiang, Karl A Hassan, Stephanie L Begg, Thusitha WT Rupasinghe, Varsha Naidu, Victoria G Pederick, Marjan Khorvash, Jonathan J Whittall, James C Paton, Ian T Paulsen, Christopher A McDevitt, Anton Y Peleg, Bart A Eijkelkamp

2019-01-01

Free fatty acids hold important immune-modulatory roles during infection. However, the host's long-chain polyunsaturated fatty aci..

Synergy between Nutritional Immunity and Independent Host Defenses Contributes to the Importance of the MntABC Manganese Transporter during Staphylococcus aureus Infection

Jana N Radin, Jamie Zhu, Erin B Brazel, Christopher A McDevitt, Thomas E Kehl-Fie

2019-01-01

During infection, the host utilizes a diverse array of processes to combat invaders, including the restriction of availability of ..

A Liposomal Platform for Sensing of Extracellular Analytes Near Cells

Xiaozhou Zhang, Sabrina Heng, Jinxin Pei, Jacqueline R Morey, Christopher A McDevitt, Andrew D Abell

2018-12-01

Cell-permeable fluorescent chemosensors (calcein, monochlorobimane, and a recently reported spiropyran-based sensor SP2) have been..

Antimicrobial Susceptibility of Escherichia coli and Salmonella spp. Isolates From Healthy Pigs in Australia: Results of a Pilot National Survey

Amanda K Kidsley, Sam Abraham, Jan M Bell, Mark O'Dea, Tanya J Laird, David Jordan, Pat Mitchell, Christopher A McDevitt, Darren J Trott

2018-07-09

This study investigated the frequency of antimicrobial non-susceptibility (defined as the frequency of isolates with minimum inhib..

Structural characterisation of the HT3 motif of the polyhistidine triad protein D from Streptococcus pneumoniae

Zhenyao Luo, Victoria G Pederick, James C Paton, Christopher A McDevitt, Bostjan Kobe

2018-07-01

The bacterium Streptococcus pneumoniae (the pneumococcus) is a major human pathogen that requires Zn2+ for its survival and virule..

Arachidonic Acid Stress Impacts Pneumococcal Fatty Acid Homeostasis

Bart A Eijkelkamp, Stephanie L Begg, Victoria G Pederick, Claudia Trapetti, Melissa K Gregory, Jonathan J Whittall, James C Paton, Christopher A McDevitt

2018-05-11

Free fatty acids hold dual roles during infection, serving to modulate the host immune response while also functioning directly as..