NEW MOLECULAR TOOLS TO STUDY THE MECHANISMS OF BACTERIAL METAL HOMEOSTASIS

Conformation of the Solute-Binding Protein AdcAII Influences Zinc Uptake in Streptococcus pneumoniae

Marina L Zupan, Zhenyao Luo, Katherine Ganio, Victoria G Pederick, Stephanie L Neville, Evelyne Deplazes, Bostjan Kobe, Christopher A McDevitt

2021-08-19

Streptococcus pneumoniae scavenges essential zinc ions from the host during colonization and infection. This is achieved by the AT..

The structural basis of bacterial manganese import

Stephanie L Neville, Jennie Sjoehamn, Jacinta A Watts, Hugo MacDermott-Opeskin, Stephen J Fairweather, Katherine Ganio, Alex Carey Hulyer, Aaron P McGrath, Andrew J Hayes, Tess R Malcolm, Mark R Davies, Norimichi Nomura, So Iwata, Megan L O'Mara, Megan J Maher, Christopher A McDevitt

2021-08-01

Metal ions are essential for all forms of life. In prokaryotes, ATP-binding cassette (ABC) permeases serve as the primary import p..

Cadmium stress dictates central carbon flux and alters membrane composition in Streptococcus pneumoniae

Stephanie L Neville, Bart A Eijkelkamp, Amber Lothian, James C Paton, Blaine R Roberts, Jason W Rosch, Christopher A McDevitt

2020-12-19

Metal ion homeostasis is essential for all forms of life. However, the breadth of intracellular impacts that arise upon dysregulat..

Manganese import protects Salmonella enterica serovar Typhimurium against nitrosative stress

Shehla Yousuf, Joyce E Karlinsey, Stephanie L Neville, Christopher A McDevitt, Stephen J Libby, Ferric C Fang, Elaine R Frawley

2020-11-01

Nitric oxide (NO˙) is a radical molecule produced by mammalian phagocytic cells as part of the innate immune response to bacterial..

Structural and functional characterizations of the C-terminal domains of CzcD proteins

Saumya R Udagedara, Daniel M La Porta, Christian Spehar, Ghruta Purohit, Matthew JA Hein, Monique E Fatmous, G Patricia Casas Garcia, Katherine Ganio, Christopher A McDevitt, Megan J Maher

2020-07-01

Zinc is a potent antimicrobial component of the innate immune response at the host-pathogen interface. Bacteria subvert or resist ..

Disruption of Phosphate Homeostasis Sensitizes Staphylococcus aureus to Nutritional Immunity

Jessica L Kelliher, Erin B Brazel, Jana N Radin, Eliot S Joya, Paola K Parraga Solorzano, Stephanie L Neville, Christopher A McDevitt, Thomas E Kehl-Fie

2020-05-01

To control infection, mammals actively withhold essential nutrients, including the transition metal manganese, by a process termed..

Experimental Evolution In Vivo To Identify Selective Pressures during Pneumococcal Colonization

Vaughn S Cooper, Erin Honsa, Hannah Rowe, Christopher Deitrick, Amy R Iverson, Jonathan J Whittall, Stephanie L Neville, Christopher A McDevitt, Colin Kietzman, Jason W Rosch

2020-05-01

Experimental evolution is a powerful technique to understand how populations evolve from selective pressures imparted by the surro..

Intracellular Accumulation of Staphylopine Can Sensitize Staphylococcus aureus to Host-Imposed Zinc Starvation by Chelation-Independent Toxicity

Kyle P Grim, Jana N Radin, Paola K Parraga Solorzano, Jacqueline R Morey, Katie A Frye, Katherine Ganio, Stephanie L Neville, Christopher A McDevitt, Thomas E Kehl-Fie

2020-05-01

The host restricts the availability of zinc to prevent infection. To overcome this defense, Staphylococcus aureus and Pseudomonas ..

Comparing Nonbonded Metal Ion Models in the Divalent Cation Binding Protein PsaA

Hugo MacDermott-Opeskin, Christopher A McDevitt, Megan L O'Mara

2020-03-01

Divalent metal cations are essential for many biological processes; however, accurately modeling divalent metal ions has proved a ..

Experimental Evolution in vivo to Identify Selective Pressures During Pneumococcal Colonization

Vaughn Cooper, Erin Honsa, Hannah Rowe, Christopher Deitrick, Amy Iverson, Jonathan Whittall, Stephanie Neville, Christopher McDevitt, Colin Kietzman, Jason Rosch

2020-01-16

Experimental evolution is a powerful technique to understand how populations evolve from selective pressures imparted by the surro..

Structure and Metal Binding Properties of Chlamydia trachomatis YtgA

Zhenyao Luo, Stephanie L Neville, Rebecca Campbell, Jacqueline R Morey, Shruti Menon, Mark Thomas, Bart A Eijkelkamp, Miranda P Ween, Wilhelmina M Huston, Bostjan Kobe, Christopher A McDevitt

2020-01-01

The obligate intracellular pathogen Chlamydia trachomatis is a globally significant cause of sexually transmitted bacterial infect..

The Role of Zinc Efflux during Acinetobacter baumannii Infection

Saleh F Alquethamy, Felise G Adams, Varsha Naidu, Marian Khorvash, Victoria G Pederick, Maoge Zang, James C Paton, Ian T Paulsen, Karl A Hassan, Amy K Cain, Christopher A McDevitt, Bart A Eijkelkamp

2020-01-01

Acinetobacter baumannii is a ubiquitous Gram-negative bacterium, that is associated with significant disease in immunocompromised ..

Dietary zinc and the control of Streptococcus pneumoniae infection

Bart A Eijkelkamp, Jacqueline R Morey, Stephanie L Neville, Aimee Tan, Victoria G Pederick, Nerida Cole, Prashina P Singh, Cheryl-Lynn Y Ong, Raquel Gonzalez de Vega, David Clases, Bliss A Cunningham, Catherine E Hughes, Iain Comerford, Erin B Brazel, Jonathan J Whittall, Charles D Plumptre, Shaun R McColl, James C Paton, Alastair G McEwan, Philip A Doble

2019-08-01

Human zinc deficiency increases susceptibility to bacterial infection. Although zinc supplementation therapies can reduce the impa..

Zinc-binding to the cytoplasmic PAS domain regulates the essential WalK histidine kinase of Staphylococcus aureus

Ian R Monk, Nausad Shaikh, Stephanie L Begg, Mike Gajdiss, Liam KR Sharkey, Jean YH Lee, Sacha J Pidot, Torsten Seemann, Michael Kuiper, Brit Winnen, Rikki Hvorup, Brett M Collins, Gabriele Bierbaum, Saumya R Udagedara, Jacqueline R Morey, Neha Pulyani, Benjamin P Howden, Megan J Maher, Christopher A McDevitt, Glenn F King

2019-07-11

WalKR (YycFG) is the only essential two-component regulator in the human pathogen Staphylococcus aureus. WalKR regulates peptidogl..

Defining the Role of the Streptococcus agalactiae Sht-Family Proteins in Zinc Acquisition and Complement Evasion

P Moulin, V Rong, A Ribeiro E Silva, VG Pederick, E Camlade, L Mereghetti, CA McDevitt, A Hiron

2019-04-01

Streptococcus agalactiae is not only part of the human intestinal and urogenital microbiota but is also a leading cause of septice..

Conformational and dynamic plasticity in substrate-binding proteins underlies selective transport in ABC importers

Marijn de Boer, Giorgos Gouridis, Ruslan Vietrov, Stephanie L Begg, Gea K Schuurman-Wolters, Florence Husada, Nikolaos Eleftheriadis, Bert Poolman, Christopher A McDevit, Thorben Cordes

2019-03-22

Substrate-binding proteins (SBPs) are associated with ATP-binding cassette importers and switch from an open to a closed conformat..

The Role of the CopA Copper Efflux System in Acinetobacter baumannii Virulence

Saleh F Alquethamy, Marjan Khorvash, Victoria G Pederick, Jonathan J Whittall, James C Paton, Ian T Paulsen, Karl A Hassan, Christopher A McDevitt, Bart A Eijkelkamp

2019-02-01

Acinetobacter baumannii has emerged as one of the leading causative agents of nosocomial infections. Due to its high level of intr..

Identification of Novel Acinetobacter baumannii Host Fatty Acid Stress Adaptation Strategies

Jhih-Hang Jiang, Karl A Hassan, Stephanie L Begg, Thusitha WT Rupasinghe, Varsha Naidu, Victoria G Pederick, Marjan Khorvash, Jonathan J Whittall, James C Paton, Ian T Paulsen, Christopher A McDevitt, Anton Y Peleg, Bart A Eijkelkamp

2019-01-01

Free fatty acids hold important immune-modulatory roles during infection. However, the host's long-chain polyunsaturated fatty aci..

Synergy between Nutritional Immunity and Independent Host Defenses Contributes to the Importance of the MntABC Manganese Transporter during Staphylococcus aureus Infection

Jana N Radin, Jamie Zhu, Erin B Brazel, Christopher A McDevitt, Thomas E Kehl-Fie

2019-01-01

During infection, the host utilizes a diverse array of processes to combat invaders, including the restriction of availability of ..

A Liposomal Platform for Sensing of Extracellular Analytes Near Cells

Xiaozhou Zhang, Sabrina Heng, Jinxin Pei, Jacqueline R Morey, Christopher A McDevitt, Andrew D Abell

2018-12-01

Cell-permeable fluorescent chemosensors (calcein, monochlorobimane, and a recently reported spiropyran-based sensor SP2) have been..

Structural characterisation of the HT3 motif of the polyhistidine triad protein D from Streptococcus pneumoniae

Zhenyao Luo, Victoria G Pederick, James C Paton, Christopher A McDevitt, Bostjan Kobe

2018-07-01

The bacterium Streptococcus pneumoniae (the pneumococcus) is a major human pathogen that requires Zn2+ for its survival and virule..

Arachidonic Acid Stress Impacts Pneumococcal Fatty Acid Homeostasis

Bart A Eijkelkamp, Stephanie L Begg, Victoria G Pederick, Claudia Trapetti, Melissa K Gregory, Jonathan J Whittall, James C Paton, Christopher A McDevitt

2018-05-11

Free fatty acids hold dual roles during infection, serving to modulate the host immune response while also functioning directly as..