NEW MOLECULAR TOOLS TO STUDY THE MECHANISMS OF BACTERIAL METAL HOMEOSTASIS
Grant number: FT170100006 | Funding period: 2018 - 2021
This project aims to provide new insight into how metal ion uptake is regulated. It will precisely measure the cellular concentrations of metal ions, reveal the roles of metal ions in essential cellular processes, and identify the molecular targets of metal toxicity. Metal ions are essential to all forms of life and are used by up to half of all proteins to facilitate cellular chemical processes. The intended outcome of the research is to provide new fundamental knowledge of the roles of metal ions in bacterial cells; knowledge that will be key to defining the chemical biology of living systems and will provide information essential to understanding how microbes adapt to changing environment..View full description
Related publications (33)
The role of CopA in Streptococcus pyogenes copper homeostasis and virulence.
Tina H Dao, Amy Iverson, Stephanie L Neville, Michael DL Johnson, Christopher A McDevitt, Jason W Rosch
Maintenance of intracellular metal homeostasis during interaction with host niches is critical to the success of bacterial pathoge..
Structural and biochemical characterization of Acinetobacter baumannii ZnuA
Saleh Alquethamy, Katherine Ganio, Zhenyao Luo, Sheikh I Hossain, Andrew J Hayes, Thomas Ve, Mark R Davies, Evelyne Deplazes, Bostjan Kobe, Christopher A McDevitt
Acinetobacter baumannii is a Gram-negative nosocomial pathogen associated with significant disease. Crucial to the survival and pa..
The Impact of Chromate on Pseudomonas aeruginosa Molybdenum Homeostasis
Eve A Maunders, Dalton HY Ngu, Katherine Ganio, Sheikh I Hossain, Bryan YJ Lim, Michael G Leeming, Zhenyao Luo, Aimee Tan, Evelyne Deplazes, Bostjan Kobe, Christopher A McDevitt
Acquisition of the trace-element molybdenum via the high-affinity ATP-binding cassette permease ModABC is essential for Pseudomona..
Neurodegenerative Disease Treatment Drug PBT2 Breaks Intrinsic Polymyxin Resistance in Gram-Positive Bacteria
David MP De Oliveira, Bernhard Keller, Andrew J Hayes, Cheryl-Lynn Y Ong, Nichaela Harbison-Price, Ibrahim M El-Deeb, Gen Li, Nadia Keller, Lisa Bohlmann, Stephan Brouwer, Andrew G Turner, Amanda J Cork, Thomas R Jones, David L Paterson, Alastair G McEwan, Mark R Davies, Christopher A McDevitt, Mark von Itzstein, Mark J Walker
Gram-positive bacteria do not produce lipopolysaccharide as a cell wall component. As such, the polymyxin class of antibiotics, wh..
MntP and YiiP Contribute to Manganese Efflux in Salmonella enterica Serovar Typhimurium under Conditions of Manganese Overload and Nitrosative Stress
Annie Ouyang, Kendall M Gasner, Stephanie L Neville, Christopher A McDevitt, Elaine R Frawley
The divalent transition metal cation manganese is important for protein function, particularly under conditions of iron limitation..
The Role of ZntA in Klebsiella pneumoniae Zinc Homeostasis
Eve A Maunders, Katherine Ganio, Andrew J Hayes, Stephanie L Neville, Mark R Davies, Richard A Strugnell, Christopher A McDevitt, Aimee Tan
Klebsiella pneumoniae is an opportunistic Gram-negative pathogen that is a leading cause of healthcare-associated infections, incl..
Dysregulation of Streptococcus pneumoniae zinc homeostasis breaks ampicillin resistance in a pneumonia infection model
Erin B Brazel, Aimee Tan, Stephanie L Neville, Amy R Iverson, Saumya R Udagedara, Bliss A Cunningham, Mwilye Sikanyika, David MP De Oliveira, Bernhard Keller, Lisa Bohlmann, Ibrahim M El-Deeb, Katherine Ganio, Bart A Eijkelkamp, Alastair G McEwan, Mark von Itzstein, Megan J Maher, Mark J Walker, Jason W Rosch, Christopher A McDevitt
Streptococcus pneumoniae is the primary cause of community-acquired bacterial pneumonia with rates of penicillin and multidrug-res..
The Molecular Basis of Acinetobacter baumannii Cadmium Toxicity and Resistance
Saleh F Alquethamy, Felise G Adams, Ram Maharjan, Natasha N Delgado, Maoge Zang, Katherine Ganio, James C Paton, Karl A Hassan, Ian T Paulsen, Christopher A McDevitt, Amy K Cain, Bart A Eijkelkamp
Acinetobacter species are ubiquitous Gram-negative bacteria that can be found in water, in soil, and as commensals of the human sk..
Conformation of the Solute-Binding Protein AdcAII Influences Zinc Uptake in Streptococcus pneumoniae
Marina L Zupan, Zhenyao Luo, Katherine Ganio, Victoria G Pederick, Stephanie L Neville, Evelyne Deplazes, Bostjan Kobe, Christopher A McDevitt
Streptococcus pneumoniae scavenges essential zinc ions from the host during colonization and infection. This is achieved by the AT..
The structural basis of bacterial manganese import
Stephanie L Neville, Jennie Sjoehamn, Jacinta A Watts, Hugo MacDermott-Opeskin, Stephen J Fairweather, Katherine Ganio, Alex Carey Hulyer, Aaron P McGrath, Andrew J Hayes, Tess R Malcolm, Mark R Davies, Norimichi Nomura, So Iwata, Megan L O'Mara, Megan J Maher, Christopher A McDevitt
Metal ions are essential for all forms of life. In prokaryotes, ATP-binding cassette (ABC) permeases serve as the primary import p..
Absence of high priority critically important antimicrobial resistance in Salmonella sp. isolated from Australian commercial egg layer environments
Tania Veltman, David Jordan, Christopher A McDevitt, Jan Bell, Benjamin P Howden, Mary Valcanis, Mark O'Dea, Sam Abraham, Peter Scott, Jessica H Kovac, Raymond Chia, Barry Combs, Kapil Chousalkar, Timothy Wilson, Darren J Trott
The development of antimicrobial resistance in foodborne pathogens is a growing public health concern. This study was undertaken t..
A Trap-Door Mechanism for Zinc Acquisition by Streptococcus pneumoniae AdcA
Zhenyao Luo, Jacqueline R Morey, Evelyne Deplazes, Alina Motygullina, Aimee Tan, Katherine Ganio, Stephanie L Neville, Nikolaos Eleftheriadis, Michael Isselstein, Victoria G Pederick, James C Paton, Thorben Cordes, Jeffrey R Harmer, Bostjan Kobe, Christopher A McDevitt
Zinc is an essential element in all domains of life. Nonetheless, how prokaryotes achieve selective acquisition of zinc from the e..
Cadmium stress dictates central carbon flux and alters membrane composition in Streptococcus pneumoniae
Stephanie L Neville, Bart A Eijkelkamp, Amber Lothian, James C Paton, Blaine R Roberts, Jason W Rosch, Christopher A McDevitt
Metal ion homeostasis is essential for all forms of life. However, the breadth of intracellular impacts that arise upon dysregulat..
Manganese import protects Salmonella enterica serovar Typhimurium against nitrosative stress
Shehla Yousuf, Joyce E Karlinsey, Stephanie L Neville, Christopher A McDevitt, Stephen J Libby, Ferric C Fang, Elaine R Frawley
Nitric oxide (NO˙) is a radical molecule produced by mammalian phagocytic cells as part of the innate immune response to bacterial..
Structural and functional characterizations of the C-terminal domains of CzcD proteins
Saumya R Udagedara, Daniel M La Porta, Christian Spehar, Ghruta Purohit, Matthew JA Hein, Monique E Fatmous, G Patricia Casas Garcia, Katherine Ganio, Christopher A McDevitt, Megan J Maher
Zinc is a potent antimicrobial component of the innate immune response at the host-pathogen interface. Bacteria subvert or resist ..
Disruption of Phosphate Homeostasis Sensitizes Staphylococcus aureus to Nutritional Immunity
Jessica L Kelliher, Erin B Brazel, Jana N Radin, Eliot S Joya, Paola K Parraga Solorzano, Stephanie L Neville, Christopher A McDevitt, Thomas E Kehl-Fie
To control infection, mammals actively withhold essential nutrients, including the transition metal manganese, by a process termed..
Experimental Evolution In Vivo To Identify Selective Pressures during Pneumococcal Colonization
Vaughn S Cooper, Erin Honsa, Hannah Rowe, Christopher Deitrick, Amy R Iverson, Jonathan J Whittall, Stephanie L Neville, Christopher A McDevitt, Colin Kietzman, Jason W Rosch
Experimental evolution is a powerful technique to understand how populations evolve from selective pressures imparted by the surro..
Intracellular Accumulation of Staphylopine Can Sensitize Staphylococcus aureus to Host-Imposed Zinc Starvation by Chelation-Independent Toxicity
Kyle P Grim, Jana N Radin, Paola K Parraga Solorzano, Jacqueline R Morey, Katie A Frye, Katherine Ganio, Stephanie L Neville, Christopher A McDevitt, Thomas E Kehl-Fie
The host restricts the availability of zinc to prevent infection. To overcome this defense, Staphylococcus aureus and Pseudomonas ..
Structure and Metal Binding Properties of Chlamydia trachomatis YtgA
Zhenyao Luo, Stephanie L Neville, Rebecca Campbell, Jacqueline R Morey, Shruti Menon, Mark Thomas, Bart A Eijkelkamp, Miranda P Ween, Wilhelmina M Huston, Bostjan Kobe, Christopher A McDevitt
The obligate intracellular pathogen Chlamydia trachomatis is a globally significant cause of sexually transmitted bacterial infect..
The Role of Zinc Efflux during Acinetobacter baumannii Infection
Saleh F Alquethamy, Felise G Adams, Varsha Naidu, Marian Khorvash, Victoria G Pederick, Maoge Zang, James C Paton, Ian T Paulsen, Karl A Hassan, Amy K Cain, Christopher A McDevitt, Bart A Eijkelkamp
Acinetobacter baumannii is a ubiquitous Gram-negative bacterium, that is associated with significant disease in immunocompromised ..
Dietary zinc and the control of Streptococcus pneumoniae infection
Bart A Eijkelkamp, Jacqueline R Morey, Stephanie L Neville, Aimee Tan, Victoria G Pederick, Nerida Cole, Prashina P Singh, Cheryl-Lynn Y Ong, Raquel Gonzalez de Vega, David Clases, Bliss A Cunningham, Catherine E Hughes, Iain Comerford, Erin B Brazel, Jonathan J Whittall, Charles D Plumptre, Shaun R McColl, James C Paton, Alastair G McEwan, Philip A Doble
Human zinc deficiency increases susceptibility to bacterial infection. Although zinc supplementation therapies can reduce the impa..
Zinc-binding to the cytoplasmic PAS domain regulates the essential WalK histidine kinase of Staphylococcus aureus
Ian R Monk, Nausad Shaikh, Stephanie L Begg, Mike Gajdiss, Liam KR Sharkey, Jean YH Lee, Sacha J Pidot, Torsten Seemann, Michael Kuiper, Brit Winnen, Rikki Hvorup, Brett M Collins, Gabriele Bierbaum, Saumya R Udagedara, Jacqueline R Morey, Neha Pulyani, Benjamin P Howden, Megan J Maher, Christopher A McDevitt, Glenn F King
WalKR (YycFG) is the only essential two-component regulator in the human pathogen Staphylococcus aureus. WalKR regulates peptidogl..
Cr-Ag coatings: synthesis, microstructure and antimicrobial properties
Afshin Karami, Hu Zhang, Victoria G Pederick, Christopher A McDevitt, Mohammad Sharear Kabir, Song Xu, Paul Munroe, Zhifeng Zhou, Zonghan Xie
Cr–Ag coatings for antimicrobial surface applications were fabricated by magnetron sputtering. The coating microstructure and mech..
Defining the Role of the Streptococcus agalactiae Sht-Family Proteins in Zinc Acquisition and Complement Evasion
P Moulin, V Rong, A Ribeiro E Silva, VG Pederick, E Camlade, L Mereghetti, CA McDevitt, A Hiron
Streptococcus agalactiae is not only part of the human intestinal and urogenital microbiota but is also a leading cause of septice..
Conformational and dynamic plasticity in substrate-binding proteins underlies selective transport in ABC importers
Marijn de Boer, Giorgos Gouridis, Ruslan Vietrov, Stephanie L Begg, Gea K Schuurman-Wolters, Florence Husada, Nikolaos Eleftheriadis, Bert Poolman, Christopher A McDevit, Thorben Cordes
Substrate-binding proteins (SBPs) are associated with ATP-binding cassette importers and switch from an open to a closed conformat..
The Role of the CopA Copper Efflux System in Acinetobacter baumannii Virulence
Saleh F Alquethamy, Marjan Khorvash, Victoria G Pederick, Jonathan J Whittall, James C Paton, Ian T Paulsen, Karl A Hassan, Christopher A McDevitt, Bart A Eijkelkamp
Acinetobacter baumannii has emerged as one of the leading causative agents of nosocomial infections. Due to its high level of intr..
Identification of Novel Acinetobacter baumannii Host Fatty Acid Stress Adaptation Strategies
Jhih-Hang Jiang, Karl A Hassan, Stephanie L Begg, Thusitha WT Rupasinghe, Varsha Naidu, Victoria G Pederick, Marjan Khorvash, Jonathan J Whittall, James C Paton, Ian T Paulsen, Christopher A McDevitt, Anton Y Peleg, Bart A Eijkelkamp
Free fatty acids hold important immune-modulatory roles during infection. However, the host's long-chain polyunsaturated fatty aci..
Synergy between Nutritional Immunity and Independent Host Defenses Contributes to the Importance of the MntABC Manganese Transporter during Staphylococcus aureus Infection
Jana N Radin, Jamie Zhu, Erin B Brazel, Christopher A McDevitt, Thomas E Kehl-Fie
During infection, the host utilizes a diverse array of processes to combat invaders, including the restriction of availability of ..
A Liposomal Platform for Sensing of Extracellular Analytes Near Cells
Xiaozhou Zhang, Sabrina Heng, Jinxin Pei, Jacqueline R Morey, Christopher A McDevitt, Andrew D Abell
Cell-permeable fluorescent chemosensors (calcein, monochlorobimane, and a recently reported spiropyran-based sensor SP2) have been..
Antimicrobial Susceptibility of Escherichia coli and Salmonella spp. Isolates From Healthy Pigs in Australia: Results of a Pilot National Survey
Amanda K Kidsley, Sam Abraham, Jan M Bell, Mark O'Dea, Tanya J Laird, David Jordan, Pat Mitchell, Christopher A McDevitt, Darren J Trott
This study investigated the frequency of antimicrobial non-susceptibility (defined as the frequency of isolates with minimum inhib..
Structural characterisation of the HT3 motif of the polyhistidine triad protein D from Streptococcus pneumoniae
Zhenyao Luo, Victoria G Pederick, James C Paton, Christopher A McDevitt, Bostjan Kobe
The bacterium Streptococcus pneumoniae (the pneumococcus) is a major human pathogen that requires Zn2+ for its survival and virule..
Arachidonic Acid Stress Impacts Pneumococcal Fatty Acid Homeostasis
Bart A Eijkelkamp, Stephanie L Begg, Victoria G Pederick, Claudia Trapetti, Melissa K Gregory, Jonathan J Whittall, James C Paton, Christopher A McDevitt
Free fatty acids hold dual roles during infection, serving to modulate the host immune response while also functioning directly as..