Cutting the Fat: Photodissociation Mass Spectrometry for Lipidome Analysis
Grant number: DP190102464 | Funding period: 2019 - 2022
This project aims to develop and apply novel bioanalytical mass spectrometry-based methods and workflows to illuminate the otherwise hidden structural diversity and molecular complexity of the lipidome. The structure of individual lipids define their specific biological functions. A major requirement of analytical methods employed for lipid analysis on a lipidome-wide scale, therefore, is to enable the detailed structural characterisation of the, potentially, tens of thousands of individual molecular lipid species that may be present within a sample of interest. This project will develop and optimise novel, ultraviolet photodissociation-tandem mass spectrometry methods which will be integrat..View full description
Related publications (7)
Hybrid 213 nm photodissociation of cationized Sterol lipid ions yield [M]( center dot) Radical products for improved structural characterization using multistage tandem mass spectrometry
Henry West, Gavin E Reid
Sterols are a class of lipid molecules that include cholesterol, oxysterols, and sterol esters. Sterol lipids play critical functi..
Cardiolipin is required for membrane docking of mitochondrial ribosomes and protein synthesis
Richard G Lee, Junjie Gao, Stefan J Siira, Anne-Marie Shearwood, Judith A Ermer, Vinzenz Hofferek, James C Mathews, Minghao Zheng, Gavin E Reid, Oliver Rackham, Aleksandra Filipovska
The mitochondrial inner membrane contains a unique phospholipid known as cardiolipin (CL), which stabilises the protein complexes ..
A Novel Function of Sphingosine Kinase 2 in the Metabolism of Sphinga-4,14-Diene Lipids
Timothy Andrew Couttas, Yepy Hardi Rustam, Huitong Song, Yanfei Qi, Jonathan David Teo, Jinbiao Chen, Gavin Edmund Reid, Anthony Simon Don
The number, position, and configuration of double bonds in lipids affect membrane fluidity and the recruitment of signaling protei..
Evaluation of ultraviolet photodissociation tandem mass spectrometry for the structural assignment of unsaturated fatty acid double bond positional isomers
Mengxuan Fang, Yepy Rustam, Michelle Palmieri, Oliver M Sieber, Gavin E Reid
Fatty acids are a major source of structural diversity within the lipidome due to variations in their acyl chain lengths, branchin..
Establishing Signature Fragments for Identification and Sequencing of Dityrosine Cross-Linked Peptides Using Ultraviolet Photodissociation Mass Spectrometry
Soumya Mukherjee, Mengxuan Fang, W Mei Kok, Eugene A Kapp, Varsha J Thombare, Romain Huguet, Craig A Hutton, Gavin E Reid, Blaine R Roberts
Dityrosine cross-linking of Aβ peptides and α-synuclein is increasingly becoming recognized as a biomarker of neuropathological di..