Elucidating the Expression and Splicing of Neuropsychiatric Disease Genes in Human Brain With Nanopore Sequencing.

Isoform-level profiling of m6A epitranscriptomic signatures in human brain.

Josie Gleeson, Sachithrani U Madugalle, Ching Yin Wan, Catriona McLean, Timothy W Bredy, Ricardo De Paoli-Iseppi, Michael B Clark

2025-08-08

The RNA modification N6-methyladenosine (m6A) is highly abundant in human brain and implicated in neurological disorders. Profilin..

Long-read sequencing reveals the RNA isoform repertoire of neuropsychiatric risk genes in human brain

R De Paoli-Iseppi, SS Joshi, J Gleeson, YDJ Prawer, Y You, R Agarwal, A Li, A Hull, EM Whitehead, Y Seo, R Kujawa, R Chang, M Dutt, C McLean, BL Parker, MB Clark

2025-01-01

Background: Neuropsychiatric disorders are highly complex conditions and the risk of developing a disorder has been tied to hundre..

Pervasive effects of RNA degradation on Nanopore direct RNA sequencing

Yair DJ Prawer, Josie Gleeson, Ricardo De Paoli-Iseppi, Michael B Clark

2023-03-29

Oxford Nanopore direct RNA sequencing (DRS) is capable of sequencing complete RNA molecules and accurately measuring gene and isof..

Using long-read RNA sequencing to decipher the role of RNA isoforms in disease

Ricardo De Paoli-Iseppi, Shweta Joshi, Tomasz Wrzesinski, Paul J Harrison, Wilfried Haerty, Elizabeth M Tunbridge, Michael B Clark

2022-03-01

Accurate quantification of genes and their mRNA products is essential to understanding health and disease. In humans, processes su..