THE STRUCTURAL BIOLOGY OF TRACE METAL TRAFFICKING ACROSS MEMBRANES

Grant number: FT180100397 | Funding period: 2019 - 2023

Completed

Abstract

This project aims to investigate how essential trace element nutrients are recognised and specifically acquired and expelled by bacterial cells. Cells are surrounded by biomembranes that separate and protect them from their environments. Embedded within these membranes are proteins that perform essential functions. In bacteria, membrane proteins are responsible for the uptake and elimination of trace elements that are required for survival. This project will investigate the features of integral membrane proteins that allow discrimination between cargo, by defining their three dimensional architectures using X-ray crystallography. This will contribute to the field of membrane protein structur..

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Related publications (6)

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Mitochondrial COA7 is a heme-binding protein with disulfide reductase activity, which acts in the early stages of complex IV assembly

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Cytochrome c oxidase (COX) assembly factor 7 (COA7) is a metazoan-specific assembly factor, critical for the biogenesis of mitocho..

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Dysregulation of Streptococcus pneumoniae zinc homeostasis breaks ampicillin resistance in a pneumonia infection model

Erin B Brazel, Aimee Tan, Stephanie L Neville, Amy R Iverson, Saumya R Udagedara, Bliss A Cunningham, Mwilye Sikanyika, David MP De Oliveira, Bernhard Keller, Lisa Bohlmann, Ibrahim M El-Deeb, Katherine Ganio, Bart A Eijkelkamp, Alastair G McEwan, Mark von Itzstein, Megan J Maher, Mark J Walker, Jason W Rosch, Christopher A McDevitt

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Streptococcus pneumoniae is the primary cause of community-acquired bacterial pneumonia with rates of penicillin and multidrug-res..

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The structural basis of bacterial manganese import

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2021-08-01

Metal ions are essential for all forms of life. In prokaryotes, ATP-binding cassette (ABC) permeases serve as the primary import p..

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Structural and Functional Investigation of the Periplasmic Arsenate-Binding Protein ArrX from Chrysiogenes arsenatis

Nilakhi Poddar, Consuelo Badilla, Shadi Maghool, Thomas H Osborne, Joanne M Santini, Megan J Maher

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The anaerobic bacterium Chrysiogenes arsenatis respires using the oxyanion arsenate (AsO43-) as the terminal electron acceptor, wh..

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What Role Does COA6 Play in Cytochrome C Oxidase Biogenesis: A Metallochaperone or Thiol Oxidoreductase, or Both?

Shadi Maghool, Michael T Ryan, Megan J Maher

2020-10-01

Complex IV (cytochrome c oxidase; COX) is the terminal complex of the mitochondrial electron transport chain. Copper is essential ..

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Structural and functional characterizations of the C-terminal domains of CzcD proteins

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2020-07-01

Zinc is a potent antimicrobial component of the innate immune response at the host-pathogen interface. Bacteria subvert or resist ..

University of Melbourne Researchers