Live Cell Super Resolution Imaging Facility

Developing a Gram-Negative Selective Peptide–Drug Conjugate

TNG Handley, A Brakel, A Maxwell, J Ding, S Hadjigol, K D’Costa, C Chandrashekar, M Alder, MA Sani, GA Mackay, N O’Brien-Simpson, R Hoffmann, JD Wade, MA Hossain

2025-08-12

Resistance to fluoroquinolone antibiotics has serious implications for healthcare; here, we conjugate the widely used fluoroquinol..

Lipidated SNAPP-Stars Target and Kill Multidrug-Resistant Bacteria within Minutes

S Hadjigol, S Shabani, VF Jafari, A Barlow, GG Qiao, NM O’Brien-Simpson

2025-04-30

The fast emergence of bacteria resistance has already threatened global health, and immediate action is required before the emerge..

Real-time, label-free detection and identification of bacteria through non-invasive optical imaging

Farah Qazi, Rajni Verma, Connagh E Redmond, Asma Khalid, Neil M O'Brien-Simpson, Snjezana Tomljenovic-Hanic

2024-01-01

Currently, traditional and newer molecular and mass spectrometry techniques of identifying bacteria from biological samples requir..

Label-free macrophage phenotype classification using machine learning methods

T Hourani, A Perez-Gonzalez, K Khoshmanesh, R Luwor, AA Achuthan, S Baratchi, NM O’Brien-Simpson, A Al-Hourani

2023-12-01

Macrophages are heterogeneous innate immune cells that are functionally shaped by their surrounding microenvironment. Diverse macr..

The effect of tailing lipidation on the bioactivity of antimicrobial peptides and their aggregation tendency: Special Issue: Emerging Investigators

B Lin, A Hung, W Singleton, KK Darmawan, R Moses, B Yao, H Wu, A Barlow, MA Sani, AJ Sloan, MA Hossain, JD Wade, Y Hong, NM O'Brien-Simpson, W Li

2023-08-01

Antimicrobial peptides (AMPs) are potentially powerful alternatives to conventional antibiotics in combating multidrug resistance,..

The overview of antimicrobial peptide-coated implants against oral bacterial infections

Z Sun, L Ma, X Sun, AJ Sloan, NM O'Brien-Simpson, W Li

2023-06-01

Dental implants are the most common therapeutic approach for resolving tooth loss and damage. Despite technical advances in treatm..

Directed chemical dimerisation enhances the antibacterial activity of the antimicrobial peptide MSI-78(4–20)

Rong Li, Thomas NG Handley, Wenyi Li, Neil M O’Brien-Simpson, Mohammed Akhter Hossain, John D Wade

2023-05-31

Antimicrobial resistance (AMR) is on the rise, leading to 700 000 deaths worldwide in 2020. Antimicrobial peptides (AMPs) are ant..

The ‘Danse Macabre’—Neutrophils the Interactive Partner Affecting Oral Cancer Outcomes

S Hadjigol, BA Shah, NM O’Brien-Simpson

2022-06-16

Over the past few decades, tremendous advances in the prevention, diagnosis, and treatment of cancer have taken place. However for..

Star-Peptide Polymers are Multi-Drug-Resistant Gram-Positive Bacteria Killers

W Li, S Hadjigol, AR Mazo, J Holden, J Lenzo, SJ Shirbin, A Barlow, S Shabani, T Huang, EC Reynolds, GG Qiao, NM O'Brien-Simpson

2022-06-08

Antibiotic resistance in bacteria, especially Gram-positive bacteria like Staphylococcus aureus, is gaining considerable momentum ..

Evaluation of Potential DnaK Modulating Proline-Rich Antimicrobial Peptides Identified by Computational Screening

TNG Handley, W Li, NG Welch, NM O’Brien-Simpson, MA Hossain, JD Wade

2022-04-13

The day is rapidly approaching where current antibiotic therapies will no longer be effective due to the development of multi-drug..

Systematic comparison of activity and mechanism of antimicrobial peptides against nosocomial pathogens

B Lin, A Hung, R Li, A Barlow, W Singleton, T Matthyssen, MA Sani, MA Hossain, JD Wade, NM O'Brien-Simpson, W Li

2022-03-05

The World Health Organisation has deemed several multi-drug resistant (MDR) nosocomial bacterial pathogens to be of significant th..

Enhancing proline-rich antimicrobial peptide action by homodimerization: Influence of bifunctional linker

W Li, F Lin, A Hung, A Barlow, MA Sani, R Paolini, W Singleton, J Holden, MA Hossain, F Separovic, NM O'Brien-Simpson, JD Wade

2022-02-28

Antimicrobial peptides (AMPs) are host defense peptides, and unlike conventional antibiotics, they possess potent broad spectrum a..

The Potential of Modified and Multimeric Antimicrobial Peptide Materials as Superbug Killers

T Matthyssen, W Li, JA Holden, JC Lenzo, S Hadjigol, NM O’Brien-Simpson

2022-01-10

Antimicrobial peptides (AMPs) are found in nearly all living organisms, show broad spectrum antibacterial activity, and can modula..

The Potential of Calcium Phosphate Nanoparticles as Adjuvants and Vaccine Delivery Vehicles

Z Sun, W Li, JC Lenzo, JA Holden, MJ McCullough, AJ O’Connor, NM O’Brien-Simpson

2021-12-22

Vaccination is one of the most efficacious and cost-effective ways to protect people from infectious diseases and potentially canc..

Tumor Associated Macrophages: Origin, Recruitment, Phenotypic Diversity, and Targeting

T Hourani, JA Holden, W Li, JC Lenzo, S Hadjigol, NM O’Brien-Simpson

2021-12-20

The tumor microenvironment (TME) is known to have a strong influence on tumorigenesis, with various components being involved in t..

Cationic Antimicrobial Peptides Are Leading the Way to Combat Oropathogenic Infections

B Lin, R Li, TNG Handley, JD Wade, W Li, NM O'Brien-Simpson

2021-11-12

Oral dental infections are one of the most common diseases affecting humans, with caries and periodontal disease having the highes..

Recent Applications of Aggregation Induced Emission Probes for Antimicrobial Peptide Studies

T Luu, W Li, NM O'Brien-Simpson, Y Hong

2021-05-03

Antimicrobial peptides (AMPs) are being intensively investigated as they are considered promising alternatives to antibiotics wher..

Chemically modified and conjugated antimicrobial peptides against superbugs

W Li, F Separovic, NM O'Brien-Simpson, JD Wade

2021-04-21

Antimicrobial resistance (AMR) is one of the greatest threats to human health that, by 2050, will lead to more deaths from bacteri..

Human glucose-dependent insulinotropic polypeptide (GIP) is an antimicrobial adjuvant re-sensitising multidrug-resistant Gram-negative bacteria

DMM Jaradat, N Al-Karablieh, BHM Zaarer, W Li, KKY Saleh, AJ Rasras, S Abu-Romman, NM O'Brien-Simpson, JD Wade

2021-03-01

Increasing antibiotic resistance in Gram-negative bacteria has mandated the development of both novel antibiotics and alternative ..

Multifunctional Antimicrobial Polypeptide-Selenium Nanoparticles Combat Drug-Resistant Bacteria

T Huang, JA Holden, EC Reynolds, DE Heath, NM O'Brien-Simpson, AJ O'Connor

2020-12-16

Antibiotic-resistant bacteria are a severe threat to human health. The World Health Organization's Global Antimicrobial Surveillan..

(Re)Defining the Proline-Rich Antimicrobial Peptide Family and the Identification of Putative New Members

NG Welch, W Li, MA Hossain, F Separovic, NM O'Brien-Simpson, JD Wade

2020-12-01

As we rapidly approach a post-antibiotic era in which multi-drug resistant bacteria are ever-pervasive, antimicrobial peptides (AM..

Antimicrobial nanoparticle coatings for medical implants: Design challenges and prospects

X Li, T Huang, DE Heath, NM Obrien-Simpson, AJ Oconnor

2020-11-01

Microbial colonization, infection, and biofilm formation are major complications in the use of implants and are the predominant ri..