HIV Infection: Immunobiology and Vaccine design
Grant number: 299907
This program application seeks to draw on the skills of a world leading group of Australian researchers to bring novel HIV vaccine designs to clinical trials, improve vaccine design and create new opportunities for commercialisation. The Chief Investigators, Prof David Cooper, Prof Peter Doherty (Nobel Prize winner), A-Prof Stephen Kent and Prof Ian Ramshaw, have achieved major scientific developments including: innovative collaborative clinical trials, cutting edge research in T cell immunology, the establishment of the only PC3-level nonhuman primate facility in the Southern hemisphere, T cell immunogenicity of the DNA-viral vector prime-boost vaccine regimens and ground-breaking research ..View full description
Related publications (22)
Narrowed TCR diversity for immunised mice challenged with recombinant influenza A-HIV Env(311-320) virus
Tania Cukalac, Jessica M Moffat, Vanessa Venturi, Miles P Davenport, Peter C Doherty, Stephen J Turner, John Stambas
Understanding CD8+ T cell responses generated by live virus vectors is critical for the rational design of next generation HIV CTL..
Induction of HIV-1 subtype B and AE-specific neutralizing antibodies in mice and macaques with DNA prime and recombinant gp140 protein boost regimens
Rob J Center, Adam K Wheatley, Shahan M Campbell, Adriana J Gaeguta, Viv Peut, Sheilajen Alcantara, Carly Siebentritt, Stephen J Kent, Damian FJ Purcell
We developed highly expressing clade B and AE DNA and envelope protein (Env) vaccines for evaluation in mice and macaques as DNA p..
Evaluation of Recombinant Influenza Virus-Simian Immunodeficiency Virus Vaccines in Macaques
Amy Sexton, Robert De Rose, Jeanette C Reece, Sheilajen Alcantara, Liyen Loh, Jessica M Moffat, Karen Laurie, Aeron Hurt, Peter C Doherty, Stephen J Turner, Stephen J Kent, John Stambas
There is an urgent need for human immunodeficiency virus (HIV) vaccines that induce robust mucosal immunity. Influenza A viruses (..
Transience of MHC Class I-restricted antigen presentation after influenza A virus infection
Justine D Mintern, Sammy Bedoui, Gayle M Davey, Jessica M Moffat, Peter C Doherty, Stephen J Turner
Antigen expressed as MHC Class I glycoprotein (pMHCI) complexes on dendritic cells is the primary driver of CD8(+) T cell clonal e..
Peripheral NKT Cells in Simian Immunodeficiency Virus-Infected Macaques
Caroline S Fernandez, Angela C Chan, Konstantinos Kyparissoudis, Robert De Rose, Dale I Godfrey, Stephen J Kent
NKT cells are a specialized population of T lymphocytes that have an increasingly recognized role in immunoregulation, including c..
Protection afforded by live attenuated SIV is associated with rapid killing kinetics of CTLs
Erik Rollman, Rosemarie D Mason, Jie Lin, Andrew G Brooks, Stephen J Kent
BACKGROUND: Live attenuated SIV vaccines are highly efficacious, but how they mediate protection is poorly understood. A feature o..
Low pre-infection levels and loss of central memory CD4 T cells may predict rapid progression in SIV-infected pigtail macaques
Rosemarie D Mason, Robert De Rose, Nabila Seddiki, Anthony D Kelleher, Stephen J Kent
CD4+ T lymphocyte subsets are targeted to different degrees by SIV infection. We studied central memory, effector memory, naïve, a..
Limited maintenance of vaccine-induced simian immunodeficiency virus-specific CD8 T-cell receptor clonotypes after virus challenge
Miranda Z Smith, Tedi E Asher, Vanessa Venturi, Miles P Davenport, Daniel C Douek, David A Price, Stephen J Kent
T-cell receptors (TCRs) govern the specificity, efficacy, and cross-reactivity of CD8 T cells. Here, we studied CD8 T-cell clonoty..
Evaluation of recombinant Kunjin replicon SIV vaccines for protective efficacy in macaques
Stephen J Kent, Robert De Rose, Vlad V Mokhonov, Ekaterina I Mokhonova, Caroline S Fernandez, Sheilajen Alcantara, Erik Rollman, Rosemarie D Mason, Liyen Loh, Viv Peut, Jeanette C Reece, Xiang Ju Wang, Kim M Wilson, Andreas Suhrbier, Alexander Khromykh
Persistent gag-specific T cell immunity would be a useful component of an effective HIV vaccine. The Flavivirus Kunjin replicon wa..
A minimal uORF within the HIV-1 vpu leader allows efficient translation initiation at the downstream env AUG
Jorg Krummheuer, Adam T Johnson, Ilona Hauber, Susanne Kammler, Jenny L Anderson, Joachim Hauber, Damian FJ Purcell, Heiner Schaal
The HIV-1 Vpu and Env proteins are translated from 16 alternatively spliced bicistronic mRNA isoforms. Translation of HIV-1 mRNAs ..
Both linear and discontinuous ribosome scanning are used for translation initiation from bicistronic human immunodeficiency virus type 1 env mRNAs
Jenny L Anderson, Adam T Johnson, Jane L Howard, Damian FJ Purcell
Human immunodeficiency virus type 1 (HIV-1) generates 16 alternatively spliced isoforms of env mRNA that contain the same overlapp..
In vivo fitness costs of different gag CD8 T-cell escape mutant simian-human immunodeficiency viruses for macaques
Liyen Loh, C Jane Batten, Janka Petravic, Miles P Davenport, Stephen J Kent
The kinetics of immune escape and reversion depend upon the efficiency of CD8 cytotoxic T lymphocytes (CTL) and the fitness cost o..
The testis and epididymis are productively infected by SIV and SHIV in juvenile macaques during the post-acute stage of infection
Miranda Shehu-Xhilaga, Stephen Kent, Jane Batten, Sarah Ellis, Joel Van der Meulen, Moira O'Bryan, Paul U Cameron, Sharon R Lewin, Mark P Hedger
BACKGROUND: Little is known about the progression and pathogenesis of HIV-1 infection within the male genital tract (MGT), particu..
Comparative efficacy of subtype AE simian-human immunodeficiency virus priming and boosting vaccines in pigtail macaques
Robert De Rose, C Jane Batten, Miranda Z Smith, Caroline S Fernandez, Viv Peut, Scott Thomson, Ian A Ramshaw, Barbara EH Coupar, David B Boyle, Vanessa Venturi, Miles P Davenport, Stephen J Kent
Vaccination against AIDS is hampered by great diversity between human immunodeficiency virus (HIV) strains. Heterologous B-subtype..
Mucosally-administered human-simian immunodeficiency virus DNA and fowlpoxvirus-based recombinant vaccines reduce acute phase viral replication in macaques following vaginal challenge with CCR5-tropic SHIVSF162P3
SJ Kent, CJ Dale, C Ranasinghe, I Stratov, R De Rose, S Chea, DC Montefiori, S Thomson, IA Ramshaw, BEH Coupar, DB Boyle, M Law, KM Wilson, AJ Ramsay
Further advances are required in understanding protection from AIDS by T cell immunity across mucosal sites of virus transmission...
Phenotypic and kinetic analysis of effective simian-human immunodeficiency virus-specific T cell responses in DNA- and fowlpox virus-vaccinated macaques
I Stratov, CJ Dale, SJ Kent
Although T cell immunity is important in the control of HIV-1 infection, the characteristics of effective HIV-specific T cell resp..
Rapid viral escape at an immunodominant simian-human immunodeficiency virus cytotoxic T-lymphocyte epitope exacts a dramatic fitness cost
CS Fernandez, I Stratov, R De Rose, K Walsh, CJ Dale, MZ Smith, MB Agy, SL Hu, K Krebs, DI Watkins, DH O'Connor, MP Davenport, SJ Kent
Escape from specific T-cell responses contributes to the progression of human immunodeficiency virus type 1 (HIV-1) infection. T-c..
Granulocyte contamination dramatically inhibits spot formation in AIDS virus-specific ELISpot assays: Analysis and strategies to ameliorate
R De Rose, EL Taylor, MG Law, PH van der Meide, SJ Kent
The interferon-gamma (IFNgamma) ELISpot assay has become the most critical tool for HIV vaccine evaluation. External factors affec..
Analysis of pigtail macaque major histocompatibility complex class I molecules presenting immunodominant simian immunodeficiency virus epitopes
MZ Smith, CJ Dale, R De Rose, I Stratov, CS Fernandez, AG Brooks, J Weinfurter, K Krebs, C Riek, DI Watkins, DH O'Connor, SJ Kent
Successful human immunodeficiency virus (HIV) vaccines will need to induce effective T-cell immunity. We studied immunodominant si..