Understanding and Exploiting Bacterial Sulfatases

Grant number: DP0449625

Abstract

Bacterial sulfatases participate in environmental nutrient cycling and are implicated in bacterial pathogenesis mechanisms. These enzymes catalyze the hydrolysis of sulfate esters and possess an unusual posttranslational active-site modification where a cysteine residue is oxidized to formylglycine. We will study the mechanism of these enzymes in detail and design inhibitors that exploit the reactivity of this aminoacid. This work has significance because of application to areas that include the treatment of cancer and bacterial infections. Additionally, we will clone novel carbohydrate sulfatases from the heparin-degrading bacterium Flavobacterium heparinum. These sulfatases will have use i..

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Related publications (4)

Direct evidence for ArO-S bond cleavage upon inactivation of Pseudomonas aeruginosa arylsulfutase by aryl sulfamates

P Bojarová, E Denehy, I Walker, K Loft, DP De Souza, LWL Woo, BVL Potter, MJ McConville, SJ Williams

2008-03-03

Pseudomonas aeruginosa arylsulfatase catalyses the cleavage of aryl sulfates and is an excellent model for human estrone sulfatase..

Electronic structure of the sulfonyl and phosphonyl groups: A computational and crystallographic study

E Denehy, JM White, SJ Williams

2007-10-15

A computational and X-ray crystallographic investigation of the electronic and geometric structures of a range of sulfonyl (-SO<in..

Galactose-derived phosphonate analogues as potential inhibitors of phosphatidylinositol biosynthesis in mycobacteria

Z Dinev, CT Gannon, C Egan, JA Watt, MJ McConville, SJ Williams

2007-01-01

Galactose-based phosphonate analogues of myo-inositol-1-phosphate and phosphatidylinositol have been synthesized from methyl β-d- ..

Use of click chemistry to define the substrate specificity of Leishmania β-1,2-mannosyltransferases

P Van Der Peet, CT Gannon, I Walker, Z Dinev, M Angelin, S Tam, JE Ralton, MJ McConville, SJ Williams

2006-09-01

Leishmania spp. are human pathogens that utilize a novel β-7,2-mannan as their major carbohydrate reserve material. We describe a ..

University of Melbourne Researchers

Spencer Williams