Structure-function and domain minimization of insulin-like peptide 3, a novel member of the insulin superfamily
Grant number: 350245
Insulin-like peptide 3 (INSL3) is a peptide hormone that is structurally similar to insulin. It is produced in both the testes and the ovaries. In the male, one of its primary roles is to initiate testes descent during fetal development via a direct action on the gubernaculum ligament. Failure of INSL3 action either directly or due to receptor malfunction causes cryptorchidism (undescended testes), one of the most common congenital defects. In the female, INSL3 is implicated in follicle selection. More recent evidence shows that the peptide has clear roles in modulating male and female germ cell maturation. These effects indicate that agonists and antagonists of INSL3 have potential as speci..View full description
Related publications (10)
General method for selective labelling of double-chain cysteine-rich peptides with a lanthanide chelate via solid-phase synthesis
Fazel Shabanpoor, Frances Separovic, John D Wade
The use of lanthanides in preference to radioisotopes as probes for various biological assays has gained enormous popularity. The ..
Role of the intra-A-chain disulfide bond of insulin-like peptide 3 in binding and activation of its receptor, RXFP2
Suode Zhang, Richard A Hughes, Ross AD Bathgate, Fazel Shabanpoor, M Akhter Hossain, Feng Lin, Bianca van Lierop, Andrea J Robinson, John D Wade
INSL3 is a member of the insulin-IGF-relaxin superfamily and plays a key role in male fetal development and in adult germ cell mat..
Effect of helix-promoting strategies on the biological activity of novel analogues of the B-chain of INSL3
Fazel Shabanpoor, Richard A Hughes, Suode Zhang, Ross AD Bathgate, Sharon Layfield, Mohammed Akhter Hossain, Geoffrey W Tregear, Frances Separovic, John D Wade
Insulin-like 3 (INSL3) is a novel circulating peptide hormone that is produced by testicular Leydig cells and ovarian thecal and l..
Development of Lanthanide-Labeled Human INSL3 as an Alternative Probe to Radioactively Labeled INSL3 for Use in Bioassays
Fazel Shabanpoor, Richard A Hughes, Ross AD Bathgate, Frances Separovic, John D Wade
The use of radioisotope-labeled peptides and proteins in bioassays is diminishing as a consequence of the emergence of fluorescent..
Dimerization and Negative Cooperativity in the Relaxin Family Peptide Receptors
Angela Manegold Svendsen, Milka Vrecl, Louise Knudsen, Anders Heding, John D Wade, Ross AD Bathgate, Pierre De Meyts, Jane Nohr
Peptides of the relaxin family bind to the relaxin family peptide receptors or RXFPs, members of the G-protein-coupled receptor (G..
Evaluation of Relaxin's Antifibrotic Action by SELDI-TOF Mass Spectrometry-Based Profiling of Relaxin Knockout Mice, a Model of Progressive Fibrosis
Eleni Giannakis, Mary Macris, Linda Chan, Geoffrey W Tregear, Chrishan S Samuel, John D Wade
Surface-enhanced laser desorption ionization-time-of-flight mass spectrometry was employed to identify potential biomarkers for ea..
The Chemical Synthesis of Relaxin and Related Peptides A Historical Perspective
John D Wade, Feng Lin, M Akhter Hossain, Fazel Shabanpoor, Suode Zhang, Geoffrey W Tregear
Successful methods for the chemical assembly of insulin-like peptides allow the detailed study of their structure and function rel..
Negative cooperativity in H2 relaxin binding to a dimeric relaxin family peptide receptor 1
Angela Manegold Svendsen, Anna Zalesko, Julie Konig, Milka Vrecl, Anders Heding, Jesper Boggild Kristensen, John D Wade, Ross AD Bathgate, Pierre De Meyts, Jane Nohr
H2 relaxin, a member of the insulin superfamily, binds to the G-protein-coupled receptor RXFP1 (relaxin family peptide 1), a recep..