tRNA Synthetases as Drug Targets in Plasmodium Falciparum

A dual-targeted aminoacyl-tRNA synthetase in Plasmodium falciparum charges cytosolic and apicoplast tRNACys

JS Pham, R Sakaguchi, LM Yeoh, NS De Silva, GI Mcfadden, YM Hou, SA Ralph

2014-03-15

Plasmodium parasites possess two endosymbiotic organelles: a mitochondrion and a relict plastid called the apicoplast. To accommod..

Aminoacyl-tRNA synthetases as drug targets in eukaryotic parasites

JS Pham, KL Dawson, KE Jackson, EE Lim, CFA Pasaje, KEC Turner, SA Ralph

2014-01-01

Aminoacyl-tRNA synthetases are central enzymes in protein translation, providing the charged tRNAs needed for appropriate construc..

An FtsH Protease Is Recruited to the Mitochondrion of Plasmodium falciparum

A Tanveer, SM Allen, KE Jackson, M Charan, SA Ralph, S Habib

2013-09-13

The two organelles, apicoplast and mitochondrion, of the malaria parasite Plasmodium falciparum have unique morphology in liver an..

Electron tomography of Plasmodium falciparum merozoites reveals core cellular events that underpin erythrocyte invasion

E Hanssen, C Dekiwadia, DT Riglar, M Rug, L Lemgruber, AF Cowman, M Cyrklaff, M Kudryashev, F Frischknecht, J Baum, SA Ralph

2013-09-01

Summary: Erythrocyte invasion by merozoites forms of the malaria parasite is a key step in the establishment of human malaria dise..

Recycling factors for ribosome disassembly in the apicoplast and mitochondrion of Plasmodium falciparum

A Gupta, SS Mir, KE Jackson, EE Lim, P Shah, A Sinha, MI Siddiqi, SA Ralph, S Habib

2013-06-01

The reduced genomes of the apicoplast and mitochondrion of the malaria parasite Plasmodium falciparum are actively translated and ..

Cryo-electron tomography reveals four-membrane architecture of the Plasmodium apicoplast

L Lemgruber, M Kudryashev, C Dekiwadia, DT Riglar, J Baum, H Stahlberg, SA Ralph, F Frischknecht

2013-01-21

Background: The apicoplast is a plastid organelle derived from a secondary endosymbiosis, containing biosynthetic pathways essenti..

Determination of protein subcellular localization in apicomplexan parasites

BJ Woodcroft, PJ McMillan, C Dekiwadia, L Tilley, SA Ralph

2012-12-01

Parasites from the phylum Apicomplexa include causative agents of serious diseases including malaria (Plasmodium spp.) and toxopla..

In silico prediction of antimalarial drug target candidates

P Ludin, B Woodcroft, SA Ralph, P Mäser

2012-12-01

The need for new antimalarials is persistent due to the emergence of drug resistant parasites. Here we aim to identify new drug ta..

Malaria Parasite Signal Peptide Peptidase is an ER-Resident Protease Required for Growth but not for Invasion

DS Marapana, DW Wilson, ES Zuccala, CD Dekiwadia, JG Beeson, SA Ralph, J Baum

2012-11-01

The establishment of parasite infection within the human erythrocyte is an essential stage in the development of malaria disease. ..

Subcompartmentalisation of Proteins in the Rhoptries Correlates with Ordered Events of Erythrocyte Invasion by the Blood Stage Malaria Parasite

ES Zuccala, AM Gout, C Dekiwadia, DS Marapana, F Angrisano, L Turnbull, DT Riglar, KL Rogers, CB Whitchurch, SA Ralph, TP Speed, J Baum

2012-09-25

Host cell infection by apicomplexan parasites plays an essential role in lifecycle progression for these obligate intracellular pa..

Investigation of the Plasmodium falciparum food vacuole through inducible expression of the chloroquine resistance transporter (PfCRT)

F Ehlgen, JS Pham, T de Koning-Ward, AF Cowman, SA Ralph

2012-06-13

Haemoglobin degradation during the erythrocytic life stages is the major function of the food vacuole (FV) of Plasmodium falciparu..

Spatial localisation of actin filaments across developmental stages of the malaria parasite

F Angrisano, DT Riglar, A Sturm, JC Volz, MJ Delves, ES Zuccala, L Turnbull, C Dekiwadia, MA Olshina, DS Marapana, W Wong, V Mollard, CH Bradin, CJ Tonkin, PW Gunning, SA Ralph, CB Whitchurch, RE Sinden, AF Cowman, GI McFadden

2012-02-28

Actin dynamics have been implicated in a variety of developmental processes during the malaria parasite lifecycle. Parasite motili..

Dual targeting of aminoacyl-tRNA synthetases to the apicoplast and cytosol in Plasmodium falciparum

KE Jackson, JS Pham, M Kwek, NS De Silva, SM Allen, CD Goodman, GI McFadden, L Ribas de Pouplana, SA Ralph

2012-02-01

The causative agent of malaria, Plasmodium, possesses three translationally active compartments: the cytosol, the mitochondrion an..

Organellar proteomics reveals hundreds of novel nuclear proteins in the malaria parasite Plasmodium falciparum

SC Oehring, BJ Woodcroft, S Moes, J Wetzel, O Dietz, A Pulfer, C Dekiwadia, P Maeser, C Flueck, K Witmer, NMB Brancucci, I Niederwieser, P Jenoe, SA Ralph, TS Voss

2012-01-01

Background: The post-genomic era of malaria research provided unprecedented insights into the biology of Plasmodium parasites. Due..

Protein translation in Plasmodium parasites

KE Jackson, S Habib, M Frugier, R Hoen, S Khan, JS Pham, LRD Pouplana, M Royo, MAS Santos, A Sharma, SA Ralph

2011-10-01

The protein translation machinery of the parasite Plasmodium is the target of important anti-malarial drugs, and encompasses many ..

Plasmodium falciparum merozoite invasion is inhibited by antibodies that target the PfRH2a and b binding domains

T Triglia, L Chen, S Lopaticki, C Dekiwadia, DT Riglar, AN Hodder, SA Ralph, J Baum, AF Cowman

2011-06-01

Plasmodium falciparum, the causative agent of the most severe form of malaria in humans invades erythrocytes using multiple ligand..

Super-resolution dissection of coordinated events during malaria parasite invasion of the human erythrocyte

DT Riglar, D Richard, DW Wilson, MJ Boyle, C Dekiwadia, L Turnbull, F Angrisano, DS Marapana, KL Rogers, CB Whitchurch, JG Beeson, AF Cowman, SA Ralph, J Baum

2011-01-20

Erythrocyte invasion by the merozoite is an obligatory stage in Plasmodium parasite infection and essential to malaria disease pro..

Isolation of viable Plasmodium falciparum merozoites to define erythrocyte invasion events and advance vaccine and drug development

MJ Boyle, DW Wilson, JS Richards, DT Riglar, KKA Tetteh, DJ Conway, SA Ralph, J Baum, JG Beeson

2010-08-10

During blood-stage infection by Plasmodium falciparum, merozoites invade RBCs. Currently there is limited knowledge of cellular an..

Drug target prediction and prioritization: Using orthology to predict essentiality in parasite genomes

MA Doyle, RB Gasser, BJ Woodcroft, RS Hall, SA Ralph

2010-04-03

Background: New drug targets are urgently needed for parasites of socio-economic importance. Genes that are essential for parasite..

Novel vacuoles in Toxoplasma

GG Van Dooren, SA Ralph

2010-01-01