Deciphering the cellular defenses against aggregating proteins in human disease
Grant number: DP120102763 | Funding period: 2012 - 2014
Cells have inbuilt defences for coping with proteins that bend into abnormal sticky shapes that form toxic clusters. In many diseases, including Huntington's, the clusters severely damage nerve cells. This project will identify the genes and mechanisms cells use to protect themselves from toxic clusters, which could provide new therapeutic targets.
Related publications (5)
Polyalanine expansions drive a shift into alpha-helical clusters without amyloid-fibril formation
Saskia Polling, Angelique R Ormsby, Rebecca J Wood, Kristie Lee, Cheryl Shoubridge, James N Hughes, Paul Q Thomas, Michael DW Griffin, Andrew F Hill, Quill Bowden, Till Boecking, Danny M Hatters
Polyglutamine (polyGln) expansions in nine human proteins result in neurological diseases and induce the proteins' tendency to for..
Misfolded Polyglutamine, Polyalanine, and Superoxide Dismutase 1 Aggregate via Distinct Pathways in the Cell
Saskia Polling, Yee-Foong Mok, Yasmin M Ramdzan, Bradley J Turner, Justin J Yerbury, Andrew F Hill, Danny M Hatters
Protein aggregation into intracellular inclusions is a key feature of many neurodegenerative disorders. A common theme has emerged..
A Platform to View Huntingtin Exon 1 Aggregation Flux in the Cell Reveals Divergent Influences from Chaperones hsp40 and hsp70
Angelique R Ormsby, Yasmin M Ramdzan, Yee-Foong Mok, Kristijan D Jovanoski, Danny M Hatters
Our capacity for tracking how misfolded proteins aggregate inside a cell and how different aggregation states impact cell biology ..
Tyrosine 416 Is Phosphorylated in the Closed, Repressed Conformation of c-Src
Sevgi Irtegun, Rebecca J Wood, Angelique R Ormsby, Terrence D Mulhern, Danny M Hatters
c-Src kinase activity is regulated by phosphorylation of Y527 and Y416. Y527 phosphorylation stabilizes a closed conformation, whi..
Pulse shape analysis (PulSA) to track protein translocalization in cells by flow cytometry: applications for polyglutamine aggregation.
Yasmin M Ramdzan, Rebecca Wood, Danny M Hatters
Pulse shape analysis (PulSA) is a flow cytometry-based method that can be used to study protein localization patterns in cells. Ex..