Discovering the function and structure of RIO kinases - toward new nematocides
Grant number: 1044022 | Funding period: 2013 - 2015
This project is focused on: high quality fundamental molecular science, contributing to national objectives, including the development of novel and innovative scientific concepts and international collaborations; consolidating links between basic and applied research; enhancing the skills-base in molecular biology and global visibility of Australian science.
Related publications (38)
Functional genomic exploration reveals that Ss-RIOK-1 is essential for the development and survival of Strongyloides stercoralis larvae
Wang Yuan, Huan Zhou, James B Lok, Weiqiang Lei, Siyuan He, Robin B Gasser, Rui Zhou, Rui Fang, Yanqin Zhou, Junlong Zhao, Min Hu
Protein kinase RIOK-1 is a non-ribosomal factor essential for rRNA cleavage and ribosome small subunit maturation. It is encoded i..
Deguelin exerts potent nematocidal activity via the mitochondrial respiratory chain
Sarah Preston, Pasi K Korhonen, Laurent Mouchiroud, Matteo Cornaglia, Sean L McGee, Neil D Young, Rohan A Davis, Simon Crawford, Cameron Nowell, Brendan RE Ansell, Gillian M Fisher, Katherine T Andrews, Bill CH Chang, Martin AM Gijs, Paul W Sternberg, Johan Auwerx, Jonathan Baell, Andreas Hofmann, Abdul Jabbar, Robin B Gasser
As a result of limited classes of anthelmintics and an over-reliance on chemical control, there is a great need to discover new co..
Genetic diversity in the C-terminus of merozoite surface protein 1 among Plasmodium knowlesi isolates from Selangor and Sabah Borneo, Malaysia
Nan Jiun Yap, Xiang Ting Goh, Anson V Koehler, Timothy William, Tsin Wen Yeo, Indra Vythilingam, Robin B Gasser, Yvonne AL Lim
Plasmodium knowlesi, a malaria parasite of macaques, has emerged as an important parasite of humans. Despite the significance of P..
Structural and developmental expression of Ss-riok-2, an RIO protein kinase encoding gene of Strongyloides stercoralis
Wei-Qiang Lei, James B Lok, Wang Yuan, Yue-Zhou Zhang, Jonathan D Stoltzfus, Robin B Gasser, Si-Yuan He, Huan Zhou, Rui Zhou, Jun-Long Zhao, Min Hu
RIO kinases are essential atypical protein kinases in diverse prokaryotic and eukaryotic organisms, playing significant roles in y..
Assessing the anthelmintic activity of pyrazole-5-carboxamide derivatives against Haemonchus contortus
Yaqing Jiao, Sarah Preston, Hongjian Song, Abdul Jabbar, Yuxiu Liu, Jonathan Baell, Andreas Hofmann, Dana Hutchinson, Tao Wang, Anson V Koehler, Gillian M Fisher, Katherine T Andrews, Benoit Laleu, Michael J Palmer, Jeremy N Burrows, Timothy NC Wells, Qingmin Wang, Robin B Gasser
BACKGROUND: In this study, we tested five series of pyrazole-5-carboxamide compounds (n = 55) for activity against parasitic stage..
Enzyme characteristics of pathogen-specific trehalose-6-phosphate phosphatases
Megan Cross, Siji Rajan, Janine Chekaiban, Jake Saunders, Chloe Hamilton, Jeong-Sun Kim, Mark J Coster, Robin B Gasser, Andreas Hofmann
Owing to the key role of trehalose in pathogenic organisms, there has recently been growing interest in trehalose metabolism for t..
Metabolic profiling and in vitro assessment of anthelmintic fractions of Picria fel-terrae Lour
Rasika Kumarasingha, Avinash V Karpe, Sarah Preston, Tiong-Chia Yeo, Diana SL Lim, Chu-Lee Tu, Jennii Luu, Kaylene J Simpson, Jillian M Shaw, Robin B Gasser, David J Beale, Paul D Morrison, Enzo A Palombo, Peter R Boag
Anthelmintic resistance is widespread in gastrointestinal nematode populations, such that there is a consistent need to search for..
Screening of the 'Pathogen Box' identifies an approved pesticide with major anthelmintic activity against the barber's pole worm
Sarah Preston, Yaqing Jiao, Abdul Jabbar, Sean L McGee, Benoit Laleu, Paul Willis, Timothy NC Wells, Robin B Gasser
There is a substantial need to develop new medicines against parasitic diseases via public-private partnerships. Based on high thr..
Two benzimidazole resistance-associated SNPs in the isotype-1 beta-tubulin gene predominate in Haemonchus contortus populations from eight regions in China
Zongze Zhang, Robin B Gasser, Xin Yang, Fangyuan Yin, Guanghui Zhao, Min Bao, Baoliang Pan, Weiyi Huang, Chunren Wang, Fengcai Zou, Yanqin Zhou, Junlong Zhao, Rui Fang, Min Hu
Haemonchus contortus is one of the most important parasitic nematodes of small ruminants around the world, particularly in tropica..
Population structure of Haemonchus contortus from seven geographical regions in China, determined on the basis of microsatellite markers
Fangyuan Yin, Robin B Gasser, Facai Li, Min Bao, Weiyi Huang, Fengcai Zou, Guanghui Zhao, Chunren Wang, Xin Yang, Yanqin Zhou, Junlong Zhao, Rui Fang, Min Hu
BACKGROUND: Studying genetic variation within and among Haemonchus contortus populations can inform some aspects of this parasite'..
Organometallic Derivatization of the Nematocidal Drug Monepantel Leads to Promising Antiparasitic Drug Candidates
Jeannine Hess, Malay Patra, Loganathan Rangasamy, Sandro Konatschnig, Olivier Blacque, Abdul Jabbar, Patrick Mac, Erik M Jorgensen, Robin B Gasser, Gilles Gasser
The discovery of novel drugs against animal parasites is in high demand due to drug-resistance problems encountered around the wor..
Synthesis, Characterization, and Biological Activity of Ferrocenyl Analogues of the Anthelmintic Drug Monepantel
Jeannine Hess, Malay Patra, Vanessa Pierroz, Bernhard Spingler, Abdul Jabbar, Stefano Ferrari, Robin B Gasser, Gilles Gasser
There is major demand for the development of structurally new anti-infectives using innovative approaches to circumvent multidrug ..
More parasiticmyositis cases in humans in Australia, and the definition of genetic markers for the causative agents as a basis for molecular diagnosis
Anson V Koehler, David M Spratt, Robert Norton, Sanchia Warren, Belinda McEwan, Ravindra Urkude, Suresh Murthy, Thomas Robertson, Naomi McCallum, Fiona Parsonson, Richard S Bradbury, Robin B Gasser
Since 1998, there have been six reported human cases of myositis in Australia, attributable to infection with the nematode Haycock..
Detection of cryptic species of Rugopharynx (Nematoda: Strongylida) from the stomachs of Australian macropodid marsupials
Neil B Chilton, Florence Huby-Chilton, Anson V Koehler, Robin B Gasser, Ian Beveridge
Sequences of the internal transcribed spacers of nuclear ribosomal DNA (ITS-1 and ITS-2) were determined for species of the genus ..
Time-Course Study of the Transcriptome of Peripheral Blood Mononuclear Cells (PBMCs) from Sheep Infected with Fasciola hepatica
Cristian A Alvarez Rojas, Jean-Pierre Scheerlinck, Brendan RE Ansell, Ross S Hall, Robin B Gasser, Aaron R Jex
Fasciola hepatica is a parasitic trematode that infects a wide range of mammalian hosts, including livestock and humans, in temper..
Selenophene and thiophene-core estrogen receptor ligands that inhibit motility and development of parasitic stages of Haemonchus contortus
Sarah Preston, Junjie Luo, Yuezhou Zhang, Abdul Jabbar, Simon Crawford, Jonathan Baell, Andreas Hofmann, Min Hu, Hai-Bing Zhou, Robin B Gasser
BACKGROUND: Parasitic worms represent a substantial disease burden in animals and humans worldwide. The control of parasitic round..
Cryptosporidium and Giardia taxa in faecal samples from animals in catchments supplying the city of Melbourne with drinking water (2011 to 2015)
Anson V Koehler, Shane R Haydon, Aaron R Jex, Robin B Gasser
BACKGROUND: In a long-term program to monitor pathogens in water catchments serving the City of Melbourne in the State of Victoria..
MicroRNAs of Toxocara canis and their predicted functional roles
Guangxu Ma, Yongfang Luo, Honghong Zhu, Yongli Luo, Pasi K Korhonen, Neil D Young, Robin B Gasser, Rongqiong Zhou
BACKGROUND: Toxocara canis is the causative agent of toxocariasis of humans and other animals. This parasitic nematode (roundworm)..
Anthelmintic activity of selected ethno-medicinal plant extracts on parasitic stages of Haemonchus contortus
Rasika Kumarasingha, Sarah Preston, Tiong-Chia Yeo, Diana SL Lim, Chu-Lee Tu, Enzo A Palombo, Jillian M Shaw, Robin B Gasser, Peter R Boag
BACKGROUND: Parasitic roundworms (nematodes) cause substantial morbidity and mortality in livestock animals globally, and consider..
Phylogenetic relationships of species of the oesophageal parasitic nematode genera Cyclostrongylus and Spirostrongylus (Strongyloidea: Chabertiidae: Cloacininae) with their wallaby hosts (Marsupialia: Macropodidae)
Neil B Chilton, Florence Huby-Chilton, Anson V Koehler, Robin B Gasser, Ian Beveridge
A phylogeny for seven species of Cyclostrongylus and the monotypic genus Spirostrongylus (Nematoda: Chabertiidae), all highly host..
The complement of family M1 aminopeptidases of Haemonchus contortus - Biotechnological implications
Namitha Mohandas, Neil D Young, Abdul Jabbar, Pasi K Korhonen, Anson V Koehler, Ross S Hall, Min Hu, Andreas Hofmann, Robin B Gasser
Although substantial research has been focused on the 'hidden antigen' H11 of Haemonchus contortus as a vaccine against haemonchos..
Dictyocaulus viviparus genome, variome and transcriptome elucidate lungworm biology and support future intervention
Samantha N McNulty, Christina Struebe, Bruce A Rosa, John C Martin, Rahul Tyagi, Young-Jun Choi, Qi Wang, Kymberlie Hallsworth Pepin, Xu Zhang, Philip Ozersky, Richard K Wilson, Paul W Sternberg, Robin B Gasser, Makedonka Mitreva
The bovine lungworm, Dictyocaulus viviparus (order Strongylida), is an important parasite of livestock that causes substantial eco..
Molecular characterization of the Haemonchus contortus phosphoinositide-dependent protein kinase-1 gene (Hc-pdk-1)
Fa-Cai Li, Robin B Gasser, James B Lok, Pasi K Korhonen, Li He, Wen-Da Di, Fang-Yuan Yin, Rui Zhou, Yan-Qin Zhou, Jun-Long Zhao, Min Hu
BACKGROUND: Phosphoinositide-dependent protein kinase-1 (PDK-1), which functions downstream of phosphoinositide 3-kinase (AGE-1) a..
Reconstruction of the insulin-like signalling pathway of Haemonchus contortus
Namitha Mohandas, Min Hu, Andreas J Stroehlein, Neil D Young, Paul W Sternberg, James B Lok, Robin B Gasser
BACKGROUND: In the present study, we reconstructed the insulin/insulin-like growth factor 1 signalling (IIS) pathway for Haemonchu..
Molecular analysis of Cryptosporidium from cattle from five states of Peninsular Malaysia
Nan Jiun Yap, Anson V Koehler, Janine Ebner, Tiong Kai Tan, Yvonne AL Lim, Robin B Gasser
Despite the importance of the cattle industry in Malaysia, there are very few studies of the diversity and public health significa..
Phylogenomic and biogeographic reconstruction of the Trichinella complex
Pasi K Korhonen, Edoardo Pozio, Giuseppe La Rosa, Bill CH Chang, Anson V Koehler, Eric P Hoberg, Peter R Boag, Patrick Tan, Aaron R Jex, Andreas Hofmann, Paul W Sternberg, Neil D Young, Robin B Gasser
Trichinellosis is a globally important food-borne parasitic disease of humans caused by roundworms of the Trichinella complex. Ext..
Practical and low cost whole-organism motility assay: A step-by-step protocol
Sarah Preston, Abdul Jabbar, Cameron Nowell, Anja Joachim, Baerbel Ruttkowski, Tony Cardno, Andreas Hofmann, Robin B Gasser
Here, we provide a step-by-step protocol for a practical and low cost whole-organism assay for the screening of chemical compounds..
Assessment of the nematocidal activity of metallocenyl analogues of monepantel
Jeannine Hess, Malay Patra, Abdul Jabbar, Vanessa Pierroz, Sandro Konatschnig, Bernhard Spingler, Stefano Ferrari, Robin B Gasser, Gilles Gasser
In this study, we present the design, synthesis, characterization and biological evaluation of structurally new ferrocenyl and rut..
Panel docking of small-molecule libraries - Prospects to improve efficiency of lead compound discovery
Pakornwit Sarnpitak, Prashant Mujumdar, Paul Taylor, Megan Cross, Mark J Coster, Alain-Dominique Gorse, Mikhail Krasavin, Andreas Hofmann
Computational docking as a means to prioritise small molecules in drug discovery projects remains a highly popular in silico scree..
Investigating the Role of RIO Protein Kinases in Caenorhabditis elegans
Tasha K Mendes, Stevan Novakovic, Greta Raymant, Sonja E Bertram, Reza Esmaillie, Saravanapriah Nadarajan, Bert Breugelmans, Andreas Hofmann, Robin B Gasser, Monica P Colaiacovo, Peter R Boag
RIO protein kinases (RIOKs) are a relatively conserved family of enzymes implicated in cell cycle control and ribosomal RNA proces..
SOX9 Duplication Linked to Intersex in Deer
Regina Kropatsch, Gabriele Dekomien, Denis A Akkad, Wanda M Gerding, Elisabeth Petrasch-Parwez, Neil D Young, Janine Altmueller, Peter Nuernberg, Robin B Gasser, Joerg T Epplen
A complex network of genes determines sex in mammals. Here, we studied a European roe deer with an intersex phenotype that was con..