P2X7 mediated phagocytosis of apoptotic cells: a common mechanism underlies neurological and eye disorders

P2X7-mediated alteration of membrane fluidity is associated with the late stages of age-related macular degeneration.

Candace Drysdale, Keunha Park, Kirstan A Vessey, Xin Huang, Emily Caruso, Yihan Li, Joelyn Wong, James S Wiley, Erica Fletcher, Robyn H Guymer, Ben J Gu

2022-12-01

We have shown deficits in monocyte phagocytosis from patients with age-related macular degeneration (AMD). Cell membrane fluidity ..

Leukocyte surface biomarkers implicate deficits of innate immunity in sporadic Alzheimer's disease

Xin Huang, Yihan Li, Christopher Fowler, James D Doecke, Yen Ying Lim, Candace Drysdale, Vicky Zhang, Keunha Park, Brett Trounson, Kelly Pertile, Rebecca Rumble, John W Pickering, Robert A Rissman, Floyd Sarsoza, Sara Abdel-Latif, Yong Lin, Vincent Dore, Victor Villemagne, Christopher C Rowe, Jurgen Fripp

2022-11-09

INTRODUCTION: Blood-based diagnostics and prognostics in sporadic Alzheimer's disease (AD) are important for identifying at-risk i..

Identification of Leukocyte Surface P2X7 as a Biomarker Associated with Alzheimer's Disease

Yihan Li, Xin Huang, Christopher Fowler, Yen Y Lim, Simon M Laws, Noel Faux, James D Doecke, Brett Trounson, Kelly Pertile, Rebecca Rumble, Vincent Dore, Victor L Villemagne, Christopher C Rowe, James S Wiley, Paul Maruff, Colin L Masters, Ben J Gu

2022-07-01

Alzheimer's disease (AD) has shown altered immune responses in the periphery. We studied P2X7 (a proinflammatory receptor and a sc..

Flow Cytometry Identifies an Early Stage of Platelet Apoptosis Produced by Agonists of the P2X1 and P2X7 Receptors

Joelyn Wong, Ben J Gu, Harry Teoh, Malgorzata Krupa, Mastura Monif, Mark Slee, James S Wiley

2022-01-20

Platelets express P2X1 receptors and our data also show the expression of P2X7 receptors. We studied the role of both receptors in..

Deficits in Monocyte Function in Age Related Macular Degeneration: A Novel Systemic Change Associated With the Disease

Ben J Gu, Xin Huang, Pavan K Avula, Emily Caruso, Candace Drysdale, Kirstan A Vessey, Amber Ou, Christopher Fowler, Tian-Hua Liu, Yong Lin, Adam Horton, Colin L Masters, James S Wiley, Robyn H Guymer, Erica L Fletcher

2021-03-17

Age-related macular degeneration (AMD) is characterized by the accumulation of debris in the posterior eye. In this study we evalu..

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells

Hannah C Leeson, Tailoi Chan-Ling, Michael D Lovelace, Jeremy D Brownlie, Michael W Weible, Ben J Gu

2019-04-01

Live-cell flow cytometry is increasingly used among cell biologists to quantify biological processes in a living cell culture. Thi..

P2X7 Receptors Regulate Phagocytosis and Proliferation in Adult Hippocampal and SVZ Neural Progenitor Cells: Implications for Inflammation in Neurogenesis

Hannah C Leeson, Maria A Kasherman, Tailoi Chan-Ling, Michael D Lovelace, Jeremy C Brownlie, Kelly M Toppinen, Ben J Gu, Michael W Weible

2018-11-01

Identifying the signaling mechanisms that regulate adult neurogenesis is essential to understanding how the brain may respond to n..

The scavenger activity of the human P2X7 receptor differs from P2X7 pore function by insensitivity to antagonists, genetic variation and sodium concentration: Relevance to inflammatory brain diseases

Amber Ou, Ben J Gu, James S Wiley

2018-04-01

Activation of P2X7 receptors is widely recognised to initiate proinflammatory responses. However P2X7 also has a dual function as ..

Purinergic receptors P2RX4 and P2RX7 in familial multiple sclerosis

A Dessa Sadovnick, Ben J Gu, Anthony L Traboulsee, Cecily Q Bernales, Mary Encarnacion, Irene M Yee, Maria G Criscuoli, Xin Huang, Amber Ou, Carol J Milligan, Steven Petrou, James S Wiley, Carles Vilarino-Guell

2017-06-01

Genetic variants in the purinergic receptors P2RX4 and P2RX7 have been shown to affect susceptibility to multiple sclerosis (MS). ..

Innate phagocytosis by peripheral blood monocytes is altered in Alzheimer's disease

Ben J Gu, Xin Huang, Amber Ou, Alan Rembach, Christopher Fowler, Pavan K Avula, Adam Horton, James D Doecke, Victor L Villemagne, S Lance Macaulay, Paul Maruff, Erica L Fletcher, Robyn Guymer, James S Wiley, Colin L Masters

2016-09-01

Sporadic Alzheimer's disease (AD) is characterised by the deposition and accumulation of specific protein aggregates. Failure of c..

A rare P2X7 variant Arg307Gln with absent pore formation function protects against neuroinflammation in multiple sclerosis

Ben J Gu, Judith Field, Sebastien Dutertre, Amber Ou, Trevor J Kilpatrick, Jeannette Lechner-Scott, Rodney Scott, Rodney Lea, Bruce V Taylor, Jim Stankovich, Helmut Butzkueven, Melissa Gresle, Simon M Laws, Steven Petrou, Sabine Hoffjan, Denis A Akkad, Colin A Graham, Stanley Hawkins, Anna Glaser, Sahl Khalid Bedri

2015-10-01

Multiple sclerosis (MS) is a chronic relapsing-remitting inflammatory disease of the central nervous system characterized by oligo..

P2X7 IS AN ARCHAIC SCAVENGER RECEPTOR RECOGNIZING APOPTOTIC NEUROBLASTS IN EARLY HUMAN NEUROGENESIS

2015-05-02

Shear stress modulates endothelial KLF2 through activation of P2X4

R Sathanoori, F Rosi, BJ Gu, JS Wiley, CE Mueller, B Olde, D Erlinge

2015-03-01

Vascular endothelial cells that are in direct contact with blood flow are exposed to fluid shear stress and regulate vascular home..

P2X7 Receptors Mediate Innate Phagocytosis by Human Neural Precursor Cells and Neuroblasts

Michael D Lovelace, Ben J Gu, Steven S Eamegdool, Michael W Weible, James S Wiley, David G Allen, Tailoi Chan-Ling

2015-02-01

During early human neurogenesis there is overproduction of neuroblasts and neurons accompanied by widespread programmed cell death..

A Quantitative Method for Measuring Innate Phagocytosis by Human Monocytes Using Real-Time Flow Cytometry

Ben J Gu, Chun Sun, Stephen Fuller, Kristen K Skarratt, Steven Petrou, James S Wiley

2014-04-01

Phagocytosis is central to immunity however a rapid and standardized method is much needed for quantitative assessment of the phag..

A rare functional haplotype of the P2RX4 and P2RX7 genes leads to loss of innate phagocytosis and confers increased risk of age-related macular degeneration

Ben J Gu, Paul N Baird, Kirstan A Vessey, Kristen K Skarratt, Erica L Fletcher, Stephen J Fuller, Andrea J Richardson, Robyn H Guymer, James S Wiley

2013-04-01

Age-related macular degeneration (AMD) is a leading cause of blindness in Western countries and is diagnosed by the clinical appea..