How Does Redox Cycling Drive the Metabolism of the Essential Metals Iron and Copper?

The crystal structure of the CopC protein from Pseudomonas fluorescens reveals amended classifications for the CopC protein family

SR Udagedara, CJK Wijekoon, Z Xiao, AG Wedd, MJ Maher

2019-06-01

The bacterial CopC family of proteins are periplasmic copper binding proteins that act in copper detoxification. These proteins co..

Molecular Mechanisms of Glutaredoxin Enzymes: Versatile Hubs for Thiol–Disulfide Exchange between Protein Thiols and Glutathione

Z Xiao, S La Fontaine, AI Bush, AG Wedd

2019-01-18

The tripeptide glutathione (GSH) and its oxidized form glutathione disulfide (GSSG) constitute a key redox couple in cells. In par..

The Human Amyloid Precursor Protein Binds Copper Ions Dominated by a Picomolar-Affinity Site in the Helix-Rich E2 Domain

TR Young, TL Pukala, R Cappai, AG Wedd, Z Xiao

2018-07-17

A manifestation of Alzheimer's disease (AD) is the aggregation in the brain of amyloid β (Aβ) peptides derived from the amyloid pr..

Evaluation of Cu(i) binding to the E2 domain of the amyloid precursor protein-a lesson in quantification of metal binding to proteins via ligand competition

TR Young, AG Wedd, Z Xiao

2018-01-01

The extracellular domain E2 of the amyloid precursor protein (APP) features a His-rich metal-binding site (denoted as the M1 site)..

Glutaredoxins employ parallel monothiol-dithiol mechanisms to catalyze thiol-disulfide exchanges with protein disulfides

AA Ukuwela, AI Bush, AG Wedd, Z Xiao

2018-01-01

Glutaredoxins (Grxs) are a family of glutathione (GSH)-dependent thiol-disulfide oxidoreductases. They feature GSH-binding sites t..

Reduction potentials of protein disulfides and catalysis of glutathionylation and deglutathionylation by glutaredoxin enzymes

AA Ukuwela, AI Bush, AG Wedd, Z Xiao

2017-11-15

Glutaredoxins (Grxs) are a class of GSH (glutathione)-dependent thiol–disulfide oxidoreductase enzymes. They use the cellular redo..

Molecular Aspects of a Robust Assay for Ferroxidase Function of Ceruloplasmin

L Cortes, BR Roberts, AG Wedd, Z Xiao

2017-05-01

Ceruloplasmin (Cp) is one of the most complex multicopper oxidase enzymes and plays an essential role in the metabolism of iron in..

Copper ATPase CopA from Escherichia coli: Quantitative Correlation between ATPase Activity and Vectorial Copper Transport

Chathuri JK Wijekoon, Saumya R Udagedara, Roland L Knorr, Rumiana Dimova, Anthony G Wedd, Zhiguang Xiao

2017-03-29

Cu-ATPases are membrane copper transporters present in all kingdoms of life. They play a central role in Cu homeostasis by pumping..

Copper binding and redox chemistry of the Ab16 peptide and its variants: insights into determinants of copper-dependent reactivity†

N Yako, TR Young, JM Cottam Jones, CA Hutton, AG Wedd, Z Xiao

2017-03-01

The metal-binding sites of Ab peptides are dictated primarily by the coordination preferences of the metal ion. Consequently, Cu(I..

Evaluation of employing poly-lysine tags versus poly-histidine tags for purification and characterization of recombinant copper-binding proteins

CJK Wijekoon, AA Ukuwela, AG Wedd, Z Xiao

2016-09-01

Quantitative characterization of metalloproteins at molecular and atomic levels generally requires tens of milligrams of highly pu..

The functional roles of the three copper sites associated with the methionine-rich insert in the multicopper oxidase CueO from E. coli

L Cortes, AG Wedd, Z Xiao

2015-05-01

CueO from Escherichia coli is a multicopper oxidase (MCO) involved in copper tolerance under aerobic conditions. It features the f..

CopC protein from pseudomonas fluorescens SBW25 features a conserved novel high-affinity Cu(II) binding site

CJK Wijekoon, TR Young, AG Wedd, Z Xiao

2015-03-16

Copper homeostasis in the bacterium Pseudomonas fluorescens SBW25 appears to be mediated mainly via chromosomal cue and cop system..

A set of robust fluorescent peptide probes for quantification of Cu(ii) binding affinities in the micromolar to femtomolar range

TR Young, CJK Wijekoon, B Spyrou, PS Donnelly, AG Wedd, Z Xiao

2015-03-01

Reliable quantification of copper binding affinities and identification of the binding sites provide a molecular basis for an unde..

An integrated study of the affinities of the Aβ16 peptide for Cu(I) and Cu(II): Implications for the catalytic production of reactive oxygen species

TR Young, A Kirchner, AG Wedd, Z Xiao

2014-03-01

A new fluorescent probe Aβ16wwa based upon the Aβ16 peptide has been developed with two orders of magnitude greater fluorescence i..

Redox sulfur chemistry of the copper chaperone Atox1 is regulated by the enzyme glutaredoxin 1, the reduction potential of the glutathione couple GSSG/2GSH and the availability of Cu(i)

J Brose, S La Fontaine, AG Wedd, Z Xiao

2014-01-01

Glutaredoxins have been characterised as enzymes regulating the redox status of protein thiols via cofactors GSSG/GSH. However, su..