UNTANGLING THE PLANT GOLGI APPARATUS: FUNCTIONAL PROTEOMICS TO UNDERSTAND PLANT CELL WALL BIOSYNTHESIS.
Grant number: FT130101165 | Funding period: 2014 - 2019
The plant cell wall determines plant morphology and structure. It is also a major factor in food quality, and it is used as forage and is the raw material for a range of industries. A significant proportion of the cell wall is synthesised in a poorly studied cellular compartment known as the Golgi apparatus. This project intends to exploit unique isolation and analytical techniques in conjunction to further profile and characterise this structure in order to uncover new information about the complex interplay of components involved in plant cell wall biosynthesis. This information will be used to support approaches to manipulate cell walls to produce plant biomass optimised for agricultural ..View full description
Related publications (22)
The companion of cellulose synthase 1 confers salt tolerance through a Tau-like mechanism in plants
Christopher Kesten, Arndt Wallmann, Rene Schneider, Heather E McFarlane, Anne Diehl, Ghazanfar Abbas Khan, Barth-Jan van Rossum, Edwin R Lampugnani, Witold G Szymanski, Nils Cremer, Peter Schmieder, Kristina L Ford, Florian Seiter, Joshua L Heazlewood, Clara Sanchez-Rodriguez, Hartmut Oschkinat, Staffan Persson
Microtubules are filamentous structures necessary for cell division, motility and morphology, with dynamics critically regulated b..
The Three Members of the Arabidopsis Glycosyltransferase Family 92 are Functional beta-1,4-Galactan Synthases
Berit Ebert, Devon Birdseye, April JM Liwanag, Tomas Laursen, Emilie A Rennie, Xiaoyuan Guo, Michela Catena, Carsten Rautengarten, Solomon H Stonebloom, Pawel Gluza, Venkataramana R Pidatala, Mathias CF Andersen, Roshan Cheetamun, Jenny C Mortimer, Joshua L Heazlewood, Antony Bacic, Mads H Clausen, William GT Willats, Henrik V Scheller
Pectin is a major component of primary cell walls and performs a plethora of functions crucial for plant growth, development and p..
Three UDP-xylose transporters participate in xylan biosynthesis by conveying cytosolic UDP-xylose into the Golgi lumen in Arabidopsis
Xianhai Zhao, Nian Liu, Na Shang, Wei Zeng, Berit Ebert, Carsten Rautengarten, Qing-Yin Zeng, Huiling Li, Xiaoyang Chen, Cherie Beahan, Antony Bacic, Joshua L Heazlewood, Ai-Min Wu
UDP-xylose (UDP-Xyl) is synthesized by UDP-glucuronic acid decarboxylases, also termed UDP-Xyl synthases (UXSs). The Arabidopsis g..
Absolute Quantitation of In Vitro Expressed Plant Membrane Proteins by Targeted Proteomics (MRM) for the Determination of Kinetic Parameters.
Carsten Rautengarten, Berit Ebert, Joshua L Heazlewood
The purification of a functional soluble protein from biological or in vitro expression systems can be problematic and the enrichm..
Multiple marker abundance profiling: combining selected reaction monitoring and data-dependent acquisition for rapid estimation of organelle abundance in subcellular samples
Cornelia M Hooper, Tim J Stevens, Anna Saukkonen, Ian R Castleden, Pragya Singh, Gregory W Mann, Bertrand Fabre, Jun Ito, Michael J Deery, Kathryn S Lilley, Christopher J Petzold, A Harvey Millar, Joshua L Heazlewood, Harriet T Parsons
Measuring changes in protein or organelle abundance in the cell is an essential, but challenging aspect of cell biology. Frequentl..
The elaborate route for UDP-arabinose delivery into the Golgi of plants
Carsten Rautengarten, Devon Birdseye, Sivakumar Pattathil, Heather E McFarlane, Susana Saez-Aguayo, Ariel Orellana, Staffan Persson, Michael G Hahn, Henrik V Scheller, Joshua L Heazlewood, Berit Ebert
In plants, L-arabinose (Ara) is a key component of cell wall polymers, glycoproteins, as well as flavonoids, and signaling peptide..
Enrichment of Golgi Membranes from Triticum aestivum (Wheat) Seedlings
Wei Zeng, Berit Ebert, Harriet T Parsons, Carsten Rautengarten, Antony Bacic, Joshua L Heazlewood
The Golgi apparatus is an essential component in the plant secretory pathway. The enrichment of Golgi membranes from plant tissue ..
Influence of light and nitrogen on the photosynthetic efficiency in the C-4 plant Miscanthus x giganteus
Jian-Ying Ma, Wei Sun, Nuria K Koteyeva, Elena Voznesenskaya, Samantha S Stutz, Anthony Gandin, Andreia M Smith-Moritz, Joshua L Heazlewood, Asaph B Cousins
There are numerous studies describing how growth conditions influence the efficiency of C4 photosynthesis. However, it remains unc..
UUAT1 Is a Golgi-Localized UDP-Uronic Acid Transporter That Modulates the Polysaccharide Composition of Arabidopsis Seed Mucilage
Susana Saez-Aguayo, Carsten Rautengarten, Henry Temple, Dayan Sanhueza, Troy Ejsmentewicz, Omar Sandoval-Ibanez, Daniela Donas, Juan Pablo Parra-Rojas, Berit Ebert, Arnaud Lehner, Jean-Claude Mollet, Paul Dupree, Henrik V Scheller, Joshua L Heazlewood, Francisca C Reyes, Ariel Orellana
UDP-glucuronic acid (UDP-GlcA) is the precursor of many plant cell wall polysaccharides and is required for production of seed muc..
We Are Not Alone: The iMOP Initiative and Its Roles in a Biology- and Disease-Driven Human Proteome Project
Andreas Tholey, Nicolas L Taylor, Joshua L Heazlewood, Einoke Bendixen
Mapping of the human proteome has advanced significantly in recent years and will provide a knowledge base to accelerate our under..
The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development
Carsten Rautengarten, Berit Ebert, Lifeng Liu, Solomon Stonebloom, Andreia M Smith-Moritz, Markus Pauly, Ariel Orellana, Henrik Vibe Scheller, Joshua L Heazlewood
Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify..
Free-Flow Electrophoresis of Plasma Membrane Vesicles Enriched by Two-Phase Partitioning Enhances the Quality of the Proteome from Arabidopsis Seedlings
Roberto de Michele, Heather E McFarlane, Harriet T Parsons, Miranda J Meents, Jeemeng Lao, Susana M Gonzalez Fernandez-Nino, Christopher J Petzold, Wolf B Frommer, A Lacey Samuels, Joshua L Heazlewood
The plant plasma membrane is the interface between the cell and its environment undertaking a range of important functions related..
Characterization of protein N-glycosylation by tandem mass spectrometry using complementary fragmentation techniques
Kristina L Ford, We Zeng, Joshua L Heazlewood, Antony Bacic
The analysis of post-translational modifications (PTMs) by proteomics is regarded as a technically challenging undertaking. While ..
Proteome profile of the endomembrane of developing coleoptiles from switchgrass (Panicum virgatum)
Jeemeng Lao, Manoj K Sharma, Rita Sharma, Susana M Gonzalez Fernandez-Nino, Jeremy Schmutz, Pamela C Ronald, Joshua L Heazlewood, Benjamin Schwessinger
The cost-effective production of biofuels from lignocellulosic material will likely require manipulation of plant biomass, specifi..
Identification and Characterization of a Golgi-Localized UDP-Xylose Transporter Family from Arabidopsis
Berit Ebert, Carsten Rautengarten, Xiaoyuan Guo, Guangyan Xiong, Solomon Stonebloom, Andreia M Smith-Moritz, Thomas Herter, Leanne Jade G Chan, Paul D Adams, Christopher J Petzold, Markus Pauly, William GT Willats, Joshua L Heazlewood, Henrik Vibe Scheller
Most glycosylation reactions require activated glycosyl donors in the form of nucleotide sugars to drive processes such as posttra..