Structure and activity determination of membrane-active peptides
Grant number: DP140102127 | Funding period: 2014 - 2016
Membrane-active peptides, such as antimicrobial and amyloid (Ab) peptides, play an important role in disease. With the growth of antibiotic resistance and increase in Alzheimer’s disease, which is epitomised by plaques of Ab, new drugs are required. Although Ab is toxic in neuronal cell cultures and disrupts cell membranes, the mechanism is unknown. Antimicrobial peptides that target bacterial membranes have evolved as a defence mechanism against infection and, since membranes show little genetic adaptation, could be drug candidates. Model membranes will be developed to elucidate the mechanism of action and key molecular features that determine affinity for membrane lipids of an antimicrobia..View full description
Related publications (16)
Utilizing magnetic resonance techniques to study membrane interactions of amyloid peptides
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The cleavage of the amyloid precursor protein by β- and γ-secretases is a key event in Alzheimer's disease. A fusion protein was c..
Effect of dimerized melittin on gastric cancer cells and antibacterial activity
Elaheh Jamasbi, Sasidharan S Lucky, Wenyi Li, Mohammed Akhter Hossain, Ponnampalam Gopalakrishnakone, Frances Separovic
Melittin is the peptide toxin found in bee venom and is effective against cancer cells. To enhance its activity, a branched dimeri..
Interaction of N-terminal peptide analogues of the Na ,K -ATPase with membranes
Nguyen Khoa, Alvaro Garcia, Marc-Antoine Sani, Dil Diaz, Vikas Dubey, Daniel Clayton, Giovanni Dal Poggetto, Flemming Cornelius, Richard J Payne, Frances Separovic, Himanshu Khandelia, Ronald J Clarke
The Na+,K+-ATPase, which is present in the plasma membrane of all animal cells, plays a crucial role in maintaining the Na+ and K+..
Covalent conjugation of cationic antimicrobial peptides with a beta-lactam antibiotic core
Wenyi Li, Neil M O'Brien-Simpson, James A Holden, Laszlo Otvos, Eric C Reynolds, Frances Separovic, Mohammed Akhter Hossain, John D Wade
To combat the serious issue of increasing global antibiotic resistance, new antimicrobial therapies are urgently required. As one ..
Phosphorylation of a full length amyloid-beta peptide modulates its amyloid aggregation, cell binding and neurotoxic properties
Elaheh Jamasbi, Frances Separovic, Mohammed Akhter Hossain, Giuseppe Donato Ciccotosto
Amyloid beta peptide (Aβ) is the major protein component of the amyloid plaques that are present in the brains of Alzheimer's dise..
The efficient synthesis and purification of amyloid-beta(1-42) using an oligoethylene glycol-containing photocleavable lysine tag
John A Karas, Asif Noor, Christine Schieber, Timothy U Connell, Frances Separovic, Paul S Donnelly
A short, monodisperse oligoethylene glycol-containing photocleavable lysine tag was developed to facilitate the efficient purifica..
C-Terminal Modification and Multimerization Increase the Efficacy of a Proline-Rich Antimicrobial Peptide
Wenyi Li, Neil M O'Brien-Simpson, Shenggen Yao, Julien Tailhades, Eric C Reynolds, Raymond M Dawson, Laszlo Otvos, Mohammed Akhter Hossain, Frances Separovic, John D Wade
Two series of branched tetramers of the proline-rich antimicrobial peptide (PrAMP), Chex1-Arg20, were prepared to improve antibact..
Interaction of the antimicrobial peptides caerin 1.1 and aurein 1.2 with intact bacteria by H-2 solid-state NMR
Marwa Laadhari, Alexandre A Arnold, Andree E Gravel, Frances Separovic, Isabelle Marcotte
Nuclear magnetic resonance (NMR) is commonly used to probe the effect of antimicrobial agents on bacterial membranes using model m..
Atomic force microscopy of bacteria reveals the mechanobiology of pore forming peptide action
Anna Mularski, Jonathan J Wilksch, Eric Hanssen, Richard A Strugnell, Frances Separovic
Time-resolved AFM images revealed that the antimicrobial peptide (AMP) caerin 1.1 caused localised defects in the cell walls of ly..
Amyloid Beta (A beta) Peptide and Factors that Play Important Roles in Alzheimer's Disease
Elaheh Jamasbi, John D Wade, Frances Separovic, Mohammed A Hossain
Amyloid beta peptide (Aβ) is recognised as a main feature of Alzheimer's disease (AD). Increasing evidence suggests that small sol..
Bacteria May Cope Differently from Similar Membrane Damage Caused by the Australian Tree Frog Antimicrobial Peptide Maculatin 1.1
Marc-Antoine Sani, Sonia Troeira Henriques, Daniel Weber, Frances Separovic
Maculatin 1.1 (Mac1) is an antimicrobial peptide from the skin of Australian tree frogs and is known to possess selectivity toward..