The lipidomics of cell fate
Grant number: FT140100047 | Funding period: 2015 - 2018
This project aims to dissect the roles of lipids in cell fate. The study of lipids, or lipidomics, is an emerging and exciting area of biological science. The fundamental roles of lipids in development remain vastly understudied. This project will look at reprogramming of somatic cells into stem cells, their pluripotency and differentiation. This will be complemented with studies in the zebrafish, which permits the direct study of cell fate in vivo. This approach is a powerful way to unlock major events involved in development and to unmask the roles of lipids in these fundamental mechanisms.
Related publications (22)
Transcriptomic Profiling of Human Pluripotent Stem Cell-derived Retinal Pigment Epithelium over Time.
Grace E Lidgerwood, Anne Senabouth, Casey JA Smith-Anttila, Vikkitharan Gnanasambandapillai, Dominik C Kaczorowski, Daniela Amann-Zalcenstein, Erica L Fletcher, Shalin H Naik, Alex W Hewitt, Joseph E Powell, Alice Pébay
Human pluripotent stem cell (hPSC)-derived progenies are immature versions of cells, presenting a potential limitation to the accu..
Comparative performance of the BGI and Illumina sequencing technology for single-cell RNA-sequencing.
Anne Senabouth, Stacey Andersen, Qianyu Shi, Lei Shi, Feng Jiang, Wenwei Zhang, Kristof Wing, Maciej Daniszewski, Samuel W Lukowski, Sandy SC Hung, Quan Nguyen, Lynn Fink, Anthony Beckhouse, Alice Pébay, Alex W Hewitt, Joseph E Powell
The libraries generated by high-throughput single cell RNA-sequencing (scRNA-seq) platforms such as the Chromium from 10× Genomics..
PSEN1 Delta E9, APPswe, and APOE4 Confer Disparate Phenotypes in Human iPSC-Derived Microglia
Henna Konttinen, Mauricio E Castro Cabral-da-Silva, Sohvi Ohtonen, Sara Wojciechowski, Anastasia Shakirzyanova, Simone Caligola, Rosalba Giugno, Yevheniia Ishchenko, Damian Hernandez, Mohammad Feroze Fazaludeen, Shaila Eamen, Mireia Gomez Budia, Ilkka Fagerlund, Flavia Scoyni, Paula Korhonen, Nadine Huber, Annakaisa Haapasalo, Alex W Hewitt, James Vickers, Grady C Smith
Bio-engineering a tissue flap utilizing a porous scaffold incorporating a human induced pluripotent stem cell-derived endothelial cell capillary network connected to a vascular pedicle
Anne M Kong, Kiryu K Yap, Shiang Y Lim, Diego Marre, Alice Pebay, Yi-wen Gerrand, Jarmon G Lees, Jason A Palmer, Wayne A Morrison, Geraldine M Mitchell
Tissue flaps are used to cover large/poorly healing wounds, but involve complex surgery and donor site morbidity. In this study a ..
Single-Cell Profiling Identifies Key Pathways Expressed by iPSCs Cultured in Different Commercial Media
Maciej Daniszewski, Nguyen Quan, Hun S Chy, Vikrant Singh, Duncan E Crombie, Tejal Kulkarni, Helena H Liang, Priyadharshini Sivakumaran, Grace E Lidgerwood, Damien Hernandez, Alison Conquest, Louise A Rooney, Sophie Chevalier, Stacey B Andersen, Anne Senabouth, James C Vickers, David A Mackey, Jamie E Craig, Andrew L Laslett, Alex W Hewitt
Human fibroblast and stem cell resource from the Dominantly Inherited Alzheimer Network
Celeste M Karch, Damian Hernandez, Jen-Chyong Wang, Jacob Marsh, Alex W Hewitt, Simon Hsu, Joanne Norton, Denise Levitch, Tamara Donahue, Wendy Sigurdson, Bernardino Ghetti, Martin Farlow, Jasmeer Chhatwal, Sarah Berman, Carlos Cruchaga, John C Morris, Randall J Bateman, Alice Pebay, Alison M Goate
Data Descriptor: Transcriptome sequencing and molecular markers discovery in the gonads of Portunus sanguinolentus
Yin Zhang, Guidong Miao, Qingyang Wu, Fan Lin, Cuihong You, Shuqi Wang, Jude Juventus Aweya, Hongyu Ma
Crab culture has gained prominence in the last decade due to the large global market demand for live crabs and crab products. Port..
Role of lysophosphatidic acid in the retinal pigment epithelium and photoreceptors
Grace E Lidgerwood, Andrew J Morris, Alison Conquest, Maciej Daniszewski, Louise A Rooney, Shiang Y Lim, Damian Hernandez, Helena H Liang, Penelope Allen, Paul P Connell, Robyn H Guymer, Alex W Hewitt, Alice Pebay
Mitochondrial fission protein Drp1 inhibition promotes cardiac mesodermal differentiation of human pluripotent stem cells
Ashfaqul Hogue, Priyadharshini Sivakumaran, Simon T Bond, Naomi XY Ling, Anne M Kong, John W Scott, Nadeeka Bandara, Damian Hernandez, Guei-Sheung Liu, Raymond CB Wong, Michael T Ryan, Derek J Hausenloy, Bruce E Kemp, Jonathan S Oakhill, Brian G Drew, Alice Pebay, Shiang Y Lim
Development of a Modular Automated System for Maintenance and Differentiation of Adherent Human Pluripotent Stem Cells
Duncan E Crombie, Maciej Daniszewski, Helena H Liang, Tejal Kulkarni, Fan Li, Grace E Lidgerwood, Alison Conquest, Damian Hernandez, Sandy S Hung, Katherine P Gill, Elisabeth De Smit, Lisa S Kearns, Linda Clarke, Valentin M Sluch, Xitiz Chamling, Donald J Zack, Raymond CB Wong, Alex W Hewitt, Alice Pebay
Participant understanding and recall of informed consent for induced pluripotent stem cell biobanking
Tristan McCaughey, Christine Y Chen, Elisabeth De Smit, Gwyneth Rees, Eva Fenwick, Lisa S Kearns, David A Mackey, Casimir MacGregor, Megan Munsie, Anthony L Cook, Alice Pebay, Alex W Hewitt
Defined Medium Conditions for the Induction and Expansion of Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium
Grace E Lidgerwood, Shiang Y Lim, Duncan E Crombie, Ray Ali, Katherine P Gill, Damian Hernandez, Josh Kie, Alison Conquest, Hayley S Waugh, Raymond CB Wong, Helena H Liang, Alex W Hewitt, Kathryn C Davidson, Alice Pebay