Resistance Exercise Increases Postprandial Muscle Protein Synthesis in Humans

Abstract

PURPOSE: We examined the impact of an acute bout of resistance-type exercise on mixed muscle protein synthesis in the fed state. METHODS: After a standardized breakfast, 10 untrained males completed a single, unilateral lower-limb resistance-type exercise session. A primed, continuous infusion of l-[ring-C6]phenylalanine was combined with muscle biopsy collection from both the exercised (Ex) and the nonexercised (NEx) leg to assess the impact of local muscle contractions on muscle protein synthesis rates after food intake. Western blotting with phosphospecific and pan antibodies was used to determine the phosphorylation status of AMP-activated kinase (AMPK), 4E-binding protein (4E-BP1), mamm..