Genetic analysis of Giardia and Cryptosporidium from people in Northern Australia using PCR-based tools
Janine Ebner, Anson V Koehler, Gemma Robertson, Richard S Bradbury, Aaron R Jex, Shane R Haydon, Melita A Stevens, Robert Norton, Anja Joachim, Robin B Gasser
INFECTION GENETICS AND EVOLUTION | ELSEVIER | Published : 2015
To date, there has been limited genetic study of the gastrointestinal pathogens Giardia and Cryptosporidium in northern parts of Australia. Here, PCR-based methods were used for the genetic characterization of Giardia and Cryptosporidium from 695 people with histories of gastrointestinal disorders from the tropical North of Australia. Genomic DNAs from fecal samples were subjected to PCR-based analyses of regions from the triose phosphate isomerase (tpi), small subunit (SSU) of the nuclear ribosomal RNA and/or the glycoprotein (gp60) genes. Giardia and Cryptosporidium were detected in 13 and four of the 695 samples, respectively. Giardia duodenalis assemblages A and B were found in 4 (31%) a..View full abstract
Awarded by Department of Health (Study Education & Research Committee, SERC), Pathology Queensland, Health Support Queensland, Queensland Government, Australia
Awarded by Victorian Life Sciences Computation Initiative (VLSCI)
Funding from the Department of Health (Study Education & Research Committee, SERC), Pathology Queensland, Health Support Queensland, Queensland Government, Australia (project no. 5030) (G.R. et al.) as well as the Australian Research Council (ARC), the National Health and Medical Research Council (NHMRC) of Australia, Melbourne Water Corporation (R.B.G. et al.) is gratefully acknowledged. Travel support from the University of Veterinary Medicine, Vienna, is gratefully acknowledged (J.E.). This project was also supported by a Victorian Life Sciences Computation Initiative (VLSCI) grant number VR0007 on its Peak Computing Facility at The University of Melbourne, an initiative of the Victorian Government. We thank colleagues at the Microbiology Department of the Townsville Hospital, Queensland for the support and providing information.