Imaging metals in biology: balancing sensitivity, selectivity and spatial resolution
Dominic J Hare, Elizabeth J New, Martin D de Jonge, Gawain McColl
Chemical Society Reviews | ROYAL SOC CHEMISTRY | Published : 2015
Metal biochemistry drives a diverse range of cellular processes associated with development, health and disease. Determining metal distribution, concentration and flux defines our understanding of these fundamental processes. A comprehensive analysis of biological systems requires a balance of analytical techniques that inform on metal quantity (sensitivity), chemical state (selectivity) and location (spatial resolution) with a high degree of certainty. A number of approaches are available for imaging metals from whole tissues down to subcellular organelles, as well as mapping metal turnover, protein association and redox state within these structures. Technological advances in micro- and na..View full abstract
We gratefully acknowledge support from the Australian Research Council and National Health and Medical Research Council, the Victorian Government's Operational Infrastructure Support Program, the Australian Synchrotron, and the UTS Chancellor's Postdoctoral Research Fellowship program. We wish to thank Drs Nicole Jenkins, Simon James and Peter Crouch for critical comments, suggestions and assistance. We also wish to thank Jonas Marnell of Ethix Design for assistance with digital artwork. Due to space limitations, this Tutorial Review could not give appropriate credit to all research in this field, and we wish to acknowledge to the authors of the many great works that were not cited directly.