Growth of confined cancer spheroids: a combined experimental and mathematical modelling approach
D Loessner, JA Flegg, HM Byrne, JA Clements, DW Hutmacher
Integrative Biology | ROYAL SOC CHEMISTRY | Published : 2013
A critical step in the dissemination of ovarian cancer is the formation of multicellular spheroids from cells shed from the primary tumour. The objectives of this study were to apply bioengineered three-dimensional (3D) microenvironments for culturing ovarian cancer spheroids in vitro and simultaneously to build on a mathematical model describing the growth of multicellular spheroids in these biomimetic matrices. Cancer cells derived from human epithelial ovarian carcinoma were embedded within biomimetic hydrogels of varying stiffness and grown for up to 4 weeks. Immunohistochemistry, imaging and growth analyses were used to quantify the dependence of cell proliferation and apoptosis on matr..View full abstract
Awarded by National Health and Medical Research Council (NHMRC) of Australia
Awarded by Australian Research Council's (ARC) Discovery Project funding scheme
Awarded by Queensland University of Technology (QUT)
Support from the National Health and Medical Research Council (NHMRC) of Australia (#553045), the Australian Research Council's (ARC) Discovery Project funding scheme (#DP0878011), an Early Career Research (ECR) Award (#2009001503) from the Queensland University of Technology (QUT) received by DL, a start up grant from QUT for the Chair of Regenerative Medicine, DWH and an Institute of Health and Biomedical Innovation and School of Mathematical Sciences Visiting Research Fellow Award received by HMB are gratefully acknowledged.