Journal article

The Plasmodium translocon of exported proteins component EXP2 is critical for establishing a patent malaria infection in mice

Ming Kalanon, Daniel Bargieri, Angelika Sturm, Kathryn Matthews, Sreejoyee Ghosh, Christopher D Goodman, Sabine Thiberge, Vanessa Mollard, Geoffrey I McFadden, Robert Menard, Tania F de Koning-Ward

Cellular Microbiology | WILEY | Published : 2016

Abstract

Export of most malaria proteins into the erythrocyte cytosol requires the Plasmodium translocon of exported proteins (PTEX) and a cleavable Plasmodium export element (PEXEL). In contrast, the contribution of PTEX in the liver stages and export of liver stage proteins is unknown. Here, using the FLP/FRT conditional mutatagenesis system, we generate transgenic Plasmodium berghei parasites deficient in EXP2, the putative pore-forming component of PTEX. Our data reveal that EXP2 is important for parasite growth in the liver and critical for parasite transition to the blood, with parasites impaired in their ability to generate a patent blood-stage infection. Surprisingly, whilst parasites express..

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Grants

Awarded by National Health and Medical Research Council (NHMRC) of Australia


Funding Acknowledgements

We kindly thank Volker Heussler for providing the EXP1 antibody, Jennifer Pham and Anton Cozijnsen for expert technical assistance and Dejan Bursac for critical reading of the manuscript. This work was supported by grants from the National Health and Medical Research Council (NHMRC) of Australia (Project 1021560) and the OzEMalar Travel Award fund. MK is an Alfred Deakin Postdoctoral Research Fellow and TDK-W is an NHMRC Career Development Fellow.