Polyacrylamide gel-electrophoresis separation of whole-cell proteins.

Abstract

Polyacrylamide gel electrophoresis (PAGE) of whole-cell proteins was first used for differentiation of mycoplasma species by Razin and Rottem (1) The method used was separation through 7.5% acrylamide gels containing 35% acetic acid and 5M urea. However, since the publication of Laemmli’s method for sodium dodecyl sulfate PAGE (SDS-PAGE) (2), most workers have adopted this approach.