Journal article

Obligatory role for B cells in the development of angiotensin II-dependent hypertension

CT Chan, CG Sobey, M Lieu, D Ferens, MM Kett, H Diep, HA Kim, SM Krishnan, CV Lewis, E Salimova, P Tipping, A Vinh, CS Samuel, K Peter, TJ Guzik, TS Kyaw, BH Toh, A Bobik, GR Drummond

Hypertension | Published : 2015

Abstract

Clinical hypertension is associated with raised serum IgG antibodies. However, whether antibodies are causative agents in hypertension remains unknown. We investigated whether hypertension in mice is associated with B-cell activation and IgG production and moreover whether B-cell/IgG deficiency affords protection against hypertension and vascular remodeling. Angiotensin II (Ang II) infusion (0.7 mg/kg per day; 28 days) was associated with (1) a 25% increase in the proportion of splenic B cells expressing the activation marker CD86, (2) an 80% increase in splenic plasma cell numbers, (3) a 500% increase in circulating IgG, and (4) marked IgG accumulation in the aortic adventitia. In B-cell-ac..

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University of Melbourne Researchers

Grants

Awarded by National Health and Medical Research Council of Australia


Awarded by Senior Research Fellowships from National Health and Medical Research Council of Australia


Awarded by Australian Postgraduate Award


Funding Acknowledgements

This work was supported by grants from the National Health and Medical Research Council of Australia (APP1041326). Drs Drummond, Sobey, Samuel, and Peter were supported by Senior Research Fellowships from the National Health and Medical Research Council of Australia (nos APP1006017, APP1079467, APP1041766, and APP1079492, respectively). C.T. Chan was supported by an Australian Postgraduate Award (no. 5131432).