Journal article
Contrasting genetic structure between mitochondrial and nuclear markers in the dengue fever mosquito from Rio de Janeiro: Implications for vector control
G Rašić, R Schama, R Powell, R Maciel-de Freitas, NM Endersby-Harshman, I Filipović, G Sylvestre, RC Máspero, AA Hoffmann
Evolutionary Applications | Published : 2015
DOI: 10.1111/eva.12301
Abstract
Dengue is the most prevalent global arboviral disease that affects over 300 million people every year. Brazil has the highest number of dengue cases in the world, with the most severe epidemics in the city of Rio de Janeiro (Rio). The effective control of dengue is critically dependent on the knowledge of population genetic structuring in the primary dengue vector, the mosquito Aedes aegypti. We analyzed mitochondrial and nuclear genomewide single nucleotide polymorphism markers generated via Restriction-site Associated DNA sequencing, as well as traditional microsatellite markers in Ae. aegypti from Rio. We found four divergent mitochondrial lineages and a strong spatial structuring of mito..
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Funding Acknowledgements
The authors would like to acknowledge Heng Lin Yeap for providing the COI and ND5 amplicon sequences and Araujo SC from the Laboratorio de Biologia Computacional e Sistemas, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro for assisting with the microsatellite genotyping. We are grateful to the Nectar Research Cloud and VIC Node at the University of Melbourne for providing the computing resources for bioinformatics analyses. We would also like to thank Kevin Emerson for valuable comments on the earlier version of the manuscript. This work was funded by a program grant from the National Health and Medical Research Council, a fellowship from the Australian Research Council Australia to AAH, a grant from the Foundation for the National Institutes of Health through the Grand Challenges in Global Health Initiative of the Bill and Melinda Gates Foundation, and a grant from the Rio de Janeiro Science Foundation (FAPERJ) to RS. The funders had no role in study design, data collection, and analysis or preparation of the manuscript.