Journal article

INABILITY TO DETECT CIRCULATING ANTIIDIOTYPIC ANTIBODIES IN HUMAN ANTI-GBM ANTIBODY-MEDIATED DISEASE USING A SPLENIC PFC ASSAY

JA SAVIGE, CM LOCKWOOD

Internal Medicine Journal | ADIS PRESS AUSTRALASIA P/L | Published : 1991

Abstract

Anti-idiotypic antibodies are antibodies that are directed against the variable region of the corresponding antibody molecule and have been postulated to have a regulatory role in the immune response. Circulating anti-idiotypic antibodies have been reported in several antibody-mediated autoimmune diseases. We have established the following detection system for anti-idiotypic antibodies in human anti-glomerular basement membrane (GBM) disease. Spleen cells harvested from BALB/c mice immunised with human GBM were incubated with GBM-coated sheep red blood cells in the presence of guinea pig complement. Each spleen cell that produced anti-GBM antibodies resulted in a surrounding plaque where ant..

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