Journal article

2 ELISAS TO DETECT ANTINEUTROPHIL CYTOPLASM ANTIBODIES (ANCA) IN VARIOUS VASCULITIDES

JA SAVIGE, SP YEUNG, M GALLICCHIO, DJ DAVIES

PATHOLOGY | ROYAL COLLEGE PATHOLOGISTS AUSTRALIA | Published : 1989

Abstract

Anti-neutrophil cytoplasm antibodies (ANCA) are present in the serum of patients with Wegener's granulomatosis, microscopic polyarteritis, and some other small vessel vasculitides. There are at least 2 different anti-neutrophil cytoplasm antibodies identified by their distinctive cytoplasmic staining patterns on indirect immunofluorescence examination. The only antigen identified to date is myeloperoxidase which has a perinuclear distribution on alcohol-fixed neutrophils and monocytes. We have established an ELISA that detects all anti-neutrophil cytoplasm antibodies and one specific for anti-myeloperoxidase antibodies. In the ELISA for anti-neutrophil cytoplasm antibodies, all sera with dif..

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