Molecular analysis of the Huntington's disease gene in New Zealand

Abstract

Aims. To establish and validate a polymerase chain reaction (PCR)-based diagnostic test in New Zealand, which enables the number of CAG repeats present in the Huntingdon's disease (HD) gene to be determined with speed and accuracy. To develop procedures for reporting and counselling probands and families. Methods. The analysis of the CAG repeat region in Huntington's disease and normal chromosomes involved PCR amplification of genomic DNA using either the incorporation of radioactive deoxynucleotides or fluorescent oligonucleotide primers. Results. The molecular analysis of the CAG repeat sequence in the Huntington's disease gene of over 100 New Zealand individuals has been performed. Huntin..