Journal article

LOW-FREQUENCY MOTION IN MEMBRANES THE EFFECT OF CHOLESTEROL AND PROTEINS

BA CORNELL, JB DAVENPORT, F SEPAROVIC

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism | ELSEVIER SCIENCE BV | Published : 1982

Abstract

Nuclear magnetic resonance (NMR) relaxation techniques have been used to study the effect of lipid-protein interactions on the dynamics of membrane lipids. Proton enhanced (PE) 13C-NMR measurements are reported for the methylene chain resonances in red blood cell membranes and their lipid extracts. For comparison similar measurements have been made of phospholipid dispersions containing cholesterol and the polypeptide gramicidin A+. It is found that the spin-lattice relaxation time in the rotating reference frame (T1 rho) is far more sensitive to protein, gramicidin A+ or cholesterol content than is the laboratory frame relaxation time (T1). Based on this data it is concluded that the additi..

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University of Melbourne Researchers