Journal article

CONFORMATION AND ORIENTATION OF GRAMICIDIN-A IN ORIENTED PHOSPHOLIPID-BILAYERS MEASURED BY SOLID-STATE C-13 NMR

BA CORNELL, F SEPAROVIC, AJ BALDASSI, R SMITH

BIOPHYSICAL JOURNAL | BIOPHYSICAL SOCIETY | Published : 1988

Abstract

Three analogues of the helical ionophore gramicidin A have been synthesized with (13)C-labeled carbonyls ((13)C=O) incorporated at either Gly(2), Ala(3), or Val(7). A fourth compound incorporated (13)C at both the carbonyl and alpha-carbon of Gly(2) within the same molecule. These labels were studied using solid-state, proton-enhanced, (13)C nuclear magnetic resonance (NMR) in hydrated dispersions of dimyristoylphosphatidylcholine (DMPC)-gramicidin A. The dispersions were aligned on glass coverslips whose orientation to the magnetic field could be varied through 180 degrees . The orientation dependence of the NMR spectrum was used to obtain an accurate measurement of the (13)C chemical shift..

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University of Melbourne Researchers