Rosiglitazone elicits in vitro relaxation in airways and precision cut lung slices from a mouse model of chronic allergic airways disease

Abstract

Rosiglitazone (RGZ), a peroxisome proliferator-activated receptor-γ (PPAR γ) ligand, is a novel dilator of small airways in mouse precision cut lung slices (PCLS). In this study, relaxation to RGZ and ẞ-adrenoceptor agonists were compared in trachea from naïve mice and guinea pigs and trachea and PCLS from a mouse model of chronic allergic airways disease (AAD). Airways were precontracted with methacholine before addition of PPAR γ ligands [RGZ, ciglitazone (CGZ), or 15-deoxy-Δ12,14-prostaglandin J2 (15-deoxy-PGJ2)] or ẞ-adrenoceptor agonists (isoprenaline and salbutamol). The effects of T0070907 and GW9662 (PPAR γ antagonists) or epithelial removal on relaxation were assessed. Changes in fo..