Journal article

The polarity protein Scrib mediates epidermal development and exerts a tumor suppressive function during skin carcinogenesis

Helen B Pearson, Edwina McGlinn, Toby J Phesse, Holger Schlueter, Anuratha Srikumar, Nathan J Goedde, Christina B Woelwer, Andrew Ryan, Wayne A Phillips, Matthias Ernst, Pritinder Kaur, Patrick Humbert



BACKGROUND: The establishment and maintenance of polarity is vital for embryonic development and loss of polarity is a frequent characteristic of epithelial cancers, however the underlying molecular mechanisms remain unclear. Here, we identify a novel role for the polarity protein Scrib as a mediator of epidermal permeability barrier acquisition, skeletal morphogenesis, and as a potent tumor suppressor in cutaneous carcinogenesis. METHODS: To explore the role of Scrib during epidermal development, we compared the permeability of toluidine blue dye in wild-type, Scrib heterozygous and Scrib KO embryonic epidermis at E16.5, E17.5 and E18.5. Mouse embryos were stained with alcian blue and aliza..

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Awarded by PCFA young investigator grant

Awarded by VCA/Richard Pratt Foundation Fellowship

Awarded by NHMRC

Awarded by CCV grant

Funding Acknowledgements

Many thanks to Joshua Noske, Olivia Cakebread, Samantha McIntosh, Jacinta Carter, Stephen Asquith, and the microscopy and histology core facility at the Peter MacCallum Cancer Centre for their technical assistance. This work was generously supported by the Prostate Cancer Foundation of Australia (PCFA), Richard Pratt Foundation, Victorian Cancer Agency (VCA), Ludwig Cancer Research, the Victorian State Government Operational Infrastructure Support, the Independent Research Institutes Infrastructure Support Scheme of the National Health and Medical Research Council Australia (NHMRC). The Australian Regenerative Medicine Institute is supported by grants from the State Government of Victoria and the Australian Government. HBP was supported by a PCFA young investigator grant (#YI1611) and a VCA/Richard Pratt Foundation Fellowship (RPF11_02). TJP was supported by a NHMRC project grant #1025239, ME was supported by a NHMRC Principal Research Fellowship #1079257. This work was also supported in part by CCV grant #807184 and NHMRC Senior Fellowship #509015 to PK and NHMRC project grant #1025874 to PK and HS. POH is supported by a NHMRC Career Development Fellowship.