Journal article

Visualising mouse neuroanatomy and function by metal distribution using laser ablation-inductively coupled plasma-mass spectrometry imaging

Bence Paul, Dominic J Hare, David P Bishop, Chad Paton, Tran Nguyen Van, Nerida Cole, Megan M Niedwiecki, Erica Andreozzi, Angela Vais, Jessica L Billings, Lisa Bray, Ashley I Bush, Gawain McColl, Blaine R Roberts, Paul A Adlard, David I Finkelstein, John Hellstrom, Janet M Hergt, Jon D Woodhead, Philip A Doble

CHEMICAL SCIENCE | ROYAL SOC CHEMISTRY | Published : 2015

Abstract

Metals have a number of important roles within the brain. We used laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) to map the three-dimensional concentrations and distributions of transition metals, in particular iron (Fe), copper (Cu) and zinc (Zn) within the murine brain. LA-ICP-MS is one of the leading analytical tools for measuring metals in tissue samples. Here, we present a complete data reduction protocol for measuring metals in biological samples, including the application of a pyramidal voxel registration technique to reproducibly align tissue sections. We used gold (Au) nanoparticle and ytterbium (Yb)-tagged tyrosine hydroxylase antibodies to assess the co-lo..

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Grants

Awarded by Australian Research Council's Linkage Projects program


Funding Acknowledgements

This project was supported by the Australian Research Council's Linkage Projects program (LP120200081), in conjunction with Agilent Technologies and ESI Ltd. We wish to thank Dr Christine Austin and Dr Nicole Jenkins for their helpful discussions. The Australian Research Council and the Victorian Government's Operational Infrastructure Support Program supported this research. We also wish to thank Jonas Marnell of Ethix Design for assistance with digital artwork.