Photoaffinity labelling of Plasmodium falciparum proteins involved in phospholipid transport

Abstract

Erythrocytes infected with mature-stage malaria parasites accumulate phospholipids from exogenous sources. We show that the transport of N-(7-nitrobenzy-2-oxa-1,3-diazol-4-yl)-1,2- ipalmitoyl-sn-glycero-3-phosphatidylethanolamine (N NBD-DPPE), from the erythrocyte membrane to the intracellular malaria parasite, is dependent upon metabolic energy, A photoreactive phospholipid analogue, N-[125I]iodo-4-azidosalicylamidy1-1,2-dilaury1-sn-glycero-3-p hosphatidylethanolamine (N-125I-ASA-DLPE), has been synthesised and used in an attempt to identify proteins involved in phospholipid trafficking in malaria-infected erythrocytes. This photoreactive probe was found to preferentially label a protein wi..