Journal article

The application of RAPD markers for potato cultivar identification

R Ford, PWJ Taylor

AUSTRALIAN JOURNAL OF AGRICULTURAL RESEARCH | C S I R O PUBLICATIONS | Published : 1997

Abstract

Where morphological techniques had failed, closely related potato cultivars were differentiated using random amplified polymophic DNA (RAPD) analysis based on the polymerase chain reaction. Total gemomic DNA was extracted from young sprout tissue from harvested tubers. Of 63 10-mer oligonucleotide primers screened, 51 primers produced a total of 256 amplification products of which 33 were polymorphic between the cultivars assessed. Polymorphic bands were selected to produce cultivar-specific markers to identify correctly suspect material in commercial plantings. Furthermore, a cultivar-specific RAPD marker was shown to be sufficiently robust and stringent for the identification of potential ..

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University of Melbourne Researchers