Journal article

LIPOPOLYSACCHARIDE PRIMING POTENTIATES CALCIUM IONOPHORE STIMULATED HUMAN PLACENTAL PROSTAGLANDIN-E2 RELEASE INVITRO

G WEI, GE RICE, M MICHAEL, SP BRENNECKE

EICOSANOIDS | SPRINGER | Published : 1992

Abstract

In this study, the effect of bacterial endotoxin (lipopolysaccharide; LPS) and of LPS priming on the in vitro release of PGE2 from human placental explants was investigated. Both LPS and the calcium ionophore A23187 significantly stimulated PGE2 release (P less than 0.05). Simultaneous exposure of placental explants to both LPS and A23187 revealed no additive or synergistic stimulation of PGE2 release. LPS priming of placental tissue significantly increased A23187-stimulated PGE2 release when compared to non-LPS-primed tissues. The addition of exogenous arachidonic acid (the substrate for PGE2 synthesis) also significantly (P less than 0.05) stimulated PGE2 release. There was, however, no si..

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