Journal article

Engineering temporal accumulation of a low recalcitrance polysaccharide leads to increased C6 sugar content in plant cell walls

Miguel E Vega-Sanchez, Dominique Loque, Jeemeng Lao, Michela Catena, Yves Verhertbruggen, Thomas Herter, Fan Yang, Jesper Harholt, Berit Ebert, Edward EK Baidoo, Jay D Keasling, Henrik V Scheller, Joshua L Heazlewood, Pamela C Ronald



Reduced cell wall recalcitrance and increased C6 monosaccharide content are desirable traits for future biofuel crops, as long as these biomass modifications do not significantly alter normal growth and development. Mixed-linkage glucan (MLG), a cell wall polysaccharide only present in grasses and related species among flowering plants, is comprised of glucose monomers linked by both β-1,3 and β-1,4 bonds. Previous data have shown that constitutive production of MLG in barley (Hordeum vulgare) severely compromises growth and development. Here, we used spatio-temporal strategies to engineer Arabidopsis thaliana plants to accumulate significant amounts of MLG in the cell wall by expressing the..

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Awarded by Office of Science, Office of Biological and Environmental Research of the U.S. Department of Energy

Awarded by Villum Fonden

Funding Acknowledgements

We thank Dr. Emilie Rennie for useful suggestions on sample purification for metabolite analysis. This work conducted by the Joint BioEnergy Institute was supported by the Office of Science, Office of Biological and Environmental Research, of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. J. H. was supported by the Villum Foundation's Young Investigator Program.