Analysis of plant nucleotide sugars by hydrophilic interaction liquid chromatography and tandem mass spectrometry
Jun Ito, Thomas Herter, Edward EK Baidoo, Jeemeng Lao, Miguel E Vega-Sanchez, A Michelle Smith-Moritz, Paul D Adams, Jay D Keasling, Bjoern Usadel, Christopher J Petzold, Joshua L Heazlewood
Analytical Biochemistry | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2014
Understanding the intricate metabolic processes involved in plant cell wall biosynthesis is limited by difficulties in performing sensitive quantification of many involved compounds. Hydrophilic interaction liquid chromatography is a useful technique for the analysis of hydrophilic metabolites from complex biological extracts and forms the basis of this method to quantify plant cell wall precursors. A zwitterionic silica-based stationary phase has been used to separate hydrophilic nucleotide sugars involved in cell wall biosynthesis from milligram amounts of leaf tissue. A tandem mass spectrometry operating in selected reaction monitoring mode was used to quantify nucleotide sugars. This met..View full abstract
Awarded by Office of Science, Office of Biological and Environmental Research, of the U.S. Department of Energy
Awarded by NSF-RCN
This work conducted by the Joint BioEnergy Institute was supported by the Office of Science, Office of Biological and Environmental Research, of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. The substrates UDP-xylose, UDP-arabinopyranose, and UDP-galacturonic acid were obtained from Carbosource Services (Athens, GA) which is supported in part by NSF-RCN Grant 0090281.