Isolation and Proteomic Characterization of the Arabidopsis Golgi Defines Functional and Novel Components Involved in Plant Cell Wall Biosynthesis
Harriet T Parsons, Katy Christiansen, Bernhard Knierim, Andrew Carroll, Jun Ito, Tanveer S Batth, Andreia M Smith-Moritz, Stephanie Morrison, Peter McInerney, Masood Z Hadi, Manfred Auer, Aindrila Mukhopadhyay, Christopher J Petzold, Henrik V Scheller, Dominique Loque, Joshua L Heazlewood
Plant Physiology | AMER SOC PLANT BIOLOGISTS | Published : 2012
The plant Golgi plays a pivotal role in the biosynthesis of cell wall matrix polysaccharides, protein glycosylation, and vesicle trafficking. Golgi-localized proteins have become prospective targets for reengineering cell wall biosynthetic pathways for the efficient production of biofuels from plant cell walls. However, proteomic characterization of the Golgi has so far been limited, owing to the technical challenges inherent in Golgi purification. In this study, a combination of density centrifugation and surface charge separation techniques have allowed the reproducible isolation of Golgi membranes from Arabidopsis (Arabidopsis thaliana) at sufficiently high purity levels for in-depth prot..View full abstract
Awarded by Office of Science, Office of Biological and Environmental Research, U.S. Department of Energy
This work was supported by the Office of Science, Office of Biological and Environmental Research, U.S. Department of Energy (contract no. DE-AC02-05CH11231) and by the Alexander von Hum-boldt Foundation (Feodor Lynen Research Fellowship to B.K.).