High Resolution Genotyping of Cryptosporidium by Mutation Scanning

Abstract

This chapter focuses on the high resolution genotyping of Cryptosporidium by mutation scanning. A range of different molecular approaches has been developed to characterize Cryptosporidium species and genotypes. Some of these approaches may not necessarily precisely resolve sequence variation because they depend on the size-separation of DNA molecules. Some methods are laborious and time-consuming to perform when the analysis of large numbers of samples is needed. Such limitations may be overcome by employing mutation detection methods, such as Single-Strand Conformation Polymorphism (SSCP). This chapter presents a study, the aim of that was to evaluate an SSCP approach for the genotyping of..