Journal article

Responses of activities, abundances and community structures of soil denitrifiers to short-term mercury stress

Zhifeng Zhou, Yuanming Zheng, Jupei Shen, Limei Zhang, Yurong Liu, Jizheng He

Journal of Environmental Sciences | SCIENCE PRESS | Published : 2012

Abstract

The responses of activities, abundances and community structures of soil denitrifiers to mercury (Hg) stress were investigated through a short-term incubation experiment. Four soil treatments with different concentrations of Hg (CK, Hg25, Hg50, and Hg 100, denoted as 0, 25, 50, and 100 mg Hg/kg dry soil, respectively) were incubated for 28 days. Soil denitrification enzyme activity (DEA) was measured at day 3, 7 and 28. The abundances and community structures of two denitrification concerning genes, nirS (cd(1)-nitrite reductase gene) and nosZ (nitrous oxide reductase gene), were analyzed using real-time PCR and denaturing gradient gel electrophoresis (DGGE). Results showed that soil DEA was..

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University of Melbourne Researchers

Grants

Awarded by Natural Science Foundation of China


Awarded by Ministry of Sciences and Technolog


Funding Acknowledgements

This work was supported by the Natural Science Foundation of China (No. 41020114001, 50921064), the Ministry of Sciences and Technology (No. 2009AA06Z402, 2008ZX07209-006-04) and the CAS/SAFEA International Partnership Program for Creative Research Teams of "Ecosystem Processes and Services".