Abundance and community structure of ammonia-oxidizing bacteria and archaea in a temperate forest ecosystem under ten-years elevated CO2
Xien Long, Chengrong Chen, Zhihong Xu, Ram Oren, Ji-Zheng He
Soil Biology and Biochemistry | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2012
Ammonia-oxidizing bacteria (AOB) and archaea (AOA) are considered as the key drivers of global nitrogen (N) biogeochemical cycling. Responses of the associated microorganisms to global changes remain unclear. This study was to determine if there was a shift in soil AOB and AOA abundances and community structures under free-air carbon dioxide (CO₂) enrichment (FACE) and N fertilization in Duke Forest of North Carolina, by using DNA-based molecular techniques, i.e., quantitative PCR, restriction fragment length polymorphism (RFLP) and clone library. The N fertilization alone increased the abundance of bacterial amoA gene, but this effect was not observed under elevated CO₂ condition. There was..View full abstract
This research was jointly supported by the Australian Research Council, the Chinese Academy of Sciences and the Natural Science Foundation of China. The Duke FACE research was supported by the Office of Science (BER), U.S. Department of Energy, through its FACE facilities program. The authors thank Jeffrey S Pippen and other staffs at DukeUniversity for their assistances in soil sampling.