Abundance and community composition of methanotrophs in a Chinese paddy soil under long-term fertilization practices
Yong Zheng, Li-Mei Zhang, Yuan-Ming Zheng, Hongjie Di, Ji-Zheng He
Journal of Soils and Sediments | SPRINGER HEIDELBERG | Published : 2008
As the second most important greenhouse gas, methane (CH ) is produced from many sources such as paddy fields. Methane-oxidizing bacteria (methanotrophs) consume CH in paddy soil and, therefore, reduce CH emission to the atmosphere. In order to estimate the contribution of paddy fields as a source of CH , it is important to monitor the effects of fertilizer applications on the shifts of soil methanotrophs, which are targets in strategies to combat global climate change. In this study, real-time polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) based on 16S rRNA and pmoA genes, respectively, were used to analyze the soil methanotrophic abundance and communit..View full abstract
Awarded by Chinese Academy of Sciences
Awarded by National Basic Research Program of China
Awarded by Natural Science Foundation of China
This work was supported by the Chinese Academy of Sciences (KZCX2- YW-408, KZCX1-YW-0603), the National Basic Research Program of China (2005CB121105), and the Natural Science Foundation of China (40701087, 50621804).