Journal article

Do land utilization patterns affect methanotrophic communities in a Chinese upland red soil?

Yong Zheng, Xinzhan Liu, Limei Zhang, Zhifeng Zhou, Jizheng He

Journal of Environmental Sciences | SCIENCE PRESS | Published : 2010

Abstract

Soil samples were collected from three plots under different land utilization patterns including degradation, farming, and restoration. The abundances of methanotrophs were quantified using real-time polymerase chain reaction (PCR) based on the pmoA and 16S rRNA genes, and the community fingerprint was analyzed using denaturing gradient gel electrophoresis (DGGE) aiming at pmoA gene. Significantly lower 16S rRNA and pmoA genes copies were found in the degradation treatment than in farming and restoration. Higher abundances of Type I than those of Type II methanotrophs were detected in all treatments. The treatment of farming was clearly separated from degradation and restoration according to..

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University of Melbourne Researchers

Grants

Awarded by Chinese Academy of Sciences


Awarded by Natural Science Foundation of China


Funding Acknowledgements

This work was supported by the Knowledge Innovation Program of Chinese Academy of Sciences (No KSCX2-YW-Z-1020, KZCX2-YW-JC401) and the Natural Science Foundation of China (No 40871129) We thank Zhiguo Li and Jiayang Song from Taoyuan Experimental Station of Agro-ecosystem Observation of Chinese Academy of Sciences for their assistance in soil sampling We would also like to thank the editor and two anonymous referees for their helpful comments on earlier versions of the manuscript