Journal article

Nitrogen loading levels affect abundance and composition of soil ammonia oxidizing prokaryotes in semiarid temperate grassland

Xin-Yi Shen, Li-Mei Zhang, Ju-Pei Shen, Ling-Hao Li, Chao-Lei Yuan, Ji-Zheng He

Journal of Soils and Sediments | SPRINGER HEIDELBERG | Published : 2011


Purpose: Global nitrogen deposition has profound impact on the terrestrial ecosystem including the semiarid temperate grassland, causing vegetation community shifts and soil acidification. Little is known regarding the effect of nitrogen (N) deposition on the belowground microbial communities. This study aimed to examine the response of ammonia-oxidizing bacteria (AOB) and archaea (AOA) to added N in semiarid temperate grassland. Materials and methods: We studied the changes of AOB and AOA by using molecular techniques targeting amoA genes along a urea fertilization gradient, i. e., 0, 1, 2, 4, 8, 16, 32, 64 g N m-2 year-1, in a 6-year field experiment of semiarid temperate grassland, Inner ..

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University of Melbourne Researchers


Awarded by Natural Science Foundation of China

Awarded by Chinese Academy of Sciences

Funding Acknowledgements

This work was supported by the Natural Science Foundation of China (41020114001, 41025004 and 40871129) and the Chinese Academy of Sciences (KZCX2-YW-JC401). We are grateful to the Duolun Restoration Ecological Experimentation and Demonstration Station for access to the study sites and research facilities.